A whitening and anti-aging composition containing bakuchiol and vitamin c and a preparation method thereof

Abstract

The application belongs to the technical field of cosmetics, and particularly relates to a whitening and anti-aging composition containing psoralen and vitamin C and a preparation method thereof. The whitening and anti-aging composition provided by the application is composed of scutellaria baicalensis georgi extract, white pool flower seed oil, psoralen and vitamin C, has a rapid and efficient whitening and anti-aging effect, is mild and non-irritating, and can be used for preparing skin care products with whitening and anti-aging effects. In the preparation of the scutellaria baicalensis georgi extract, a deep eutectic solvent combined with a pulsed direct current field treatment is used, which has the advantages of low cost, high extraction efficiency, simple operation, non-toxicity, good biocompatibility, biodegradability and the like. The preparation method of the whitening and anti-aging composition provided by the application is simple and easy to operate, is beneficial to industrialized production and application, and has good market application value.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a whitening and anti-aging composition containing bakuchiol and vitamin C and a preparation method thereof. BACKGROUND

[0002] Skin is the largest organ of the human body, and with the growth of age, the metabolic activity of cells decreases. Aging is a natural aging rule in the process of life, and particularly the exposed skin contains a large amount of sebum, is constantly in contact with oxygen, and is often attacked by ultraviolet radiation and the like. Free radicals can react with biological molecules of the skin, causing lipid peroxidation damage, resulting in damage and aging of the skin, which will gradually become rough, wrinkled, darkened, and some age spots will grow. In addition, excessive sugar intake, irregular diet, long-term sleep deprivation, and endocrine disorders can also cause skin aging phenomena such as yellow-brown spots, wrinkles, collagen loss, and sagging skin on the face.

[0003] At present, there are various whitening and anti-aging cosmetics on the market, such as reducing melanin formation by inhibiting tyrosinase activity, improving pigmentation, chloasma and the like; improving antioxidant properties by removing excess free radicals in cells; improving skin elasticity and tightening the skin by promoting collagen production; and improving antioxidant, anti-inflammatory or whitening effects by adding plant extracts. However, the effects of existing whitening and anti-aging cosmetics are uneven, and there is generally no obvious short-term effect in inhibiting tyrosinase activity, removing free radicals, and promoting collagen production, and the long-term use effect is also not ideal. Strong whitening ingredients can cause damage to the skin barrier, and after discontinuation, the skin is more susceptible to external stimuli damage, causing pigmentation, or causing water loss, and dry skin is more prone to pigment deposition. For products mainly containing plant extracts, the same raw materials, different preparation methods, product efficacy direction and action strength are different, and the compounding ratio also affects the product efficacy.

[0004] Furthermore, most of the products on the current market tend to focus only on single whitening or anti-aging effect, and cannot achieve comprehensive skin care. At the same time, those products that can simultaneously consider whitening and anti-aging are few and most of them are irritating, and cannot meet the needs of people for whitening and anti-aging products. Therefore, in order to better realize product upgrading, it is the common goal in the field of whitening and anti-aging cosmetics to develop a whitening and anti-aging composition with rapid and efficient whitening, anti-inflammatory and antioxidant effects and mildness and no irritation. SUMMARY

[0005] In view of the above defects or improvement needs of the prior art, the present application provides a whitening and anti-aging composition containing bakuchiol and vitamin C and a preparation method thereof, which solves the problems of single, poor and strong irritation of existing whitening and anti-aging products.

[0006] To achieve the above object, the present application adopts the following technical solutions:

[0007] In a first aspect, the present application provides a whitening and anti-aging composition containing bakuchiol and vitamin C, which comprises the following components by weight: 12-17 parts of scutellaria extract, 9-13 parts of white pool flower seed oil, 5-9 parts of bakuchiol, and 3-7 parts of vitamin C.

[0008] The preparation method of the scutellaria extract specifically comprises the following steps:

[0009] A1, dry scutellaria is pulverized and sieved to obtain scutellaria powder;

[0010] A2, the hydrogen bond acceptor and the hydrogen bond donor are mixed and heated and stirred to obtain a viscous liquid, and then ultrapure water is added and stirred uniformly to obtain a deep eutectic solvent with a water content of 30-40%;

[0011] A3, the scutellaria powder is added to the deep eutectic solvent and subjected to pulsed direct current field treatment, and then centrifuged, filtered, and the supernatant is concentrated to obtain the scutellaria extract;

[0012] The hydrogen bond acceptor is matrine, the hydrogen bond donor is glycerol glucoside, and the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:1.5-3.

