Method for screening 62 antibacterial drugs in livestock excrement

A technology of livestock and poultry excrement and antibacterial drugs, which is applied in the field of biotechnology detection and can solve problems such as soil structure change, fertility decline, and microbial decomposition reduction.

Active Publication Date: 2017-12-19
河南省兽药饲料监察所
View PDF8 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as the environment is concerned, after the residual veterinary drugs are excreted into the external environment, they not only affect the flora in the soil through plant enrichment and affect the ecological balance, but also affect the activities of soil organisms, group structure and metabolic functions, and the number of ethnic elements. ,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for screening 62 antibacterial drugs in livestock excrement
  • Method for screening 62 antibacterial drugs in livestock excrement
  • Method for screening 62 antibacterial drugs in livestock excrement

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0136] A total of 11 categories of 62 kinds of antibacterial drugs were tested, including:

[0137] (a) Sulfonamides: sulfapyridine, sulfamethoxazole, sulfathiazole, sulfamethazine, sulfamethoxazole, sulfisoxazole, sulfamethiadiazole, benzosulfonamide, sulfamethoxazole, sulfamethoxazole Pyrimidine, sulfamethoxine, sulfamethoxypyridazine, sulfamethoxine, sulfachloropyridazine, sulfachloropyrazine, sulfaquinoxaline, sulfamethoxine, sulfamethoxine, sulfamethoxine pyrimidine, trimethoprim, ditrimethoprim;

[0138] (b) Quinolones: oxolinic acid, flumequine, cinoxacin, difloxacin, sarafloxacin, ofloxacin, nafloxacin, enrofloxacin, lomefloxacin, pefloxacin Star, norfloxacin, ciprofloxacin, nalidixic acid, enoxacin, gatifloxacin;

[0139] (c) Macrolides: tylosin, tilmicosin, roxithromycin, kitasamycin, azithromycin, clarithromycin, erythromycin;

[0140] (d) Quinoxalines: quinocetone, carbadoxy, acetylmethaquine;

[0141] (e) furans: furaltadone, furazolidone;

[0142] (f) Tetrac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Column lengthaaaaaaaaaa
Column inner diameteraaaaaaaaaa
Granularityaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for screening 62 antibacterial drugs in livestock excrement. The method specifically comprises the following steps: (1) preparing a standard working solution; (2) preprocessing a sample to be detected before analyzing; (3) drawing a substrate matching standard curve; (4) performing mass spectrometric analysis on the sample containing a certain amount of drugs in step (3) so as to obtain standard molecular ions and two characteristic ions of each drug; wherein the two characteristic ions include qualitative ions and quantitative ions; (5) performing qualitative analysis; (6) performing quantitative analysis. The screening method is precise, accurate, and high in sensitivity; the technical support is provided to detect the residues of antibiotics in a livestock farm and the surrounding environment and evaluate the potential risk and the influence of the antibiotics residual in the excrement to the environment. Therefore, the method is high in social benefit.

Description

technical field [0001] The invention belongs to the technical field of biotechnology detection, and in particular relates to a screening method for 62 kinds of antibacterial drugs in excrement of livestock and poultry. Background technique [0002] With the intensive development of animal husbandry, antibiotics are used more and more widely in the prevention and treatment of animal diseases. However, after antibiotics enter the animal body, except for a part that is absorbed, transformed and metabolized, and remains in animal tissues, most of them are in the form of prototypes. Or the form of metabolites is excreted with the feces, and the feces containing antibiotics are either used as organic fertilizers for planting, or directly discharged into the environment, resulting in the residual antibiotics in the feces entering the soil and water, which will affect the ecological system of the environment. It will have an important impact, and may affect the life activities of an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/884
Inventor 吴宁鹏吴志明彭丽孟蕾李慧素霍新远徐坤陈蔷
Owner 河南省兽药饲料监察所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap