Method for screening 62 antibacterial drugs in livestock excrement

Abstract

The invention discloses a method for screening 62 antibacterial drugs in livestock excrement. The method specifically comprises the following steps: (1) preparing a standard working solution; (2) preprocessing a sample to be detected before analyzing; (3) drawing a substrate matching standard curve; (4) performing mass spectrometric analysis on the sample containing a certain amount of drugs in step (3) so as to obtain standard molecular ions and two characteristic ions of each drug; wherein the two characteristic ions include qualitative ions and quantitative ions; (5) performing qualitative analysis; (6) performing quantitative analysis. The screening method is precise, accurate, and high in sensitivity; the technical support is provided to detect the residues of antibiotics in a livestock farm and the surrounding environment and evaluate the potential risk and the influence of the antibiotics residual in the excrement to the environment. Therefore, the method is high in social benefit.

Description

technical field [0001] The invention belongs to the technical field of biotechnology detection, and in particular relates to a screening method for 62 kinds of antibacterial drugs in excrement of livestock and poultry. Background technique [0002] With the intensive development of animal husbandry, antibiotics are used more and more widely in the prevention and treatment of animal diseases. However, after antibiotics enter the animal body, except for a part that is absorbed, transformed and metabolized, and remains in animal tissues, most of them are in the form of prototypes. Or the form of metabolites is excreted with the feces, and the feces containing antibiotics are either used as organic fertilizers for planting, or directly discharged into the environment, resulting in the residual antibiotics in the feces entering the soil and water, which will affect the ecological system of the environment. It will have an important impact, and may affect the life activities of an...

AI Technical Summary

Problems solved by technology

As far as the environment is concerned, after the residual veterinary drugs are excreted into the external environment, they not only affect the flora in the soil through plant enrichment and affect the ecological balance, but also affect the activities of soil organisms, group structure and metabolic functions, and the number of ethnic elements. , so that the soil biota changes, the decomposition of microorganisms decreases, the soil structure changes, and the fertility decreases, and after the bacteria are inhibited by antibiotics, the fungi in the soil replace the bacteria, which makes the soil microbial population fundamentally change, resulting in difficult Containing biological "deserts"

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