[0013] The scutellaria extract contains flavonoids, volatile oils, polysaccharides, terpenes, phenolic acids, phenylethanol, amino acids, sterols, and trace elements, and has the effects of anti-inflammatory, antioxidant, antibacterial, antiviral, antitumor, and immunomodulation.

[0014] The white pool flower seed oil is extracted from the seeds of white pool flowers and has more than 94% long-chain fatty acids, so it has excellent lubricating and regulating properties; at the same time, it also has excellent antioxidant properties and stability, and can be used as an effective carrier for other active ingredients.

[0015] Bakuchiol is an isoprene phenolic terpenoid compound extracted mainly from psoralea corylifolia, which has the effects of whitening, anti-aging, anti-acne, anti-inflammatory, antioxidant, and antibacterial, and is called "plant retinol" due to its high anti-aging ability and low irritation.

[0016] Vitamin C, also known as L-ascorbic acid, is a commonly used whitening raw material, which can interact with copper ions in the active site of tyrosinase to slow down the synthesis of dopaquinone and other melanins, thereby interfering with the formation of melanin and achieving the effect of whitening and freckle removal.

[0017] Deep eutectic solvents are eutectic mixtures formed by natural compounds through non-covalent interactions, with much lower melting points than individual compounds; have the advantages of low cost, easy synthesis, non-toxicity, good biocompatibility, biodegradability, etc., and also have highly adjustable physicochemical properties, which can be optimized by changing the type or ratio of hydrogen bond donors and acceptors to optimize the basic properties such as viscosity, solubility, polarity, etc., to meet the needs of different treatments.

[0018] The pulsed direct current field has high energy, which can effectively break the cell wall and tissue structure of plants, promote the full contact of deep eutectic solvents and active ingredients, and help to accelerate the release of target substances, thereby improving the dissolution rate and extraction efficiency of active ingredients.

[0019] Preferably, the whitening and anti-aging composition comprises, by weight parts, the following components: 15 parts of scutellaria extract, 11 parts of white pool flower seed oil, 7 parts of psoralen, and 5 parts of vitamin C.

[0020] Preferably, the molar ratio of the hydrogen bond acceptor and the hydrogen bond donor is 1:2.2.

[0021] Preferably, the temperature of heating and stirring in step A2 is 80-100℃, the stirring speed is 400-550rpm, and the heating and stirring time is 2-3h.

[0022] Preferably, the solid-liquid ratio of scutellaria powder and deep eutectic solvent in step A3 is 1g:25-40mL.

[0023] Preferably, the temperature of pulsed direct current field treatment in step A3 is 25-35℃, and the time is 40-60min.

[0024] Preferably, the specific conditions of the pulsed direct current field in step A3 are: peak voltage 27-32V, pulse frequency 1.2-1.8kHz, and duty cycle 0.3-0.7.

[0025] In a second aspect, the present application provides a preparation method of the whitening and anti-aging composition of the first aspect, which comprises the following steps: weighing each component according to the formula amount, mixing and homogenizing to obtain the whitening and anti-aging composition.

[0026] In a third aspect, the present application provides the use of the whitening and anti-aging composition of the first aspect or the whitening and anti-aging composition prepared by the preparation method of the second aspect in the preparation of skin care products with whitening and anti-aging effects.

[0027] Preferably, the skin care product is serum, jelly, emulsion, mask or cream.

[0028] The beneficial effects of the present application are:

[0029] (1) The present application provides a whitening and anti-aging composition containing bakuchiol and vitamin C, which is composed of scutellaria extract, white pool flower seed oil, bakuchiol and vitamin C, and has rapid and efficient whitening and anti-aging effects.

[0030] (2) The present application provides a preparation method of scutellaria extract, which adopts a deep eutectic solvent combined with pulse direct current field treatment, and has the advantages of low cost, high extraction efficiency, simple operation, non-toxicity, good biocompatibility and biodegradability.

[0031] (3) The present application provides a preparation method of the whitening and anti-aging composition, which is simple and easy to operate, and is conducive to industrialized production and application.

