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Improved brine shrimp hatching and collecting method

A brine and incubator technology, which is applied in the field of improved brine shrimp hatching and collection, can solve the problems that juvenile fish cannot adapt to the water body environment, the density of feeding brine shrimp is low, and juvenile fish are easy to be infected and die, and the hatching conditions are easy to control and raw materials Environmentally friendly and easy to obtain, the effect of improving the hatching rate and survival rate

Inactive Publication Date: 2018-12-28
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During juvenile feeding, improper feeding can lead to mass mortality of juvenile fish
The main reasons are as follows: the density of feeding brine shrimp is low, and juvenile zebrafish die due to food shortage; feeding brine shrimp sometimes carries other pathogenic microorganisms, etc., and the juvenile fish are prone to infection and death; the survival rate of feeding brine shrimp Low, dead brine shrimp nutrient deterioration leads to the death of juvenile fish; in addition, dead brine shrimp leads to pollution of living water of juvenile fish, resulting in significant changes in pH and conductivity, and juvenile fish cannot adapt to the water environment and die

Method used

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  • Improved brine shrimp hatching and collecting method
  • Improved brine shrimp hatching and collecting method
  • Improved brine shrimp hatching and collecting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] An improved method for hatching and collecting brine shrimp, specifically comprising the following steps:

[0032] (1) Hatching preparation

[0033] Use a sodium hypochlorite solution with a concentration of 45ppm to aerate and sterilize the incubator (brand Beijing ESEN) for 3-4 hours, rinse the incubator repeatedly with clean water, and then add pure water to aerate and clean it for 2 hours. After cleaning, let it dry naturally.

[0034] (2) Hatch brine shrimp eggs

[0035] Screen the shrimp eggs used for hatching, and remove damaged and shriveled shrimp eggs. Accurately weigh 2g of shrimp eggs, about 3.49×10^5. Accurately weigh 10g of sea salt (the main components are NaCl, KCl, CaCl 2 , MgSO 4 ) into an ESEN incubator, add 1L of pure water to dissolve, adjust the pH to 9.5 with NaOH, adjust the temperature to 30°C with a constant temperature heater, and incubate for 22 hours.

[0036] (3) Collection, disinfection, tube loading and preservation of brine shrimp l...

Embodiment 2

[0043]Through the single factor experiment of four factors (temperature, pH, salinity, time) and five levels, the optimal level of each factor is obtained, and then the optimal level obtained by the single factor and the two levels before and after the optimal level are selected to carry out four factors and three levels Orthogonal experiment.

[0044] This embodiment 2 is compared with embodiment 1, except that the hatching conditions are hatched according to the orthogonal test scheme in the following table 1, the rest of the methods and steps are the same.

[0045] Table 1 Four-factor three-level orthogonal experimental design scheme

[0046]

[0047] The experimental results are shown in Table 2.

[0048] Table 2 Four-factor three-level orthogonal experiment results

[0049]

[0050] Result analysis

[0051] in Table 2 Respectively represent the sum of the hatching rate (the first one) and the survival rate (the second one) at each level of each factor. Due to ...

Embodiment 3

[0053] Compared with Example 1 in Example 3, the density of hatched shrimp eggs was hatched according to the following Table 3, and the rest of the hatching conditions were all using a temperature of 30°C, a pH value of 9.5, a salinity of 10‰, and a time of 22 hours. Other specific methods and steps are all the same as in Example 1.

[0054] Table 3 Effects of different shrimp egg densities on the hatching rate and survival rate of brine shrimp eggs

[0055]

[0056] It can be seen from Table 3 that when the density of shrimp eggs is 100-500 / mL, the hatching rate and survival rate are not significantly affected. When the density of shrimp eggs continues to increase to 1100 / mL, the hatching rate and survival rate tend to decline. It may be because the brine shrimp needs to be stripped when hatching. When the egg density is too high, the egg membrane will be deposited in the incubator, and the brine shrimp hatching time is 22 hours. The above reasons affect the hatching wate...

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Abstract

The invention discloses an improved brine shrimp hatching and collecting method. The method comprises the steps that a hatching device is disinfected and cleaned; hatching liquid is added to the hatching device, brine shrimp eggs are put into the hatching device, and hatching is conducted for 20 h-28 h at the temperature of 24 DEG C-32 DEG C, wherein the hatching liquid is a baysalt aqueous solution with the concentration of 0.5%-2.5% and the pH of 7.0-10; brine shrimp larvae are collected after hatching is completed; the collected brine shrimp larvae are disinfected, cleaned and collected toobtain brine shrimps. According to the method, a solution scheme is provided to overcome the defects that the hatching conditions of current brine shrimps have to be improved, standards of laboratorybrine shrimp hatching are not formed and hatched brine shrimp larva may have pathogenic microorganisms and obtain the brine shrimp larva with high hatching rate, high survival rate and high obtainingrate, the hatching operation of the brine shrimp is simple, the hatching conditions are easy to control, raw materials needed by hatching is environmentally-friendly and easy to obtain, and laboratorybrine shrimp hatching standards are formed.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to an improved method for hatching and collecting brine shrimp. Background technique [0002] Brine shrimp, also known as brine shrimp and fairy shrimp, are widely distributed in salt pans or salt lakes on land, and have high nutritional value. The winter eggs of brine shrimp are a very special kind of dormant eggs. The dormant eggs can be dried and canned, and sold in the form of commodities; they can also be hatched with brine shrimp eggs and used as high-quality live bait. Brine shrimp has many advantages. For example, it is easy to collect a large number of brine shrimp eggs and can be stored for several years; brine shrimp eggs hatch into nauplii, and it only takes about one day to cultivate them easily; as live feed, it is not easy to cause water quality deterioration; it does not sink, It has the ability to swim slowly; brine shrimp larvae are moderate in size and rich ...

Claims

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Application Information

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IPC IPC(8): A01K61/59A01K61/17A01K61/10A23K50/80A23K10/22
CPCA01K61/10A01K61/17A01K61/59A23K10/22A23K50/80Y02A40/81
Inventor 崔恒宓欧阳娟薛松磊沈晖孙振徐慧
Owner YANGZHOU UNIV
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