Four-primer molecular marking method for identifying different genotypes of rice grain length gene GS3
A genotype, GS3-O-F technology, applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of cumbersome test procedures, high cost, poor accuracy, etc., and improve selection efficiency , Reduce breeding costs and shorten the breeding cycle
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[0036] Through the identification and analysis of grain shape traits of more than 3,000 rice germplasm resources stored at low temperature in the medium-term bank of agricultural germplasm resources in Jiangsu Province, one was screened from Swan Valley / / / 9520 / / (72-496 / 御 Waxy) long and large-grain japonica rice stable line TD70. Using TD70 to cross the short and small-grain Indian indica rice variety Kasalath, a recombinant inbred line population containing 240 lines was constructed by single-seed transmission method. Through the drawing of molecular linkage maps and the QTL mapping analysis for two consecutive years from 2010 to 2011, in rice Multiple QTLs related to grain type were mapped on the chromosome, and one grain length QTL-qGL3-1 was mapped on chromosome 3. Through the sequencing analysis of TD70 and Kasalath genomic DNA, it was determined that qGL3-1 is the reported GS3 gene, a major QTL controlling grain length traits located near the centromere of chromosome 3 in ...
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