Marker primer interlocked with wheat powdery mildew resistance gene PmHNK54 and application thereof

Abstract

The invention relates to a marker primer interlocked with a wheat powdery mildew resistance gene PmHNK54 and application thereof in molecular marker-assisted selection. The sequence of the marker primer on a genetic map is Xbarc5, PnHNK54 and Xgwm312, and the genetic distances between Xbarc5 and PnHNK54 and between Xgwm312 and PnHNK54 are respectively 5.0cM and 6.0cM. 10 mu l of PCR (Polymerase Chain Reaction) reaction system during SSR (Simple Sequence Repeat) marker selection comprises 1 mu l of 10*buffer, 0.2 mu L of 10nM / mu l dNTP, 0.2 mu l of 20u M / mu L primer, 0.1 mu l of 5U / mu lTaq enzyme, 8.1 mu l of deionized water and 0.4 mu l of 20 ng / mu l genome DNA. An amplification procedure comprises the following steps of: pre-denaturing at 94 DEG C for 3 min, denaturing at 94 DEG C for 1 min, renaturing at 55 or 60 DEG C for 1 min, extending at 72 DEG C for 2 min, and repeating for 40 cycles; extending at 72 DEG C for 10 min; and performing 6% denaturing polyacrylamide gel electrophoresis or 8% nondenaturing polyacrylamide gel electrophoresis on amplification products, observing and taking pictures after silver nitrate dyeing. The invention finds the position of the powdery mildew resistance gene PmHNK54 in wheat parent material Zheng 9754, has the advantages of strong marker specificity, high stability, cost saving and high selection efficiency and is applicable to large scale, high throughput and automation.

Description

technical field [0001] The invention relates to a marker primer for a wheat powdery mildew resistance gene, which belongs to the field of molecular genetics, and in particular relates to a wheat powdery mildew resistance gene PmHNK54 Tightly linked marker primers and their application in molecular marker-assisted breeding. Background technique: [0002] Wheat powdery mildew is caused by the wheat powdery mildew [ Blumeria graminis (DC) Speer f. sp. Tritici ,Referred to as Bgt ] is an airborne fungal disease caused by wheat, which is one of the main diseases in the world's major wheat regions, and the scope of the disease is expanding day by day, and the degree of damage is increasing, which can cause a yield loss of 13.4% to 34%. Breeding and using high-efficiency disease-resistant varieties is the most economical, safe and effective solution. According to the "gene-to-gene" hypothesis, any disease-resistant gene will produce a virulent race that overcomes its re...

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Problems solved by technology

However, this type of resistance source is often linked with unfavorable agronomic traits. When the resistance source is introduced into a cultivar, the offspring need to go through multiple generations of backcrossing to break the linkage with the unfavorable gene, and some even cannot break the linkage after multiple generations of backcrossing. linkage, which largely restricts the application of such sources of resistance in breeding

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