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Molecular marker related to pig muscle pH value character and application thereof

A molecular marker, muscle technology, applied in the application, microbial determination/inspection, biochemical equipment and methods, etc., can solve problems such as the relationship between SNP polymorphism of porcine GYS1 gene promoter and pork quality traits that have not been studied.

Inactive Publication Date: 2015-02-25
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is still no relevant report on the study of the relationship between the SNP polymorphism of the pig GYS1 gene promoter and the quality traits of pork

Method used

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  • Molecular marker related to pig muscle pH value character and application thereof
  • Molecular marker related to pig muscle pH value character and application thereof
  • Molecular marker related to pig muscle pH value character and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: (1) Amplification of the DNA sequence of the promoter region of the pig GYS1 gene

[0032] (1) Primer design:

[0033] Using the cDNA of the porcine GYS1 gene (GenBank accession number: GQ845034) as an information probe, the homologous sequence screening was performed in the Genomic Biology porcine genome database using the BLAST tool of the National Center for Biotechnology Information (NCBI). Obtain a BAC clone CH242-517N5 (GenBank accession number: FP102974) containing the pig GYS1 gene, use the sequence of the BAC clone as a template to design primers, and use the genomic DNA of the Chinese local pig breed Meishan pig as a template to obtain the pig GYS1 gene promoter sequence. A pair of primers were designed with Primer Premier 5.0 software to amplify the sequence described in SEQ ID NO: 1, and their sequence size is 785bp respectively.

[0034] The DNA sequence of the 1 pair of primers is as follows:

[0035] SNP-1F: 5'-ATCCTAGCGGAAACGGAA-3'

[0036...

Embodiment 2

[0050] Example 2: Polymorphic distribution of molecular markers in different pig populations

[0051] Polymorphisms with two mutations in three local breeds (48 Meishan pigs, 21 Tongcheng pigs and 22 Qingping pigs) and three exotic breeds (32 Large White, 35 Changbai and 28 Duroc) Distribution detection, the results are shown in Table 1, the Mva?-RFLP polymorphism distribution in the GYS1 promoter region, the T allele dominates in local varieties, and the C allele dominates in foreign varieties. There are also significant differences (Table 1).

[0052] Table 1 Different pig breeds GYS1 Gene wxya Genotype and gene frequency of polymorphism

[0053]

Embodiment 3

[0054] Example 3: Application of Molecular Markers in Meat Quality Traits

[0055] analysis pig GYS1 Gene wxya - Association analysis and genetic effect estimation of different genotypes of PFLP and meat quality traits. The groups used for statistical analysis include two batches of Da×Mei F2 resource families in 2000 and 2003. The meat quality traits analyzed mainly include intramuscular fat content, water retention, water loss rate, pH value of longissimus dorsi muscle 45 minutes after slaughter, pH value of biceps femoris, pH value of semimembranosus muscle, color value of longissimus dorsi muscle, color value of biceps femoris muscle and marbling score. The applicant used the SAS statistical software (SAS Institute Inc, Version 8.0) glm program to conduct single-marker analysis of variance and significant test. The model used was:

[0056] Yijkl=μ+Gi +Sk+Yl(+bijklXijkl)+eijkl

[0057] Yij is the trait phenotype value, μ is the average value, Gi is the genotype effect...

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Abstract

The invention belongs to the technical field of preparation of a pig genetic marker, and particularly relates to a preparation method of a pig muscle pH value related molecular marker and application of the pig muscle pH value related molecular marker on auxiliary marker selection. The molecular marker is obtained by cloning a GYS1 gene promoter sequence, and the nucleotide sequence of the molecular marker is as shown in the sequence table SEQ ID NO: 1; and a C267-T267 basic group on the 267th basic group of a sequence as shown in the sequence table SEQ ID NO: 2 is mutated to cause MvaIPCR-RFLP polymorphism. The invention also discloses application of the preparation method for obtaining the molecular marker and a polymorphism detection method. The preparation method disclosed by the invention provides a new genetic marker for carrying out the auxiliary marker selection of a high-muscle quality pig.

Description

technical field [0001] The invention belongs to the technical field of preparation of animal molecular markers, and in particular relates to the cloning of a promoter fragment of a gene GYS1 related to pig muscle pH value traits, the preparation of molecular markers and its application in marker-assisted selection. Background technique [0002] The content of glycogen in animals directly affects some quality traits of muscles, such as the pH value of muscles after slaughter, water retention, and muscle tenderness. The protein breakdown and glycogen synthesis processes of skeletal muscle are directly related to muscle quality. Therefore, the research on the process of glycogen synthesis and catabolism in skeletal muscle has always attracted people's attention. The final pH value of the muscle after slaughter is an important indicator to measure the quality of pig muscle. If the pH value of the muscle is too high, it is easy to form DFD meat, and if the pH value of the muscle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/54C12N15/10C12Q1/68
Inventor 王林杰王薏琳王艳李利张红平仲涛
Owner SICHUAN AGRI UNIV
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