[0032] (4) The present application provides the application of the whitening and anti-aging composition in preparing products with whitening and anti-aging effects, which has good market application value. DETAILED DESCRIPTION

[0033] In order to further illustrate the technical means and effects adopted by the present application to achieve the predetermined invention purposes, the specific embodiments, structures, features and effects of the present application according to the composition are described in detail as follows.

[0034] The experimental methods not specified in the following compositions are usually carried out under conventional conditions or according to the conditions recommended by the manufacturers. The materials, reagents, etc. used, if not specifically stated, can be obtained from commercial channels.

[0035] Some raw materials and sources are as follows:

[0036] The scutellaria extract is purchased from Gansu Yui Sen Pharmaceutical Technology Co., Ltd.;

[0037] The white pool flower seed oil is purchased from Sumi Chemicals (Guangzhou) Co., Ltd.;

[0038] The bakuchiol is purchased from Xi'an Shennong Biological Technology Co., Ltd.;

[0039] The vitamin C is purchased from Sumi Chemicals (Guangzhou) Co., Ltd.

[0040] Scutellaria extract ①

[0041] The preparation method comprises the following steps:

[0042] A1, taking dried scutellaria, crushing and passing through an 80-mesh sieve to obtain scutellaria powder;

[0043] A2, mixing the hydrogen bond acceptor and the hydrogen bond donor, heating and stirring to obtain a viscous liquid, adding ultrapure water and stirring uniformly to obtain a deep eutectic solvent with a water content of 30%;

[0044] A3, adding the Scutellaria powder into the deep eutectic solvent, performing pulsed direct current field treatment, centrifuging, filtering, and concentrating the supernatant to obtain the Scutellaria extract;

[0045] The hydrogen bond acceptor is matrine, the hydrogen bond donor is glycerol glucoside, and the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:3.

[0046] The temperature of heating and stirring in step A2 is 80℃, the rotating speed of heating and stirring is 550rpm, and the time of heating and stirring is 3h.

[0047] The ratio of the Scutellaria powder to the deep eutectic solvent in step A3 is 1g:25mL; the temperature of pulsed direct current field treatment is 35℃, and the time is 60min; the specific conditions of the pulsed direct current field are as follows: peak voltage 27V, pulse frequency 1.2kHz, and duty cycle 0.7.

[0048] Scutellaria extract 2

[0049] The preparation method comprises the following steps:

[0050] A1, taking dried Scutellaria, crushing, and passing through an 80-mesh sieve to obtain Scutellaria powder;

[0051] A2, mixing the hydrogen bond acceptor and the hydrogen bond donor, heating and stirring to obtain a viscous liquid, adding ultrapure water, and stirring uniformly to obtain a deep eutectic solvent with a water content of 40%;

[0052] A3, adding the Scutellaria powder into the deep eutectic solvent, performing pulsed direct current field treatment, centrifuging, filtering, and concentrating the supernatant to obtain the Scutellaria extract;

[0053] The hydrogen bond acceptor is matrine, the hydrogen bond donor is glycerol glucoside, and the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:1.5.

[0054] The temperature of heating and stirring in step A2 is 100℃, the rotating speed of heating and stirring is 400rpm, and the time of heating and stirring is 2h.

[0055] The ratio of the Scutellaria powder to the deep eutectic solvent in step A3 is 1g:40mL; the temperature of pulsed direct current field treatment is 25℃, and the time is 40min; the specific conditions of the pulsed direct current field are as follows: peak voltage 32V, pulse frequency 1.8kHz, and duty cycle 0.3.

[0056] Scutellaria extract 3

[0057] The preparation method comprises the following steps:

[0058] A1, taking dried Scutellaria, crushing, and passing through an 80-mesh sieve to obtain Scutellaria powder;

[0059] A2, mixing the hydrogen bond acceptor and the hydrogen bond donor, heating and stirring to obtain a viscous liquid, adding ultrapure water and stirring uniformly to obtain a deep eutectic solvent with a water content of 35%;

[0060] A3, adding the Scutellaria Baicalensis Georgi powder to the deep eutectic solvent, performing pulsed direct current field treatment, centrifuging, filtering and concentrating the supernatant to obtain the Scutellaria Baicalensis Georgi extract;

[0061] The hydrogen bond acceptor is matrine, the hydrogen bond donor is glycerol glucoside, and the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:2.2.

