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A molecular marker and lotus mist technology, which is applied in the direction of DNA/RNA fragments, microbial measurement/inspection, recombinant DNA technology, etc., can solve the difficulty of collection, preservation, identification and utilization of lotus mist germplasm resources, and restrict the breeding of excellent varieties and promotional applications
Active Publication Date: 2017-07-04
永春县产品质量检验所福建省香产品质量检验中心国家燃香类产品质量监督检验中心福建
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Since the lotus mist was introduced into my country from Java, Malaysia and other countries, many local varieties and resources have been produced under long-term adaptive cultivation. These local varieties are mostly named after the color of the fruit, such as 'Dahonglianwu', 'P
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[0018] 1 Materials and methods
[0019] 1.1 Transcriptome data sources
[0020] The lotus mist transcriptome data comes from the results of Illumina high-throughput deep sequencing of the fruit in 2016 by our research group. During the sequencing, the fruits of three varieties of Lianwu, namely 'Zihong', 'Black Pearl' and 'Green Diamond', were collected. For each variety, 20 Lianwu fruits from 3 different individual plants were collected, quick-frozen in liquid nitrogen, and stored at -80°C. . When sequencing, construct 1 library for each individual plant. Sequencing commissioned Beijing Biomike Biotechnology Co., Ltd. to use Illumina HiSeqTM 2500 PE125 system for RNA-Seq transcriptome sequencing (no reference), and Trinity was used for sequence assembly, and 7.80G of effective data was obtained after filtering.
[0021] 1.2 Materials and DNA extraction
[0022] The materials used for SSR primer screening and usability evaluation were 30 germplasm resources from the Lianwu...
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Abstract
The invention provides a syzygium samarangense EST-SSR molecular marker, and belongs to the field of a molecular marker. By utilizing syzygium samarangense transcriptome data to carry out SSR marker development, a high-frequency SSR site can be obtained; 22 pairs of primers are determined; types are rich; richer and more reliable marker selection is provided for syzygium samarangense genetic diversity analysis and genetic map construction.
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technical field [0001] The invention belongs to the field of molecular markers, in particular to lotus mist EST-SSR molecular markers. Background technique [0002] wax apple[ Syzygium samarangense (Blume) Merri.et Perry] native to the Malay Peninsula and the Andaman Islands, is Myrtaceae (Myrtaceae) rose fruit ( Syzygium ) Tropical evergreen fruit trees, also known as foreign apples, South Sea apples, Java apples, sweet mist, water pomegranate and so on. Lotus mist is rich in a variety of medicinal ingredients, such as flavonoids, chalcones, hexane, etc., which have antidiarrheal, analgesic, antibacterial, anti-hyperglycemic, anti-cholinesterase, anti-inflammatory, and immune-enhancing effects . Lianwu fruit has bright color, unique shape, sweet and refreshing taste, good taste, and rich in phenolic substances. It is one of the fruits with great health value and is loved by consumers at home and abroad. It is widely planted in Malaysia, Thailand, Indonesia and other p...
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