Method for producing artemia nauplius from artemia sporangiocyst

A technology for Artemia nauplii and cysts, which is applied in the directions of biochemical equipment and methods, microorganisms, animal feed, etc., can solve the problem that the pH value of the incubation medium changes greatly, the operation process is cumbersome and complicated, and the Artemia nauplii die. and other problems, to achieve the effect of easy separation, low operation cost and improved hatching efficiency

Inactive Publication Date: 2007-12-05
TIANJIN OCEAN PAL CAROL BIOTECH
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of adopting hydrogen peroxide solution to process Artemia cysts is: 1) before use, for Artemia cysts of different Artemia strains or different batches of the same strain, a large number of experiments need to be carried out to determine the most suitable parameters, and in order to obtain Ideal hatching effect must be strictly controlled under this parameter during operation, which brings great inconvenience to users; 2) During the implementation process, it is necessary to transfer the hydrated Artemia cysts to hydrogen peroxide solution and corresponding 3) Artemia cysts must

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: The additive is pyrogallic acid (technically pure). Add 0.04 gram of pyrogallic acid in 4 grams of Artemia cysts, the weight ratio of Artemia cysts and additives is 100:1, the weight ratio of the total amount of Artemia cysts and additives to hatching medium is 0.404:100 , The hatching rate of Artemia cysts was measured to be 89%.

Embodiment 2

[0016] Embodiment 2: The additive is pine bark extract (proanthocyanidin content ≥ 95%, produced by Ningbo Traditional Chinese Medicine Pharmaceutical Factory). Add 0.05 gram of pine bark extract in 2 grams of Artemia cysts, the weight ratio of Artemia cysts and additives is 100: 2.5, the weight ratio of the total amount of Artemia cysts and additives to hatching medium is 0.205: 100 , The hatching rate of Artemia cysts was measured to be 92%.

Embodiment 3

[0017] Embodiment 3: The additive is a mixture of grape seed extract (proanthocyanidin content ≥ 95%, produced by Tianjin Jianfeng Natural Products Research and Development Co., Ltd.) and gallic acid (industrial pure). In 4 grams of Artemia cysts, add 0.08 grams of grape seed extract and 0.01 grams of gallic acid, the weight ratio of Artemia cysts and additives is 100: 2.25, the total amount of Artemia cysts and additives and the weight of the hatching medium The ratio was 0.409:100, and the hatching rate of Artemia cysts was measured to be 90%.

[0018] It can be seen from the test results of the above examples that Artemia cysts and additives are added to the hatching medium at the same time for 24 hours of hatching, and the hatching rate reaches about 90%, and the effect is remarkable;

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The method of incubating brine shrimp cyst to produce free swimming nauplius brine shrimp larva is to setting brine shrimp cyst and the additive in the weight ratio of 100 to 0.5-4 simultaneously into incubating culture medium. The additive is organic benzene containing one or several o-hydroxy radicals, such as pyrogallic acid, methyl pyrogallate, tannic acid, tanning extract, ea polyphenol, etc. The additive can damage the diapause mechanism of brine shrimp cyst to raise the brine shrimp cyst incubating within 24 hr to 90 %, and has flocculating effect to separate nauplius brine shrimp larva easily from un-incubated brine shrimp cyst. The present invention expands the application range of incubating culture medium and is especially suitable for incubating treatment of brine shrimp cyst the inland brine lake produces.

Description

(1) Technical field [0001] The invention relates to a production method for aquaculture live feed, in particular to a method for producing free-swimming Artemia nauplii from Artemia cysts. (2) Background technology [0002] Artemia nauplii are generally used as live feed in aquaculture, especially as live feed in the early stage of marine fish and shrimp larvae. There is generally no live feed such as Artemia nauplii on the market, only Artemia cysts, and the nauplii are hatched from Artemia cysts. Hatching of Artemia cysts can be done in hatching medium. Artemia cysts can survive for a long time or even several years after being fully dehydrated and in a dry environment with no oxygen and no light at low temperature. Long storage capacity and short time such as 24 hours can produce free-swimming nauplii characteristics, making it the most convenient source of live feed for aquaculture. [0003] Artemia cysts are collected from nature such as seaside salt pans and inland ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01K67/00C12N5/06A01K61/00
CPCA23K1/188A01K61/00A01K61/59A23K50/80Y02A40/81Y02A40/818
Inventor 樊昕宇马艳丽
Owner TIANJIN OCEAN PAL CAROL BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap