Molecular markers, screening methods, breeding methods and applications for screening soybean materials

Abstract

The invention discloses a molecular marker for screening soybean materials, a screening method, a breeding method and applications. The molecular markers include InDel markers or / and dCAPS markers. The step of using the aforementioned molecular markers to screen soybean high-protein materials includes: selecting the InDel Mark as a primer pair; use the InDel-marked primer pair to select one or more soybean variety DNAs for first PCR amplification; perform the first electrophoresis detection on the first PCR amplification product, and the first electrophoresis detection band The soybean varieties presented as high bands are low-protein varieties, and the soybean varieties presented as low bands in the first electrophoresis detection band are high-protein varieties. Based on the whole genome resequencing and QTL positioning technology, the present invention develops high-protein InDel and dCAPS with clear targets, and uses the combination of InDel and dCAPS to mark soybean high-protein resources in the future and molecular marker-assisted breeding for high-protein, agronomic and Breeding materials or varieties with excellent quality traits provide new ways.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and relates to a molecular marker for screening soybean high-protein materials, a method for screening soybean high-protein materials using the primers, and a method for using the primers to screen target strains for breeding. Background technique [0002] Soybean grains are rich in protein, accounting for 31% to 55% of grain dry matter, and are one of the main sources of human food protein. Its protein output accounts for two-thirds of the protein output of protein crops, and what is even more rare is that it is rich in a variety of essential amino acids. The World Health Organization defines soybean protein as a first-class protein. At the same time, soybean protein has good functional properties and can be used in a variety of food systems to improve product quality. [0003] At present, protein-related QTL loci have been found on all 20 chromosomes of soybean, among which 213 protei...

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