Method for rapidly evaluating damage effect on hepatic function of zebra fishes by compounds
A liver function and compound technology, applied in the field of toxicology detection, can solve the problems of large error in experimental results, large error in liver area selection, long experimental cycle, etc., achieve stable and reliable repeatability, improve experimental efficiency, and reduce experimental cost. Effect
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Embodiment 1
[0064] Example 1: Evaluation of the toxic effect of carbaryl on zebrafish liver
[0065] 1. Acquisition and Use of Juvenile Zebrafish
[0066] Use healthy and sexually mature zebrafish whose liver specifically expresses fluorescence, put them in a mating tank at a ratio of 1 / 1 or 1 / 2, place a partition in the middle, and place it in a dark environment. Remove the partition before turning on the light the next day , stimulated by light to ovulate, fish out the adult fish half an hour later, and control the ovulation time within half an hour to reduce the difference in development time between embryos. Fertilized eggs were collected, sterilized and cleaned, then transferred into zebrafish embryo culture water, and 0.2ppm methylene blue was added to the culture water, and light-controlled culture was performed at 28°C. Add 0.2mM phenylthiourea 12 hours after the birth of the zebrafish embryos, change about 1 / 2 of the water every 24 hours in the middle, and suck out the dead embr...
Embodiment 2
[0094] Example 2: Evaluation of the toxic effect of isoniazid on zebrafish liver
[0095] 1. Acquisition and Use of Juvenile Zebrafish
[0096] Use healthy and sexually mature zebrafish whose liver specifically expresses fluorescence, put them in a mating tank at a ratio of 1 / 1 or 1 / 2, place a partition in the middle, and place it in a dark environment. Remove the partition before turning on the light the next day , stimulated by light to ovulate, fish out the adult fish half an hour later, and control the ovulation time within half an hour to reduce the difference in development time between embryos. Fertilized eggs were collected, sterilized and cleaned, then transferred into zebrafish embryo culture water, and 0.2ppm methylene blue was added to the culture water, and light-controlled culture was performed at 28°C. Add 0.2 mM phenylthiourea 12 hours after the birth of zebrafish embryos, change about 1 / 2 of the water every 24 hours, and suck out dead embryos in time. The 4-...
Embodiment 3
[0120] Example 3: Evaluation of the toxic effect of pyrazinamide on zebrafish liver
[0121] 1. Acquisition and Use of Juvenile Zebrafish
[0122] Use healthy and sexually mature zebrafish embryos whose liver specifically expresses fluorescence, put them in a mating tank at a ratio of 1 / 1 or 1 / 2, place a partition in the middle, and place it in a dark environment. Remove the partition before turning on the light the next day. Plates, stimulated by light to ovulate, fish out the adult fish half an hour later, and control the ovulation time within half an hour to reduce the difference in development time between embryos. Fertilized eggs were collected, sterilized and cleaned, then transferred into zebrafish embryo culture water, and 0.2ppm methylene blue was added to the culture water, and light-controlled culture was performed at 28°C. Add 0.2 mM phenylthiourea 12 hours after the birth of zebrafish embryos, change about 1 / 2 of the water every 24 hours, and suck out dead embryo...
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