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Four-primer molecular marking method for identifying different genotypes of rice grain length gene GS3

A genotype, GS3-O-F technology, applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of cumbersome test procedures, high cost, poor accuracy, etc., and improve selection efficiency , Reduce breeding costs and shorten the breeding cycle

Active Publication Date: 2015-04-29
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology

[0007] Technical problem: the present invention aims at the defects such as poor accuracy, cumbersome test procedures and high cost in the process of using linked microsatellite markers and CAPS markers to distinguish different genotypes of the grain length gene GS3. For the single nucleotide base mutation from C to A in the exon, design four gene-specific primers and use a one-step PCR method to quickly and accurately identify different genotypes of GS3, so as to screen long-grain rice germplasm resources , Identify and mark the purpose of assisted breeding, and at the same time improve the selection efficiency of grain length increasing genotypes, and accelerate the process of breeding long-grain high-quality, high-yielding rice varieties

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  • Four-primer molecular marking method for identifying different genotypes of rice grain length gene GS3
  • Four-primer molecular marking method for identifying different genotypes of rice grain length gene GS3
  • Four-primer molecular marking method for identifying different genotypes of rice grain length gene GS3

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Embodiment Construction

[0036] Through the identification and analysis of grain shape traits of more than 3,000 rice germplasm resources stored at low temperature in the medium-term bank of agricultural germplasm resources in Jiangsu Province, one was screened from Swan Valley / / / 9520 / / (72-496 / 御 Waxy) long and large-grain japonica rice stable line TD70. Using TD70 to cross the short and small-grain Indian indica rice variety Kasalath, a recombinant inbred line population containing 240 lines was constructed by single-seed transmission method. Through the drawing of molecular linkage maps and the QTL mapping analysis for two consecutive years from 2010 to 2011, in rice Multiple QTLs related to grain type were mapped on the chromosome, and one grain length QTL-qGL3-1 was mapped on chromosome 3. Through the sequencing analysis of TD70 and Kasalath genomic DNA, it was determined that qGL3-1 is the reported GS3 gene, a major QTL controlling grain length traits located near the centromere of chromosome 3 in ...

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Abstract

The invention relates to a four-primer molecular marking method for identifying different genotypes of a rice grain length gene GS3, belonging to the technical field of agricultural bioengineering. Four marking primers are designed and synthesized according to differences of a long and large-grain variety TD70 and a short and small-grain variety Kasalath in the nucleotide base sequence of the grain length gene GS3, and are added into the same PCR system for amplifying different rice DNA so as to distinguish different genotypes of homozygotes and heterozygotes of the GS3. The method disclosed by the invention not only can be used for rapidly and accurately identifying the different genotypes of the grain length gene GS3 in a rice genetic resource or a breeding population but also can be used for improving the selection efficiency on a long-grain genotype GS3-TGS3-T and speeding up the breeding progress of a new long-grain, high-quality and high-yield rice variety.

Description

1. Technical field [0001] The invention relates to a four-primer molecular marking method for identifying different genotypes of rice grain length gene GS3, which belongs to the field of biotechnology engineering and is specially used for T GS3- T Screening, identification and marker-assisted breeding of genotyped long-grain, high-quality, high-yielding rice resources. 2. Background technology [0002] my country is a big country in rice production and consumption, and more than 60% of the population in the country takes rice as a staple food. In recent years, with the improvement of people's living standards and the intensification of domestic and foreign rice market competition, high-quality rice has begun to show broad market prospects and is favored by more and more consumers. Therefore, on the basis of ensuring the improvement of rice yield per unit area, improving rice quality has become an important issue in rice breeding (Yi Junliang et al., Molecular Plant Breedin...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q2531/113
Inventor 陈涛姚姝丁丹郑佳王才林张亚东朱镇赵庆勇周丽慧赵凌于新赵春芳
Owner JIANGSU ACAD OF AGRI SCI
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