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Molecular marker method relevant to plant heights of soy beans and marker combination thereof

A molecular marker, high correlation technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as inability to assist selective breeding, and achieve reduction of high-generation breeding selection workload, short physical intervals, and narrow breeding. effect of scale

Inactive Publication Date: 2018-08-10
CROP RES INST SHANDONG ACAD OF AGRI SCI
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Problems solved by technology

However, soybean plant height is a quantitative trait controlled by multiple genes, and the plant height QTL is material-specific and cannot be used in molecular marker-assisted selection breeding for specific breeding populations.

Method used

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  • Molecular marker method relevant to plant heights of soy beans and marker combination thereof
  • Molecular marker method relevant to plant heights of soy beans and marker combination thereof
  • Molecular marker method relevant to plant heights of soy beans and marker combination thereof

Examples

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Effect test

Embodiment 1

[0020] Embodiment 1, a kind of molecular marker method related to soybean plant height, comprises the following steps:

[0021] 1) Plant height distribution of soybean RIL populations: The high-generation RIL populations of Huapidou and Qihuang 26 were planted for two consecutive years, and the plant heights were counted. It was found that the plant height traits of the RIL populations basically showed a continuous distribution. Such as Figure 1-2 shown.

[0022] 2) QTL mapping of soybean plant height: The high-density genetic map constructed based on SLAF-seq technology was used to map the QTL of plant height in soybean RIL population, and a total of 9 soybean plant height-related QTLs were obtained by using complete interval mapping (ICIM) site ( image 3 ), located on chromosomes 1, 5, 6, 7, 8, 8, 16, 17 and 19 (Table 1), explaining 93.32% of the phenotypic variation. Such as image 3 shown.

[0023] Table 1 QTL loci related to soybean plant height

[0024]

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Abstract

The invention relates to a molecular marker method relevant to the plant heights of soy beans. The molecular marker method comprises the following steps of 1), distributions of the plant heights of RIL (Recombinant Inbred Line) populations of the soy beans: continuously planting advanced-generation RIL populations of Huapi bean and Qihuang bean for two years, and counting the plant heights of theadvanced-generation RIL populations; 2), QTL (Quantitative Trait Locus) location of the plant heights of the soy beans: carrying out the QTL location on the plant heights of the RIL populations of thesoy beans by a high-density genetic map constructed by utilizing an SLAF-seq (Specific Locus Amplified Fragment-sequencing) technique, to obtain nine QTL sites relevant to the plant heights of the soy beans. The low-generation marker selection is carried out on a breeding material by utilizing a marker combination provided by the invention; the aggregation on plant-height-excellent genes in the breeding material in a low generation is facilitated; the workload of advanced-generation breeding selection is reduced; the breeding scale is reduced, and the breeding age limit is shortened.

Description

technical field [0001] The invention relates to the technical field of molecular marker-assisted breeding, in particular to a soybean plant height-related molecular marker method and marker combination thereof. Background technique [0002] Soybean is an important grain, oil and feed crop in my country, and has an important strategic position in the development of the national economy. Plant height is an important factor of soybean plant type, which directly affects the planting density, lodging resistance and yield of soybean. Soybean plant height is also an important ecological trait. Under specific ecological conditions, only by selecting the appropriate plant height type can the yield be improved. At present, there are 196 QTLs related to soybean plant height included in the SoyBase database. However, soybean plant height is a quantitative trait controlled by multiple genes, and plant height QTLs are material-specific and cannot be used in molecular marker-assisted sel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13
Inventor 张彦威李伟林延慧张礼凤王彩洁徐冉
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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