A kasp molecular marker method for identifying rice blast resistance gene pigm

A technology of molecular markers and resistance genes, applied in the field of biotechnology engineering, can solve the problems of time-consuming and labor-intensive breeding, low accuracy, cumbersome detection process, etc., and achieve the effects of shortening the breeding cycle, reducing breeding costs, and improving selection efficiency

Active Publication Date: 2022-07-08
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology

[0007] Technical problem: the present invention aims at the time-consuming and labor-intensive breeding of rice blast resistance with the traditional Pigm gene and is highly susceptible to environmental interference and the accuracy is not high, and the existing molecular markers have poor polymorphism in different parents, the accuracy is not high, and the detection process is relative cumbersome and other disadvantages

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  • A kasp molecular marker method for identifying rice blast resistance gene pigm
  • A kasp molecular marker method for identifying rice blast resistance gene pigm
  • A kasp molecular marker method for identifying rice blast resistance gene pigm

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Embodiment Construction

[0042] The specific implementation steps of "a KASP molecular marker method for identifying rice blast resistance gene Pigm" are as follows:

[0043] (1) Test materials (the following materials are publicly known and public, and the mid-term bank of agricultural germplasm resources in Jiangsu Province can be provided externally)

[0044] Materials containing Pigm homozygous genotype: Gumei No. 4, a Sichuan indica landrace.

[0045] Pigm-resistant homozygous genotype materials: 4 near-isogenic lines IRBL9-W(Pi9), IRBLz5-CA-1(Pi2), IRBLz-Fu(Piz), IRBLz-Fu(Piz), IRBLzt-T (Piz-t) (cited by the International Rice Research Institute, IRRI), indica rice restorer material Zhenhui 084 (Zhenjiang Institute of Agricultural Sciences in Jiangsu Hilly Region), 93-11 (Institute of Agricultural Sciences in Lixiahe Region, Jiangsu), Zhonghui 8006 (China Rice Research Institute-Zhejiang), Chenghui 727 (Sichuan Academy of Agricultural Sciences Crop Research Institute), Yihui 1577 (Sichuan Yibin...

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Abstract

The invention relates to a KASP molecular marker method for identifying rice blast resistance gene Pigm, which belongs to the field of biotechnology engineering. According to the base variation of G-C at 515bp upstream of the start codon of Pigm-R2 gene at Pigm site between Gumei 4 and the sequenced indica and japonica varieties, KASP molecular marker primers were designed to fluoresce the DNA of different rice materials. Quantitative PCR was used to amplify and determine the Pigm genotype. The method of the invention can not only quickly and accurately identify whether the rice germplasm resource or breeding population contains Pigm gene, but also can realize high-throughput detection of more sample materials at the same time. Compared with conventional molecular marker technology, it has the advantages of faster and more efficient. Therefore, this molecular marker method can further improve the selection efficiency of Pigm-containing disease-resistant homozygous genotypes in breeding, and accelerate the breeding process of rice blast-resistant rice varieties.

Description

1. Technical field [0001] The invention relates to a KASP molecular marker method for identifying rice blast resistance gene Pigm, which belongs to the field of biotechnology engineering and is dedicated to identification, screening and variety breeding of rice blast resistance germplasm resources containing Pigm gene. 2. Background technology [0002] Rice blast is a fungal disease caused by Ascomycetes (Magnaporthe oryzae (Hebert) Barr., amorph Pyriculariaoryae Cav.), and the global annual yield loss caused by rice blast accounts for 10-30% of the total rice yield (Wilson R A et al., Under pressure: investigating the biology of plantinfection by Magnaporthe oryzae, Nature Reviews Microbiology, 2009, 7(3):185-195). In my country, rice blast occurs to varying degrees every year, with an annual occurrence area of ​​about 4.0×10 6 ~6.0×10 6 hm 2 , loss of paddy 6.0×10 5 ~1.45×10 6 t, has become an important factor restricting rice production, and has also caused serious h...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2531/113C12Q2563/107C12Q2537/161C12Q2535/137
Inventor 陈涛张亚东梁文化张善磊孙旭超姚姝赵庆勇周丽慧赵春芳朱镇赵凌王才林
Owner JIANGSU ACAD OF AGRI SCI
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