Molecular marker for soybean bemisia tabaci resistance breeding and marking method of molecular marker

A molecular marker, Bemisia tabaci technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. Gene has not been reported and other problems, to achieve the effect of reducing the workload of high-generation breeding selection, reducing the workload of selection, and realizing fine positioning

Inactive Publication Date: 2018-06-12
CROP RES INST SHANDONG ACAD OF AGRI SCI
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  • Claims
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AI Technical Summary

Problems solved by technology

Bemisia tabaci resistant varieties have been applied to a certain extent in cotton and tomato breeding, but there are few studies on the molecular mechanism of bemisia tabaci resistance, and there are no reports on the resistance genes of bemisia tabaci

Method used

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  • Molecular marker for soybean bemisia tabaci resistance breeding and marking method of molecular marker
  • Molecular marker for soybean bemisia tabaci resistance breeding and marking method of molecular marker
  • Molecular marker for soybean bemisia tabaci resistance breeding and marking method of molecular marker

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Embodiment 1，1

[0019] Embodiment 1, 1. a kind of molecular marker method that is used for soybean anti-bemisia tabaci breeding, comprises the following steps:

[0020] 1) Distribution of soybean RIL populations resistant to whitefly: Huapidou and Qihuang 26 high-generation RIL populations were planted for two consecutive years, and soybean RIL populations were identified for resistance to whitefly, repeated twice; Analyze the average;

[0021] 2) QTL mapping of soybean resistance to whitefly: using the high-density genetic map constructed by SLAF-seq technology to map the QTL of soybean RIL population resistance to whitefly, and obtain two QTLs related to soybean resistance to whitefly location.

[0022] 2. Identification of Bemisia tabaci resistance in soybean RIL population

[0023] In 2016, 149 soybean RIL populations were identified for resistance to whitefly, repeated twice. Analysis of the 2 averages showed that (Table 1, figure 1 ): The resistance of RIL populations to Bemisia tab...

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Abstract

The invention relates to a molecular marking method for soybean bemisia tabaci resistance breeding. The molecular marking method comprises the following steps of 1, distribution of resistances to bemisia tabaci by soybean RIL groups, wherein high generation RIL groups such as huapidou and qihuang 26 are planted for 2 years continuously, the resistances to bemisia tabaci by the soybean RIL groups are identified, and the operation is repeated twice; the quadratic mean is analyzed; 2, QTL locating of the resistances to bemisia tabaci by soybeans, wherein a high-density genetic map built through an SLAF-seq technology is utilized to conduct QTL locating on the resistances to bemisia tabaci by the soybean RIL groups to obtain two QTL loci related to the resistances to bemisia tabaci by the soybeans. Through the marking combination, a breeding material is subjected to marking and selecting of the low generations, superior genes of the resistances to bemisia tabaci in the breeding material are easily aggregated in the low generations, the workload of breeding selection of high generations is lowered, the breeding scale is reduced, and the breeding age limit is shortened.

Description

technical field [0001] The invention relates to the technical field of molecular marker-assisted breeding, in particular to a molecular marker and a marking method thereof for soybean breeding against whitefly. Background technique [0002] Soybean is an important grain, oil and feed crop in my country, and has an important strategic position in the development of the national economy. At present, the crops damaged by bemisia tabaci are as high as 100 million mu in my country every year, and soybean is one of the most seriously damaged crops. The vast area from Hainan Island in the south to Heilongjiang in the north, Heilongjiang in the east and Xinjiang in the west is all affected. The harm of bemisia tabaci is increasing year by year. [0003] Chemical control is a more effective way to control general agricultural pests, but due to the biological advantages of Bemisia tabaci, such as strong migratory ability, miscellaneous feeding habits, waxy protective layer, strong rep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张彦威李伟林延慧张礼凤王彩洁徐冉
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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