Method for producing artemia nauplius from artemia sporangiocyst

Abstract

The method of incubating brine shrimp cyst to produce free swimming nauplius brine shrimp larva is to setting brine shrimp cyst and the additive in the weight ratio of 100 to 0.5-4 simultaneously into incubating culture medium. The additive is organic benzene containing one or several o-hydroxy radicals, such as pyrogallic acid, methyl pyrogallate, tannic acid, tanning extract, ea polyphenol, etc. The additive can damage the diapause mechanism of brine shrimp cyst to raise the brine shrimp cyst incubating within 24 hr to 90 %, and has flocculating effect to separate nauplius brine shrimp larva easily from un-incubated brine shrimp cyst. The present invention expands the application range of incubating culture medium and is especially suitable for incubating treatment of brine shrimp cyst the inland brine lake produces.

Description

(1) Technical field [0001] The invention relates to a production method for aquaculture live feed, in particular to a method for producing free-swimming Artemia nauplii from Artemia cysts. (2) Background technology [0002] Artemia nauplii are generally used as live feed in aquaculture, especially as live feed in the early stage of marine fish and shrimp larvae. There is generally no live feed such as Artemia nauplii on the market, only Artemia cysts, and the nauplii are hatched from Artemia cysts. Hatching of Artemia cysts can be done in hatching medium. Artemia cysts can survive for a long time or even several years after being fully dehydrated and in a dry environment with no oxygen and no light at low temperature. Long storage capacity and short time such as 24 hours can produce free-swimming nauplii characteristics, making it the most convenient source of live feed for aquaculture. [0003] Artemia cysts are collected from nature such as seaside salt pans and inland ...

AI Technical Summary

Problems solved by technology

The disadvantage of adopting hydrogen peroxide solution to process Artemia cysts is: 1) before use, for Artemia cysts of different Artemia strains or different batches of the same strain, a large number of experiments need to be carried out to determine the most suitable parameters, and in order to obtain Ideal hatching effect must be strictly controlled under this parameter during operation, which brings great inconvenience to users; 2) During the implementation process, it is necessary to transfer the hydrated Artemia cysts to hydrogen peroxide solution and corresponding 3) Artemia cysts must be washed with water in time to remove residual superfluous residues after passing through the hydrogen peroxide solution. Hydrogen oxidation, even need to be neutralized, which will inevitably increase the processing cost

Another treatment method using hydrogen peroxide is to directly add solid compounds capable of producing hydrogen peroxide, such as perborate, percarbonate, magnesium peroxide or calcium peroxide, to the incubation medium. The biggest disadvantage is that the pH value of the hatching medium changes greatly, and an excessively high pH value may cause the death of Artemia nauplii