[0062] The heating and stirring temperature in step A2 is 90 DEG C, the heating and stirring speed is 500 rpm, and the heating and stirring time is 2.5 h.

[0063] The material liquid ratio of the Scutellaria Baicalensis Georgi powder to the deep eutectic solvent in step A3 is 1 g:32 mL, the pulsed direct current field treatment temperature is 30 DEG C, the treatment time is 50 min, the peak voltage of the pulsed direct current field is 30 V, the pulse frequency is 1.5 kHz, and the duty cycle is 0.5.

[0064] The Scutellaria Baicalensis Georgi extract 4 differs from the Scutellaria Baicalensis Georgi extract 3 only in that step A2 is omitted in the preparation of the Scutellaria Baicalensis Georgi extract 4, and a 75% ethanol solution is used instead of the deep eutectic solvent to treat the Scutellaria Baicalensis Georgi powder in step A3.

[0065] The Scutellaria Baicalensis Georgi extract 5 differs from the Scutellaria Baicalensis Georgi extract 3 only in that betaine is used instead of matrine as the hydrogen bond acceptor in the preparation of the Scutellaria Baicalensis Georgi extract 5, and other conditions are consistent with those of the Scutellaria Baicalensis Georgi extract 3.

[0066] The Scutellaria Baicalensis Georgi extract 6 differs from the Scutellaria Baicalensis Georgi extract 3 only in that panthenol is used instead of glycerol glucoside as the hydrogen bond donor in the preparation of the Scutellaria Baicalensis Georgi extract 6, and other conditions are consistent with those of the Scutellaria Baicalensis Georgi extract 3.

[0067] The Scutellaria Baicalensis Georgi extract 7 differs from the Scutellaria Baicalensis Georgi extract 3 only in that the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:1 in the preparation of the Scutellaria Baicalensis Georgi extract 7, and other conditions are consistent with those of the Scutellaria Baicalensis Georgi extract 3.

[0068] The Scutellaria Baicalensis Georgi extract 8 differs from the Scutellaria Baicalensis Georgi extract 3 only in that the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:3.5 in the preparation of the Scutellaria Baicalensis Georgi extract 8, and other conditions are consistent with those of the Scutellaria Baicalensis Georgi extract 3.

[0069] The component compositions (weight parts) of the whitening and anti-aging compositions of Examples 1-3 in the present application are shown in Table 1 below, and each component is weighed according to the formula amount, mixed and homogenized to obtain each composition.

[0070] Table 1 Components of the whitening anti-aging composition of Examples 1-3 and their weight fractions

[0071]

[0072] Comparative Example 1: Compared with Example 3, the difference is that no Scutellaria extract is added in Comparative Example 1, and the missing amount is made up of white pool flower seed oil, psoralen and vitamin C in a weight ratio of 11:7:5, and other conditions are consistent with Example 3.

[0073] Comparative Example 2: Compared with Example 3, the difference is that no white pool flower seed oil is added in Comparative Example 2, and the missing amount is made up of Scutellaria extract, psoralen and vitamin C in a weight ratio of 15:7:5, and other conditions are consistent with Example 3.

[0074] Comparative Example 3: Compared with Example 1, the difference is that no psoralen is added in Comparative Example 3, and the missing amount is made up of Scutellaria extract, white pool flower seed oil and vitamin C in a weight ratio of 15:11:5, and other conditions are consistent with Example 3.

[0075] Comparative Example 4: Compared with Example 1, the difference is that no vitamin C is added in Comparative Example 4, and the missing amount is made up of Scutellaria extract, white pool flower seed oil and psoralen in a weight ratio of 15:11:7, and other conditions are consistent with Example 3.

[0076] Comparative Example 5: Compared with Example 1, the difference is that Scutellaria extract ④ is used instead of Scutellaria extract ③ in Comparative Example 5, and other conditions are consistent with Example 3.

[0077] Comparative Example 6: Compared with Example 1, the difference is that Scutellaria extract ⑤ is used instead of Scutellaria extract ③ in Comparative Example 6, and other conditions are consistent with Example 3.

[0078] Comparative Example 7: Compared with Example 1, the difference is that Scutellaria extract ⑥ is used instead of Scutellaria extract ③ in Comparative Example 7, and other conditions are consistent with Example 3.

