Method for increasing nitrogen utilization efficiency of rape

A technology of rapeseed nitrogen and efficiency, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problem of combined research, there are few reports on the activity of tonoplast membrane proton pump and nitrogen utilization efficiency, there is no universal, effective methods, etc., to achieve the effect of reducing the content, improving the efficiency of nitrogen use, and efficient use of nitrogen

Active Publication Date: 2012-12-26
HUNAN AGRICULTURAL UNIV
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  • Summary
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AI Technical Summary

Problems solved by technology

[0005] It can be seen that there has been some research on improving plant nitrogen use efficiency at home and abroad, but so far there is no general and effective method, and there are few reports on the combination of tonoplast proton pump activity and nitrogen use efficiency.

Method used

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Embodiment Construction

[0014] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the following examples are used to describe the present invention in detail.

[0015] Cultivation of rapeseed seedlings: Soak the seeds of rapeseed varieties X-2 and X-6 in 20% (v / v) sodium hypochlorite for surface disinfection for 30 minutes, rinse with distilled water several times, and place them in a glass mesh room on September 30. For germination in soil culture, the composition of Hoagland complete nutrient solution used is 5 mmol / L KNO 3 , 1 mmol / L KH 2 PO 4 , 7 mmol / L MgSO 4 , 5 mmol / L Ca(NO 3 ) 2 4H 2 O, 3 mmol / L Fe-EDTA, 0.5 mg / L B, 0.5 mg / L Mn, 0.05 mg / L Zn, 0.02 mg / L Cu, 0.01 mg / L Mo, pour the nutrient solution every 5 days, 100 ml. The average day and night temperature in the glass mesh room is 29°C / 19°C, the natural humidity and light, and the light intensity is 205-230 Ue / (m 2 .s).

[0016] Transplanting of rapeseed se...

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Abstract

The invention discloses a method for increasing nitrogen utilization efficiency of rape, and provides a method for increasing the nitrogen utilization rate by reducing nitrate nitrogen content in rape plants. The method comprises the following steps of: culturing rape seedlings in a glass net chamber by using Hoagland complete nutrient solution (the nutrient solution comprises 5 mmol / L of KNO3, 1 mmol / L of KH2PO4, 7 mmol / L of MgSO4, 5 mmol / L of Ca(NO3)2.4H2O, 3 mmol / L of Fe-EDTA, 0.5 mmol / L of B, 0.5 mmol / L of Mn, 0.05 mmol / L of Zn, 0.02 mmol / L of Cu and 0.01 mmol / L of Mo) through soil culture; seeding in September in the same year; transplanting in a big field after one month; complementarily applying 5 kg of urea for every mu after transplanting and reviving at the seedling stage; irrigating at a proper stage and dressing bolting flower fertilizer; dressing 7.5 kg of budding bolting fertilizer urea per mu in coordination with spring irrigation in spring; and uniformly spraying inhibitor solution simultaneously containing 50 mmol / L of Bafilomycin A1 and 20 mumol / L of DCCD (N, N'-dicyclohexylcarbodiimide) at the beginning of florescence of rape once every six days until a pod development stage begins. The content of nitrate nitrogen in the plants can be obviously reduced, and further the nitrogen utilization efficiency of the rape plants is obviously increased.

Description

technical field [0001] The invention relates to a plant cultivation method, in particular to a method for reducing the nitrate nitrogen content of rapeseed plants so as to improve nitrogen utilization efficiency. Background technique [0002] my country's annual nitrogen fertilizer consumption is 25 million tons (pure nitrogen), ranking first in the world, but the rate of diminishing returns on nitrogen fertilizers is becoming more and more serious. In many places, nitrogen fertilizers have increased, and yields have stopped increasing or even decreased, seriously violating low-carbon economic requirements. In addition, the increase of nitrogen fertilizer has made the nitrate content in crops soar, which has become one of the main factors restricting the foreign exchange earning of my country's agricultural products export. Therefore, it is one of the hotspots in the research of plant nutritional efficiency in recent years to improve the nitrogen use efficiency from the plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01C1/08A01C1/00A01G7/06
Inventor 张振华宋海星刘强荣湘民彭建伟谢桂先张玉平
Owner HUNAN AGRICULTURAL UNIV
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