The invention discloses a variable-period integrated signal damping ratio diagnosis method, which comprises the steps of firstly using a data acquisition instrument to acquire vibrating signals, inputting the vibrating signals into a computer for analysis, recognizing the intrinsic frequency of the signals through a non-prior base strategy, then establishing a constructor function through the intrinsic frequency, changing the time duration of signal analysis, performing double integration to the product of the signals and the constructor function, wherein the time duration of the double integration is in a twofold relation and is integer multiples of signal half period, and finally performing simultaneous two-section integration to obtain an equation to be solved, figuring out a signal attenuation coefficient, obtaining signal damping ratio through a relationship calculation formula of the attenuation coefficient, the intrinsic frequency and the damping ratio, and outputting a diagnosis result. The variable-period integrated signal damping ratio diagnosis method has the advantages that the diagnosis speed is fast, the influence of negative frequency items on damping diagnosis is eliminated through variable-period integration during calculation, the accuracy of the diagnosis result is high, the diagnosis process is not restricted by the magnitude of the damping value and the engineering application value is very great.