[0079] Comparative Example 8: Compared with Example 1, the difference is that Scutellaria extract ⑦ is used instead of Scutellaria extract ③ in Comparative Example 8, and other conditions are consistent with Example 3.

[0080] Comparative Example 9: Compared with Example 1, the difference is that Scutellaria extract ⑧ is used instead of Scutellaria extract ③ in Comparative Example 9, and other conditions are consistent with Example 3.

[0081] Test Example 1 Tyrosinase activity inhibition test

[0082] The synthesis of melanin is affected by tyrosinase, which can catalyze the hydroxylation of L-tyrosine into dopa and oxidize dopa into dopaquinone, and dopaquinone forms the final reaction product melanin. The test substance with inhibitory effect on tyrosinase activity can slow down the conversion of L-tyrosine into dopaquinone catalyzed by tyrosinase, and the inhibitory effect of the test substance on tyrosinase activity can be evaluated according to the change in absorbance at 475 nm by measuring the absorbance of dopaquinone at 475 nm.

[0083] The test example detects the inhibition rate of the compositions prepared in Examples 1-3 and Comparative Examples 1-9 of the present application on tyrosinase activity, so as to evaluate the inhibitory effect of the compositions on tyrosinase activity.

[0084] The specific test operation is as follows:

[0085] 1.1 Test substance treatment: the test substance (the composition prepared in Examples 1-3 and Comparative Examples 1-9) is respectively diluted with PBS buffer to a sample solution with a concentration of 80 mg / mL.

[0086] 1.2 Test grouping: in the 96-well enzyme-labeled plate, solvent background holes (T a ), solvent reaction holes (T b ), sample background holes (T c ), and sample reaction holes (T d ) are set. Among them, T a group is the solvent background group without adding substrate L-tyrosine solution and sample solution; T b group is the solvent reaction group with adding substrate L-tyrosine solution but without adding sample solution; T c group is the sample background group without adding substrate L-tyrosine solution but with adding sample solution; T d group is the sample reaction group with adding both substrate L-tyrosine solution and sample solution.

[0087] 1.3 Sample addition and determination: sample addition is carried out according to Table 2, and L-tyrosine solution, sample solution / solvent, and PBS buffer are sequentially added to each hole, fully mixed, incubated at 37°C for 10 min, and then 20 μL of tyrosinase solution is sequentially added to each hole, mixed at 37°C for 5 min, and then immediately placed into an enzyme-labeled instrument for determination at 475 nm wavelength.

[0088] Table 2 Sample addition table for tyrosinase activity inhibition test

[0089]

[0090] 1.4 The inhibition rate of tyrosinase activity is calculated according to the following formula:

[0091]

[0092] In the formula, Ad Absorbance of sample reaction well; A c Absorbance of sample blank well; A b Average absorbance of solvent reaction well; A a Average absorbance of solvent blank well; three replicates were made for each group, and the data were presented as the average value; the specific results are shown in Table 3.

[0093] Table 3 Tyrosinase inhibition rate data

[0094]

[0095] Test Example 2 Anti-aging effect test

[0096] External and body factors can cause the generation of free radicals. Free radicals have strong oxidizing ability. They attack any biomolecules through oxidation reaction, and cause various oxidative reactions with macromolecular substances such as lipids, carbohydrates, proteins, deoxyribonucleic acid, etc., resulting in denaturation, crosslinking, fragmentation and other oxidative damage, and further leading to the destruction of cell structure and function, and the damage of body tissues and organ lesions, which are manifested on the skin as aging, wrinkles, etc. Therefore, the DPPH free radical scavenging rate is one of the important indicators of anti-aging efficacy. The present test example evaluates the anti-aging performance of the compositions prepared in Examples 1-3 and Comparative Examples 1-9 of the present application by detecting the DPPH scavenging efficiency.

