Method for evaluating influence of pollutants on zebra fish biotoxicity

An evaluation method and biotoxicity technology, applied in biological testing, material testing products, measuring devices, etc., can solve the problems of low accuracy of zebrafish toxicity test results, time-consuming and laborious, and difficult operation of the full-cycle toxicity exposure test method. , to achieve the effect of reliable evaluation results, fast test process and easy operation

Pending Publication Date: 2020-05-22
CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

[0006] In view of this, the purpose of the present invention is to propose a method for evaluating the impact of pollutants on the biological toxicity of zebrafish, to solve the problem that the accuracy of the toxicity test results for zebrafish at a single growth stage is low, and there are operating problems in the full-cycle toxicity exposure test method. Difficult and time-consuming problems

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  • Method for evaluating influence of pollutants on zebra fish biotoxicity
  • Method for evaluating influence of pollutants on zebra fish biotoxicity
  • Method for evaluating influence of pollutants on zebra fish biotoxicity

Examples

Experimental program
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Embodiment 1

[0061] This embodiment is a method for evaluating the biological toxicity of difenoconazole (purchased from Beijing Bailingwei Technology Co., Ltd.) on zebrafish. The evaluation method includes:

[0062] Zebrafish embryo toxicity test:

[0063] Use reconstituted water to carry out the water-borne exposure test. First, conduct a pre-test to obtain the highest no-effect difenoconazole concentration of 0.1 mg / L and the lowest total lethal difenoconazole concentration of 4 mg / L;

[0064] Prepare gradient concentrations (0.5 mg / L, 1.0 mg / L, 1.5 mg / L, 2.0 mg / L, 2.5 mg / L) between the highest no-effect difenoconazole concentration and the lowest total lethal difenoconazole concentration , 3.0 mg / L) culture solution containing difenoconazole, and a reconstituted water blank control was set, then the zebrafish embryos were transferred to a 24-well plate, each plate used 20 wells, and each well contained 2ml culture solution and One zebrafish embryo, one plate is one repetition, and the...

experiment example

[0098] The evaluation method in Example 1 was used to evaluate and analyze the biological toxicity of the pollutants: pesticide azoxystrobin, hexavalent chromium, bisphenol F and dibutyl phthalate respectively, and determine the maximum effect of different pollutants on zebrafish respectively. Sensitive stage, and LC exposed for 96h in the most sensitive stage 50 Value and sub-lethal dose; Determine the results as shown in Table 2;

[0099] Table 2

[0100] group most sensitive stage LC 50 value

sublethal dose Azoxystrobin Larvae 0.35mg / L 0.10mg / L Bisphenol F Larvae 9.52mg / L 1.0mg / L Dibutyl phthalate embryo 2.86mg / L 0.05mg / L Hexavalent chromium adult fish 36.6mg / L 30mg / L

[0101] It can be seen from Table 2 that:

[0102] Different pollutants have different most sensitive stages to zebrafish. Therefore, it is difficult to scientifically evaluate its toxicity with only a single stage; and the most sensitive s...

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Abstract

The invention provides a method for evaluating the influence of pollutants on zebra fish biotoxicity. The method comprises the following steps of: respectively carrying out toxicity tests on zebra fish embryos, larva zebra fish and adult zebra fish to respectively obtain LC50 values and / or sub-lethal doses of zebra fish in different growth stages; selecting the zebra fish at a growth stage with the lowest LC50 value and / or sub-lethal dose as the most sensitive stage zebra fish; evaluating the toxicity strength of pollutants by adopting the LC50 value and / or the sub-lethal dose of the most sensitive stage zebra fish; according to the evaluation method, toxicity tests are carried out on zebra fish at different growth stages respectively, the most sensitive stage zebra fish can be determinedaccording to the biotoxicity difference of pollutants to zebra fish at different stages, and important guiding significance is achieved on zebra fish protection work by determining the most sensitivestage of zebra fish; in addition, the toxicity strength of pollutants is evaluated by the biotoxicity of zebra fish at the most sensitive stage, and the accuracy of pollutant toxicity evaluation is improved.

Description

technical field [0001] The invention relates to the technical field of pollutant toxicity evaluation, in particular to a method for evaluating the influence of pollutants on zebrafish biological toxicity. Background technique [0002] With the rapid development of China's industry and agriculture, more and more chemical pollutants can enter and coexist in the environment through various channels, and can enter the human body through food chain transmission and other channels. Most of these pollutants have toxic effects, which have caused serious impacts on the ecological environment and human health, especially the ecotoxic effects caused by toxic pollutants in water bodies need to be highly valued. [0003] As the most important vertebrate in the aquatic environment, fish are the main hazards of chemical pollutants. The abnormal growth, development and death of fish in the aquatic environment remind us that we must pay enough attention to the water environment pollution ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5014G01N33/5088G01N33/5044
Inventor 穆希岩李应仁刘佳袁立来黄瑛朱锋李绪兴
Owner CHINESE ACAD OF FISHERY SCI
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