Monitoring and identifying evaluation method for group biotoxicity in printing and dyeing wastewater

A technology for printing and dyeing wastewater and biotoxicity, which is used in chemiluminescence/bioluminescence, color/spectral property measurement, and testing water, etc. Effect

Inactive Publication Date: 2018-06-05
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem that the results of a single biological toxicity test are not representative, the present invention proposes that a group of biological toxicity tests can objectively reflect the safety of wastewater. In order to directly characterize the toxicity of wastewater, it is necessary to develop a comprehensive and comprehensive toxicity evaluation index

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  • Monitoring and identifying evaluation method for group biotoxicity in printing and dyeing wastewater
  • Monitoring and identifying evaluation method for group biotoxicity in printing and dyeing wastewater
  • Monitoring and identifying evaluation method for group biotoxicity in printing and dyeing wastewater

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Experimental program
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Embodiment 1

[0044] (1) Photobacteria, zebrafish larvae, zebrafish embryos and chlorella were used as the test organisms.

[0045] (2) Make a group biological toxicity test with four kinds of organisms as subjects.

[0046](3) Preparation of bacterial suspension: freeze-dried powder of Photobacterium brighten stored at 4°C was revived in 1mL 2.5% NaCl solution stored at 4°C for 3 minutes, and the bacteria immediately glowed, and then added 9mL of 3% NaCl stored at 4°C When the NaCl solution arrives, shake it at a constant temperature of 20°C for 20 minutes after mixing, make a bacterial suspension with the required concentration with 3% NaCl, and measure the initial luminescence intensity with a microplate multifunctional detector.

[0047] (4) The maximum absorption peak and the initial inoculation concentration of Chlorella: the maximum absorption wavelength of the algae liquid is 685nm, and the initial inoculation absorbance of the algae liquid is 0.06.

[0048] (5) Exposure to test or...

Embodiment 2

[0065] (1) Photobacteria, zebrafish larvae, zebrafish embryos and chlorella were used as test organisms.

[0066] (2) Make a group biological toxicity test with four kinds of organisms as subjects.

[0067] (3) Preparation of bacterial suspension: freeze-dried powder of Photobacteria brightens stored at 4°C was revived in 1mL 2.5% NaCl solution stored at 4°C for 3 minutes, the bacteria immediately glowed, and added to 9mL of 4.5% NaCl stored at 4°C When the NaCl solution arrives, shake it at a constant temperature of 20°C for 20 minutes after mixing, make a bacterial suspension with the required concentration with 3% NaCl, and measure the initial luminescence intensity with a microplate multifunctional detector.

[0068] (4) The maximum absorption peak and the initial inoculation concentration of Chlorella: the maximum absorption wavelength of the algae liquid is 685nm, and the initial inoculation absorbance of the algae liquid is 0.065.

[0069] (5) Exposure to test organism...

Embodiment 3

[0086] (1) Photobacteria, zebrafish larvae, zebrafish embryos and chlorella were used as test organisms.

[0087] (2) Make a group biological toxicity test with four kinds of organisms as subjects.

[0088] (3) Preparation of bacterial suspension: freeze-dried powder of Photobacterium luminosus preserved at 4°C was recovered in 1mL of 2.5% NaCl solution preserved at 4°C for 3 minutes, and the bacteria immediately glowed, and then added to 9mL of 5% NaCl preserved at 4°C When the NaCl solution arrives, shake it at a constant temperature of 20°C for 20 minutes after mixing, make a bacterial suspension with the required concentration with 3% NaCl, and measure the initial luminescence intensity with a microplate multifunctional detector.

[0089] (4) The maximum absorption peak and the initial inoculation concentration of Chlorella: the maximum absorption wavelength of the algae liquid is 685nm, and the initial inoculation absorbance of the algae liquid is 0.07.

[0090] (5) Expo...

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Abstract

The invention discloses a monitoring and identifying evaluation method for group biotoxicity in printing and dyeing wastewater and belongs to the field of biotoxicity identification. The method comprises the following steps: screening photogenic bacteria, zebrafish larvae, zebrafish embryos and chlorella as test organisms; and performing risk and toxicity reduction evaluation on the printing and dyeing wastewater by adopting group biotoxicity tests and combining with a toxicity evaluation method, and constructing a toxicity identification evaluation system by combining with a TIE technology according to the wastewater contamination characteristics and results. According to the method disclosed by the invention, the group biotoxicity tests comprising three trophic levels such as decomposer,producer and consumer are performed for characterizing toxicities of the printing and dyeing wastewater, and the problem that a single biotoxicity test does not have representativeness is solved. Themethod has the advantages of being comprehensive, simple, high-efficiency, capable of controlling from the source, high in safety and the like, can be widely applied to researching and evaluating influences of toxic pollutants on the ecological environment, and provides a certain basis for ecological risk assessment.

Description

technical field [0001] The invention relates to the field of biological toxicity identification, in particular to a group biological toxicity monitoring and identification evaluation method in printing and dyeing wastewater. Background technique [0002] In recent years, with the rapid development of China's textile industry, the water ecological safety of printing and dyeing wastewater has attracted more and more people's attention. Many researchers also realized the lack of physical and chemical monitoring, and gradually combined biological toxicity test with physical and chemical analysis to evaluate the ecological risk of wastewater. However, the results of a single biotoxicity test are not representative, and a group of biotoxicity tests can objectively reflect the safety of wastewater. In addition, in order to directly characterize the toxicity of wastewater, it is necessary to develop a comprehensive and comprehensive toxicity evaluation index. Finally, due to the c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18G01N21/76G01N21/33
CPCG01N21/33G01N21/763G01N33/186G01N33/1866
Inventor 王艺璇赵远陈文艳孙辰鹏符菁胡茜张艺张芸冀云
Owner CHANGZHOU UNIV
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