[0097] The specific test method is as follows:

[0098] 2.1 The test substances (the whitening and anti-aging compositions prepared in Examples 1-3 and Comparative Examples 1-9) were respectively diluted with anhydrous ethanol to a 2% concentration of the test solution; and a 0.2 mmol / L DPPH free radical solution was prepared with anhydrous ethanol;

[0099] 2.2 2 mL of the test solution was taken from each test sample group and placed in a test tube, and 2 mL of the aforementioned prepared DPPH free radical solution was added; 2 mL of deionized water was taken and placed in a test tube, and 2 mL of the aforementioned prepared DPPH free radical solution was added as a control group; 2 mL of the test solution was taken and placed in a test tube, and 2 mL of anhydrous ethanol was added as a blank group; each of the above groups was mixed well, and then reacted at room temperature for 30 min in the dark. The absorbance value was measured at 517 nm, and the scavenging rate was calculated according to the following formula:

[0100]

[0101] In the formula: A x Absorbance value of the test substance group; A0 is the absorbance value of the blank group; A1 is the absorbance value of the control group; each group was determined in triplicate, and the data were presented as the average value;

[0102] The test results are shown in Table 4.

[0103] Table 4 Anti-aging performance test results

[0104]

[0105]

[0106] Compared with Example 3, Comparative Examples 1-4 are respectively not adding one of Scutellaria extract, white pool flower seed oil, psoralen and vitamin C, and the preparation process of Scutellaria extract in Comparative Examples 5-9 is different from that in Example 3; wherein, ethanol is used instead of deep eutectic solvent for extraction in Comparative Example 5; different types of hydrogen bond acceptors and hydrogen bond donors are used in deep eutectic solvents of Comparative Examples 6-7; different molar ratios of hydrogen bond acceptors and hydrogen bond donors are used in deep eutectic solvents of Comparative Examples 8-9.

[0107] From the data of tyrosinase inhibition rate and DPPH clearance rate of Examples 1-3 and Comparative Examples 1-4 in Tables 3-4, it can be seen that Examples 1-3 which select Scutellaria extract, white pool flower seed oil, psoralen and vitamin C as four components have high tyrosinase inhibition rate and DPPH clearance rate, which indicates that the four components of Scutellaria extract, white pool flower seed oil, psoralen and vitamin C have synergistic effect, thereby improving the whitening and anti-aging ability of the composition.

[0108] From the data of tyrosinase inhibition rate and DPPH clearance rate of Example 3 and Comparative Examples 5-9 in Tables 3-4, it can be seen that the preparation process of Scutellaria extract will also significantly affect the extraction efficiency and effect of Scutellaria extract, thereby affecting the tyrosinase inhibition rate and DPPH clearance rate of the composition, that is, affecting the whitening and anti-aging effect of the composition.

[0109] In summary, the preparation method of Scutellaria extract provided by the present application, that is, the extraction method of deep eutectic solvent combined with pulse direct current treatment, has the advantages of low cost, high extraction efficiency, simple operation, non-toxicity, good biocompatibility, biodegradability and the like; the whitening and anti-aging composition provided by the present application is composed of Scutellaria extract, white pool flower seed oil, psoralen and vitamin C, has rapid and efficient whitening and anti-aging effect and is mild and non-irritating, and has wide application prospect.

[0110] Test Example 3 Red blood cell hemolysis test

[0111] Red blood cell hemolysis (RBC) experiment is one of the alternative methods of rabbit eye irritation experiment, and the basic principle is to evaluate the eye irritation of the test substance by measuring the amount of hemoglobin dissolved and the degree of denaturation;

[0112] Test substances: the whitening and anti-aging compositions prepared in Examples 1-3 of the present application, each of which was diluted with physiological saline to a mass fraction of 0.1%, 0.2%, 0.3%, 0.5%, 1%, 2%, 10% and 20% respectively for use;

[0113] The specific test procedure is as follows:

[0114] Take 500 μL of the prepared test substance and 500 μL of physiological saline containing 2% red blood cells, mix well, use physiological saline instead of the test substance for the negative control, and use physiological saline containing 1% SDS instead of the test substance for the positive control, set 3 parallels for each group, incubate in a 37°C biochemical incubator for 3 h, then centrifuge each group, take 10 μL of the supernatant and 90 μL of physiological saline, mix well in a 96-well plate, and measure the absorbance values at 410, 540 and 575 nm. Take the average value of each group, plot according to the hemolysis rate (HR), determine the regression equation, and calculate the test substance mass concentration HC 50 (mg / L) at which the test substance causes 50% hemolysis of red blood cells, and the DI value when the test substance mass fraction is 1%, and then calculate the L / D value of the test substance according to HC 50 and DI.

