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Novel fluorescent molecular probe and application thereof in field of Al (III) detection

A fluorescent molecular probe, a new type of technology, used in fluorescence/phosphorescence, material excitation analysis, organic chemistry, etc., can solve the problems of complex synthesis steps, unsuitable for industrialization, and lack of price competitiveness, and achieve high selectivity and The effect of sensitivity

Inactive Publication Date: 2012-11-07
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In recent years, the fluorescent molecular probe method can be effectively applied to the determination of metal content in the environment and living systems, such as the fluorescent molecular probe formed by the combination of anthraquinone and macrocycle with the following structure, which can be used for the detection of Al(III) in solution Spectroscopic and electrochemical dual-mode detection [Org.Lett., 2010, 12(3): 560-563], but it has not been used for laser confocal cell fluorescence imaging, and the fluorescent molecular detection formed by the combination of anthraquinone and macrocycle Due to the complex synthesis steps and high cost, it is not suitable for industrial promotion and has no price competitiveness

Method used

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  • Novel fluorescent molecular probe and application thereof in field of Al (III) detection
  • Novel fluorescent molecular probe and application thereof in field of Al (III) detection
  • Novel fluorescent molecular probe and application thereof in field of Al (III) detection

Examples

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Effect test

Embodiment 1

[0026] In this embodiment, the fluorescent molecular probe has the following molecular structural formula:

[0027]

[0028] The fluorescent molecular probe was dispersed in dimethyl sulfoxide-water (volume ratio 1:9) at a concentration of 1 μM and 5 μM Al(III), incubated with HeLa cells, and observed with an Olympus IX71 fluorescence microscope (excitation at 400 nm) and collect as figure 1 Fluorescent images shown.

Embodiment 2

[0030] In this embodiment, the fluorescent molecular probe has the following molecular structural formula:

[0031]

[0032] The fluorescent molecular probe was dispersed in dimethyl sulfoxide-water (volume ratio 1:9) at a concentration of 1 μM and 5 μM Al(III), incubated with HeLa cells, and observed with an Olympus IX71 fluorescence microscope (excitation at 400 nm) and collect as figure 2 Fluorescent images shown.

Embodiment 3

[0034] In this embodiment, the fluorescent molecular probe has the following molecular structural formula:

[0035]

[0036] The fluorescent molecular probe was dispersed in DMSO-water (volume ratio 1:9) at a concentration of 1 μM and 5 μM Al(III) to selectively identify and measure Al(III). The identification of is achieved by observing its fluorescent color change.

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Abstract

The invention discloses a novel fluorescent molecular probe, which has a general formula with the following structure, wherein R1 is an alkyl chain having 1-8 carbon atoms, and R2 is hydrogen, a sulfonic acid group, a sulfonic acid ion group or a 3-15 carbon atom-containing quaternary ammonium group which is connected to a position 2,3,5 or 6 of an intermediate benzene ring. The invention also discloses application of the novel fluorescent molecular probe in the field of Al (III) detection. The novel fluorescent molecular probe provided by the invention has higher selectivity and sensitivity on Al (III). Through combining confocal laser scanning microscopy, fluorescence imaging of the fluorescent molecular probe after Al (III) is captured in cells can also be obtained.

Description

technical field [0001] The invention relates to the technical field of fluorescent molecular probes, in particular to a novel fluorescent molecular probe and its application in the field of Al(III) detection. Background technique [0002] In the research of life science, metal ions occupy an important position. Real-time online detection of the concentration and distribution of metal ions in the living system, and the exploration of the role played by various metal ions will provide the necessary basis for biological and medical research. Aluminum is the most abundant metal in nature, and it is of great significance to develop a method to realize the visible spectrum analysis of aluminum ions in the living system. [0003] People have designed and synthesized fluorescent molecules that can effectively express molecular recognition information through the fluorescent signal of the molecule. In a certain system, when a certain physical or chemical property in the substance or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D213/36G01N21/64
Inventor 孟庆华刘恒程森任吉存向德成
Owner SHANGHAI JIAO TONG UNIV
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