5 results about "Laser Scanning Microscopy" patented technology

The present application discloses a multiphoton microscopic imaging probe, an imaging device, and an imaging method. The imaging probe comprises: a medium assembly, wherein the medium assembly comprises a solid elastic medium and an imaging window plate that carries the solid elastic medium and is used for being in contact with an imaging object; a handle-shaped housing, wherein the medium assembly is detachably arranged at a first end of the handle-shaped housing; and an imaging module based on miniature laser scanning microscopy technology, wherein the imaging module is arranged in the handle-shaped housing, connected to a photoelectric composite cable and used for transmitting and controlling exciting light and receiving a fluorescence signal; and the imaging module comprises an objective lens in contact-fit with the solid elastic medium. Therefore, the objective lens provided by the present application comes into contact with the imaging object on the basis of the solid medium, eliminating the limitations of conventional immersion media on microscopic imaging apparatuses, thereby allowing the imaging probe to come into contact with the imaging object at any angle.

The present invention relates to a method for multidimensional evaluation of the similarity of a sample to breast milk, wherein the method is performed in the following steps: (1) establishing a local breast milk phospholipid database; (2) collecting and pretreating a sample to be evaluated; (3) determining the contents of characteristic phospholipid subclasses, the contents of characteristic phospholipid molecules in the sample to be evaluated, and obtaining a microstructural image of milk fat globules in the sample by confocal laser scanning microscopy; (4) calculating the score G for the sample to be evaluated. The present invention evaluates the similarity of samples to breast milk based on phospholipids in three aspects: the composition of characteristic phospholipid subclasses, the composition of characteristic phospholipid molecules, and the morphological similarity evaluation, so that the scheme can better evaluate the simulation quality of infant formulas and their raw and auxiliary materials to breast milk based on phospholipids.

The application discloses a reflection type beam combining field splicing system, a multi-channel laser scanning beam combining system and a laser scanning microscope, and relates to the technical field of microscopic imaging instruments.The reflection type beam combining field splicing system comprises at least one reflection type beam combining subunit, and the reflection type beam combining subunit comprises a relay lens group and an adjustable mirror.The relay lens group comprises two 4f systems, the pupil conjugate planes of the starting points of each 4f system are different, and there is a focal point conjugate plane in the internal optical space between the two 4f systems.The adjustable mirror is arranged in the internal optical space, receives a light beam from a first light path and reflects the light beam, and simultaneously allows a light beam from a second light path to pass unobstructed.The first light path light beam reflected by the adjustable mirror and the straight-through second light path light beam complete beam combining field splicing at the focal point conjugate plane and converge at the same pupil conjugate plane.The reflection type beam combining field splicing system can simplify the light path structure and reduce the transmission loss of laser energy.

A method and a system for removing particles during laser-based scanning microscopy are disclosed. The method comprises: scanning a sample (12) in a medium (19) with focused laser light along a plurality of scanning areas (21) and thereby determining a plurality of measurement values, each indicating scattered light from a corresponding one of the scanning areas (21); determining an occurrence of a particle (30) in an active scanning area (22) based on comparing values of a measurement values subset which corresponds to a scanning areas subset (32) of spatially connected scanning areas containing the active scanning area (22); in reaction to having determined the occurrence of the particle (30) in the active scanning area (22), removing the particle (30) from the active scanning area (22), comprising one of: providing a relative movement of the focused laser light from the active scanning area (22) to outside the scanning areas subset (32) and subsequently lowering a laser power of the focused laser light below an optical trapping threshold, and lowering the laser power of the focused laser light below the optical trapping threshold and providing a flow of the medium (19) in the active scanning area (22).

A laser scanning microscopy system includes a laser whose beam is modified by an acousto-optic deflector that includes an optical element having a surface with one or more steps formed thereon; a conductive layer formed on the surface with the steps; one or more crystals secured to each step; and electrodes positioned on each surface of each crystal.