[0115] Among them, the hemolysis rate (HR), the hemoglobin denaturation rate (DI) and the L / D value are calculated according to the following formula:

[0116] Hemolysis rate (HR) = (A 受试物组410nm -A 阴性对照组410nm ) / (A 阳性对照组410nm -A 阴性对照组410nm ) × 100%;

[0117] Hemoglobin denaturation rate (DI) = (A 受试物组575nm -A 受试物组540nm ) / (A 阳性对照组575nm -A 阳性对照组540nm ) × 100%;

[0118] L / D = HC 50 / DI.

[0119] In the formula, A 受试物组410nm is the absorbance value at 410 nm of the test substance group; A 阴性对照组410nm is the absorbance value at 410 nm of the negative control group; A 阳性对照组410nm is the absorbance value at 410 nm of the positive control group; A 受试物组575nm is the absorbance value at 575 nm of the test substance group; A 阳性对照组575nm is the absorbance value at 575 nm of the positive control group; A 受试物组540nm is the absorbance value at 540 nm of the test substance group; and A 阳性对照组540nm is the absorbance value at 540 nm of the positive control group.

[0120] The red blood cell hemolytic stimulation is graded according to the value of L / D: L / D>100: no stimulation; 10

[0121] Table 5: Results of red blood cell hemolytic stimulation grading

[0122] Group L / D Irritation rating Example 1 ＞100 No irritation Example 2 ＞100 No irritation Example 3 ＞100 No irritation

[0123] As can be seen from the results of red blood cell hemolytic stimulation grading in Table 5, the L / D values of the whitening and anti-aging compositions prepared in Examples 1-3 are all greater than 100, indicating that the stimulation grades are all no stimulation, which shows that the compositions prepared in the application have the advantages of mildness and no stimulation.

[0124] Application Example: Whitening and Anti-aging Cream

[0125] The compositions of Examples 1-3 are added into the cream respectively to obtain the creams of Application Examples 1-3.

[0126] The whitening and anti-aging cream comprises the following components in mass percentage: the whitening and anti-aging composition 4%, isononyl isononanoate 2%, cetyl stearyl alcohol 2.5%, squalane 1.0%, dimethicone 2%, glycerol 4%, butylene glycol 7%, disodium EDTA 0.03%, carbomer 0.2%, p-hydroxyphenylacetone 0.5%, polyacrylamide 0.4%, fragrance 0.1%, arginine 0.18%, and deionized water in balance.

[0127] The preparation method of the whitening and anti-aging cream specifically comprises the following steps:

[0128] B1, isononyl isononanoate, cetyl stearyl alcohol, squalane and dimethicone are mixed and heated to 80℃, and then homogenized at a speed of 1000 rpm to obtain a phase a;

[0129] B2, glycerol, butylene glycol, disodium EDTA, carbomer and p-hydroxyphenylacetone are mixed and heated to 80℃, and then homogenized at a speed of 1000 rpm to obtain a phase b;

[0130] B3, the phase a and the phase b are mixed and homogenized at a speed of 1000 rpm to obtain a mixed solution;

[0131] B4, the mixed solution is cooled to 65℃, and then polyacrylamide, fragrance, arginine and deionized water are added, and homogenized at a speed of 1000 rpm, and then the whitening and anti-aging composition is added, stirred uniformly, cooled to 40℃, and discharged, to obtain the whitening and anti-aging cream.

[0132] Blank application example: compared with application examples 1-3, the difference lies in that the whitening and anti-aging composition is not added in the cream of the blank application example, and an equal amount of deionized water is used instead of the composition, and the preparation method is the same as that of application examples 1-3.

[0133] Test example 4: whitening and anti-aging human trial evaluation test

[0134] Twenty-four subjects (healthy, normal skin, no history of cosmetic allergy, aged 35-45 years) were selected, and the subjects were randomly divided into 4 groups, 6 people in each group. Under normal circumstances, the volunteers continuously used the product on the whole face for 28 days, and the whitening and anti-aging efficacy of application examples 1-3 and the blank application example was evaluated.

