Application of zebra fish to testing water quality and toxicity and method for applying zebra fish to test water quality and toxicity
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A technology for zebrafish and zebrafish embryos, which is applied to biochemical equipment and methods, and the determination/inspection of microorganisms.
Active Publication Date: 2013-01-30
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI +1
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[0006] At present, there are too many indicators for testing water quality, and the project is relatively large, and it is impossible to measure many
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Embodiment 1
[0025] The screening of embodiment 1 optimal embryo culture fluid
[0026] 1.1 The preparation of different formulas of embryo culture medium and the effect of culturing zebrafish
[0027] A Prepare embryo culture solution containing the following concentrations of inorganic salts with ordinary water, adjust the pH to 7.2, culture zebrafish embryos for 72 hours, and observe the transparency and survival rate of zebrafish. The specific results are shown in Table 1:
[0028] Table 1 Effects of embryo culture medium prepared with ordinary water on zebrafish embryos
[0029]
[0030] B Use weakly alkaline water with a pH of 7.2 prepared by electrolysis of pure water to prepare an embryo culture solution containing the following concentrations of inorganic salts, culture zebrafish embryos for 72 hours, and observe the transparency and survival rate of zebrafish. The specific results are shown in Table 2:
[0031] Table 2 Effects of embryo culture medium prepared with weakly a...
Embodiment 2
[0034] Embodiment 2 zebrafish test water source toxicity
[0035] Add the water source to be tested with the embryo culture solution of the above-mentioned numbers A1 and B1 respectively, the volume ratio of the water source to be tested and the embryo culture solution is 1:3, and carry out the zebrafish toxicity test on the solution, wherein each treatment is repeated 5 times, The average value was taken, and the controls were embryo culture solutions A1 and B1 without the water source to be tested. The determination is mainly carried out through the following steps:
[0036] 1) The cultivation of zebrafish:
[0037] Sexually mature AB line common zebrafish were taken in separate tanks for male and female, and were raised in a circulating water culture system, with 14 hours of lighting and 10 hours of darkness alternately, the water temperature was kept at 28°C, and brine shrimp artificially incubated for 24 hours were regularly fed;
[0038] 2) Embryo selection:
[0039] ...
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Abstract
The invention relates to an application of zebra fish to testing water quality and toxicity and a method for applying zebra fish to test water quality and toxicity. The method is characterized by comprising the following steps of: preparing an embryo culture solution, then adding a water source to be tested to the embryo culture solution, using the embryo culture solution to which the water source to be tested is added to culture zebra fish embryos and then observing and recording the developmental aberration rates and mortality of the zebra fish embryos in 12-24 hours to judge the toxicity of the water source to be tested toward the embryos, wherein the ratio of the volume of the added water source to be tested to the volume of the embryo culture solution is 1:3; the embryo culture solution is directly prepared from alkalescent water with pH value being 6.7-8.5; and the embryo culture solution contains 5mM of NaCl, 0.17mM of KCl, 0.43mM of CaCl2 and 0.33mM of MgSO4. The method has the following beneficial effect that the embryo culture solution prepared from the alkalescent water ensures that the zebra fish embryos can quickly test the comprehensive toxicity of the water source to be tested, thus shortening the detection period.
Description
technical field [0001] The invention relates to the field of toxicity detection, in particular to a use and a method for testing water quality and toxicity of zebrafish. Background technique [0002] The comprehensive characteristics of water under the action of the environment, that is, the physical properties and chemical composition of water. Water in nature is an extremely complex complex composed of various substances (soluble and insoluble substances). The dissolved substances contained in water directly affect many properties of natural water, making the water quality different. [0003] To the deteriorating water source pollution problem, the way that the waterworks take is to add a large amount of chlorine (bleach powder) exceeding the standard to disinfect and sterilize. Although chlorine can kill various germs in water, once it combines with organic matter in water, it will produce a large amount of organic chloride (such as chloroform, dibromochloromethane) due...
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IPC IPC(8): C12Q1/02
Inventor 陈峰
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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