Chicken dominant white feather gene identification

A dominant and genetic technology, applied in the field of animal genetics and breeding, can solve the problems of inapplicability, poor stability, and long time consumption, and achieve the effects of rapid detection, accurate results, and simple operation

Active Publication Date: 2015-06-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the methods reported so far to detect the dominant white feather gene, whether it is to design primers before and after the PMEL17 gene mutation site and then amplify it, and then identify it by PAGE method; The detection of mutation sites has problems such as long time-consuming and poor stability, and is not suitable for the detection of a large number of samples in breeding.

Method used

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  • Chicken dominant white feather gene identification
  • Chicken dominant white feather gene identification
  • Chicken dominant white feather gene identification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Preparation of a kit for identifying the genotype of the chicken dominant white feather gene and its method of use

[0042] One, be used for identifying the design of the primer pair of the genotype of chicken dominant white feather gene

[0043] According to the upstream and downstream sequences of the 9bp insertion mutation on the 10th exon of the dominant white chicken PMEL17 gene sequence, the following primer pairs were designed:

[0044] Upstream primer: 5'-AACGGCAACGGCTTGGCTGTGG-3' (sequence 3, which is the 1-22th position of sequence 1 and sequence 2);

[0045] Downstream primer: 5'-CCGGTAGGTGTAGGCAGCGGTG-3' (sequence 4, which is the reverse complementary sequence of the last 22 positions of sequence 1 and sequence 2).

[0046] When there is no 9bp insertion mutation in the exon 10 of the PMEL17 gene sequence of the genomic DNA of the chicken to be tested, the length of the amplified target fragment is 217bp (sequence 1); When there is a 9bp inserti...

Embodiment 2

[0066] Embodiment 2, utilize the kit prepared in embodiment 1 to identify the genotype of chicken dominant white feather gene

[0067] 1. Identification of Chicken Dominant White Feather Gene of Known Genotype

[0068] 1. Use the following chickens with known genotypes as the chickens to be tested:

[0069] 1 white legion chicken (homozygous for dominant white feathers, genotype II, phenotype is white), 2 silky chickens (homozygous for non-dominant white feathers, genotype ii, phenotype is white feathers, belonging to recessive white feathers) ), and 2 F1 hybrids of silky silkies and white leghorns (heterozygous for dominant white feathers, genotype Ii, phenotype of white feathers). The genotypes of the above chickens have been verified by pure breeding and hybridization, and the genotypes have been determined.

[0070] Using the kit prepared in Example 1, the genotype identification of the dominant white feather gene was carried out for each chicken to be tested according t...

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PUM

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Abstract

The present invention discloses a chicken dominant white feather gene identification. The present invention provides primers for identifying the chicken dominant white feather gene, specifically the primer pairs comprising two single-stranded DNA represented by a sequence 3 and a sequence 4 in the sequence table. Experiment results show that: with the application of the method of the present invention to identify the genotype (genotype II or genotype Ii or genotype ii) of the chicken dominant white feather gene, the advantages of simple operation, rapid detection, accurate result, low toxicity, broad application range and the like are provided. According to the present invention, the important significance is provided for the white feather chicken breeding, and the white feather character can be easily and effectively selected during the breeding practice so as to guide the molecular genetic marker selection of the chicken dominant white feather character.

Description

technical field [0001] The invention belongs to the field of animal genetics and breeding, and relates to a method for identifying chicken dominant white feather genes. Background technique [0002] Feather color phenotype is a complex quality trait regulated by multiple genes. There are many related studies on feather color traits, but there are few related gene functions and mutations. Only a small number of genes have been confirmed to be related to the formation of specific feather colors. [0003] The feather color of chickens can be divided into white feathers and colored feathers. The white feathers of chickens are divided into two categories: dominant white feathers and recessive white feathers. Among them, dominant white-feathered chickens are represented by Bailaihang chickens, recessive white-feathered chickens such as silky chickens and various recessive white chickens, and colored feather chickens such as Shouguang chickens. [0004] The dominant white feathe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 邓学梅赵妤娟张熙悦
Owner CHINA AGRI UNIV
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