[0135] The specific steps are as follows:

[0136] The volunteers applied 2 g of cream to the face after cleansing and moisturizing in the morning and evening, and then massaged and absorbed it. The whole face was used once in the morning and once in the evening, and the volunteers were visited on the 0th and 28th days. On the day of the visit, the test area was washed with water without applying any product, and the volunteers sat in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 min. The improvement of facial aging indicators was quantified and photographed using instruments, including Cutometer 2mm MPA 580 to determine the skin plastic elasticity value (R2). The improvement before and after use was significantly different, and it was determined whether the sample had an elasticity effect; Skin-Colorimeter CL400 and Visia 7 (IPP) were used to determine the skin brightness, and the improvement before and after use was significantly different, and it was determined whether the sample had an improvement effect on skin brightness.

[0137]

[0138] In the formula: X 使用前 is the data of each indicator before use (0th day) of each group; X 使用后 is the data of each indicator after use (28th day) of each group;

[0139] The greater the brightness value, the more the color is biased towards white, indicating that the smaller the brightness value, the more the color is biased towards black. The skin plastic elasticity value R2 is used to represent the size of the skin's ability to recover after being stretched in the test. The greater R2, the younger the skin and the better the elasticity; on the contrary, the smaller R2, the older the skin and the worse the elasticity.

[0140] The specific statistical results are shown in Table 6. Compared with the blank application example, the skin plastic elasticity value and skin brightness of application examples 1-3 were significantly improved, indicating that the cream prepared from the composition provided by the technical scheme of the application can significantly improve the elasticity of facial skin and can significantly improve the skin color, and has excellent whitening and anti-aging efficacy.

[0141] Table 6: Whitening and anti-aging human trial evaluation test data

[0142]

[0143] The above description is only the preferred composition of the present application, and is not intended to limit the present application in any form. Although the present application has been disclosed as the preferred composition above, it is not intended to limit the present application. Any person skilled in the art can make some changes or modifications to the equivalent composition with the disclosed technical content above without departing from the technical solution of the present application. Any modification, equivalent change and modification to the above composition according to the technical essence of the present application without departing from the technical solution of the present application still belong to the scope of the technical solution of the present application.

Claims

1. A whitening and anti-aging composition comprising bakuchiol and vitamin C, characterized in that, By weight parts consisting of: Scutellaria extract 12-17 parts, white pool flower seed oil 9-13 parts, psoralen 5-9 parts, vitamin C 3-7 parts; The preparation method of the Scutellaria extract comprises the following steps: A1, taking dried Scutellaria, crushing, sieving, to obtain Scutellaria powder; A2, mixing the hydrogen bond acceptor and the hydrogen bond donor, heating and stirring to obtain a viscous liquid, adding ultrapure water, stirring uniformly to obtain a deep eutectic solvent with a water content of 30-40%; A3, adding the Scutellaria powder to the deep eutectic solvent, performing pulsed direct current field treatment, centrifuging, filtering, and concentrating the supernatant to obtain the Scutellaria extract; In step A2, the hydrogen bond acceptor is matrine, and the hydrogen bond donor is glycerol glucoside, and the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:1.5-3; the heating and stirring temperature is 80-100℃, the heating and stirring speed is 400-550 rpm, and the heating and stirring time is 2-3h; In step A3, the solid-liquid ratio of the Scutellaria powder to the deep eutectic solvent is 1g:25-40mL; the pulsed direct current field treatment temperature is 25-35℃, and the treatment time is 40-60min; the pulsed direct current field conditions are: peak voltage 27-32V, pulse frequency 1.2-1.8kHz, and duty cycle 0.3-0.

7.

2. The whitening anti-aging composition according to claim 1, wherein By weight parts consisting of: Scutellaria extract 12-17 parts, white pool flower seed oil 9-13 parts, psoralen 5-9 parts, vitamin C 3-7 parts; 3. The whitening anti-aging composition according to claim 1, wherein The molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is 1:2.

2.

4. The method for preparing the whitening and anti-aging composition according to any one of claims 1-3, characterized in that, The preparation method comprises the following steps: weighing each component according to the formula amount, mixing and homogenizing to obtain the whitening and anti-aging composition.

5. Use of the whitening and anti-aging composition of any one of claims 1-3 or the whitening and anti-aging composition prepared by the preparation method of claim 4 in the preparation of a skin care product with whitening and anti-aging effects.

6. The use according to claim 5, wherein the compound is ###0002### The skin care product is an essence, a jelly or a cream. The skin care product is an essence, a jelly or a cream.

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