Four-primer molecular marking method for identifying different genotypes of rice grain length gene GS3

Abstract

The invention relates to a four-primer molecular marking method for identifying different genotypes of a rice grain length gene GS3, belonging to the technical field of agricultural bioengineering. Four marking primers are designed and synthesized according to differences of a long and large-grain variety TD70 and a short and small-grain variety Kasalath in the nucleotide base sequence of the grain length gene GS3, and are added into the same PCR system for amplifying different rice DNA so as to distinguish different genotypes of homozygotes and heterozygotes of the GS3. The method disclosed by the invention not only can be used for rapidly and accurately identifying the different genotypes of the grain length gene GS3 in a rice genetic resource or a breeding population but also can be used for improving the selection efficiency on a long-grain genotype GS3-TGS3-T and speeding up the breeding progress of a new long-grain, high-quality and high-yield rice variety.

Description

1. Technical field [0001] The invention relates to a four-primer molecular marking method for identifying different genotypes of rice grain length gene GS3, which belongs to the field of biotechnology engineering and is specially used for T GS3- T Screening, identification and marker-assisted breeding of genotyped long-grain, high-quality, high-yielding rice resources. 2. Background technology [0002] my country is a big country in rice production and consumption, and more than 60% of the population in the country takes rice as a staple food. In recent years, with the improvement of people's living standards and the intensification of domestic and foreign rice market competition, high-quality rice has begun to show broad market prospects and is favored by more and more consumers. Therefore, on the basis of ensuring the improvement of rice yield per unit area, improving rice quality has become an important issue in rice breeding (Yi Junliang et al., Molecular Plant Breedin...

AI Technical Summary

Problems solved by technology

[0007] Technical problem: the present invention aims at the defects such as poor accuracy, cumbersome test procedures and high cost in the process of using linked microsatellite markers and CAPS markers to distinguish different genotypes of the grain length gene GS3. For the single nucleotide base mutation from C to A in the exon, design four gene-specific primers and use a one-step PCR method to quickly and accurately identify different genotypes of GS3, so as to screen long-grain rice germplasm resources , Identify and mark the purpose of assisted breeding, and at the same time improve the selection efficiency of grain length increasing genotypes, and accelerate the process of breeding long-grain high-quality, high-yielding rice varieties

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