3 results about "Laser Scanning Microscopy" patented technology

The present application discloses a multiphoton microscopic imaging probe, an imaging device, and an imaging method. The imaging probe comprises: a medium assembly, wherein the medium assembly comprises a solid elastic medium and an imaging window plate that carries the solid elastic medium and is used for being in contact with an imaging object; a handle-shaped housing, wherein the medium assembly is detachably arranged at a first end of the handle-shaped housing; and an imaging module based on miniature laser scanning microscopy technology, wherein the imaging module is arranged in the handle-shaped housing, connected to a photoelectric composite cable and used for transmitting and controlling exciting light and receiving a fluorescence signal; and the imaging module comprises an objective lens in contact-fit with the solid elastic medium. Therefore, the objective lens provided by the present application comes into contact with the imaging object on the basis of the solid medium, eliminating the limitations of conventional immersion media on microscopic imaging apparatuses, thereby allowing the imaging probe to come into contact with the imaging object at any angle.

A method and a system for removing particles during laser-based scanning microscopy are disclosed. The method comprises: scanning a sample (12) in a medium (19) with focused laser light along a plurality of scanning areas (21) and thereby determining a plurality of measurement values, each indicating scattered light from a corresponding one of the scanning areas (21); determining an occurrence of a particle (30) in an active scanning area (22) based on comparing values of a measurement values subset which corresponds to a scanning areas subset (32) of spatially connected scanning areas containing the active scanning area (22); in reaction to having determined the occurrence of the particle (30) in the active scanning area (22), removing the particle (30) from the active scanning area (22), comprising one of: providing a relative movement of the focused laser light from the active scanning area (22) to outside the scanning areas subset (32) and subsequently lowering a laser power of the focused laser light below an optical trapping threshold, and lowering the laser power of the focused laser light below the optical trapping threshold and providing a flow of the medium (19) in the active scanning area (22).

A laser scanning microscopy system includes a laser whose beam is modified by an acousto-optic deflector that includes an optical element having a surface with one or more steps formed thereon; a conductive layer formed on the surface with the steps; one or more crystals secured to each step; and electrodes positioned on each surface of each crystal.