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Method for producing pre-basic seeds of potatoes

An original seed, potato technology, applied in the field of agricultural cultivation, can solve the problems of serious diseases, limited development of the potato industry, and degradation of potato varieties.

Inactive Publication Date: 2012-07-11
SICHUAN AAS HORTICULTURE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the degradation of potato varieties and serious diseases, the development of potato industry is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The present embodiment is the laboratory production of potato test tube seedlings:

[0052] (1) Making a polypropylene film bag: Cut the polypropylene film into a rectangle of 35cm×10cm, fold two 2.5cm wide from 15cm, and glue the two sides together with a plastic sealing machine to make a height of 15cm and a width of 10cm of open pockets.

[0053] (2) Preparation and sub-packaging of culture medium:

[0054] The medium was prepared as follows:

[0055] ①Accurately weigh KH 2 PO 4 170mg, NH 4 NO 3 1650mg, KNO 3 1900mg, CaCl 2 ·2H 2 O 440mg and MgSO 4 ·7H 2 O 370mg, respectively placed in a 200ml beaker, add 100ml of purified water to dissolve respectively, mix the solution in a 2000ml beaker according to the order of the listed drugs, pay attention to mixing slowly, keep stirring, and stand by;

[0056] ②Accurately weigh MnSO 4 ·4H 2 O 22.3 mg, ZnSO 4 ·7H 2 O 8.6 mg, H 3 BO 3 6.2 mg,...

Embodiment 2

[0063] The present embodiment is the rapid propagation of sugar-free culture in the test-tube seedling greenhouse:

[0064] (1) Soak the test tube seedlings in the plant growth regulator solution for 20 minutes, take out, the plant growth regulator solution is 1 liter of water containing NAA 25mg, IBA 25mg and IAA 40mg at the same time. The test-tube seedlings at this time are the cutting seedlings of 1-2 stem segments with axillary buds, prepare a 50-hole plug tray with a cultivation substrate, cut the cutting seedlings into the plug holes of the plug tray, and water to water after the cutting is completed. Flow out from the bottom hole of the plug tray; the formula of the cultivation medium is sand: vermiculite: perlite = 2:2:1.

[0065] (2) Put the completed plugs into the water tank for liquid culture. There is nutrient solution I in the water tank, keep the depth of the nutrient solution at about 0.3cm, and cover the surface of the plugs with plastic film for cultiv...

Embodiment 3

[0075] The present embodiment is a matrix+liquid culture to produce the original stock:

[0076] (1) When the cuttings grow in the 4th week, replace the nutrient solution in the water tank with nutrient solution II, keep the depth at 1-2cm, and place a plastic tube with a diameter of about 1cm under the plug tray so that the bottom of the plug tray does not touch. Bottom of the sink, this process lasts 2 weeks;

[0077] Among them, the formula and preparation method of each liter of nutrient solution II are as follows:

[0078] ①Accurately weigh MnSO 4 ·4H 2 O 4.46 mg, ZnSO 4 ·7H 2 O 1.72 mg, H 3 BO 3 1.24 mg, KI 0.17 mg, Na 2 MoO 4 ·2H 2 O 0.05 mg, CoCl 2 ·6H 2 O 0.005 mg, CuSO 4 ·5H 2 O 0.005 mg, FeSO 4 ·7H 2 O 5.56 mg, Na 2 - EDTA 7.46 mg in a 200ml beaker, after adding water to dissolve, slowly pour it into a 2000ml beaker, stir and mix well, set aside;

[0079] ②Accurately weigh N:P 2 ...

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Abstract

The invention discloses a method for producing pre-basic seeds of potatoes. The method comprises the steps as follows: laboratory production of potato tube seedlings, sugar-free culture and fast propagation in a tube seedling greenhouse, and matrix and liquid culture for producing pre-basic seeds of potatoes. According to the invention, a polypropylene film bag takes the place of a traditional glass container, so that the light transmission and the air permeability are good, the cost is low, the weight is light, and the carrying is simpler; the mode of matrix and liquid culture achieves the purpose of propagating pre-basic seeds in a layered manner, so that not only can vain growth of the overground parts be controlled effectively, and growth and development of creeping stems of plants are ensured, but also forming and expanding of tubers at the top ends can be promoted effectively, and the operation is simple and easy; the pre-basic seeds can be grown in a plurality of seasons in a year, the quantity of potatoes borne in a unit area and the yield are improved greatly, the cost is reduced considerably, the propagation efficiency is improved, and industrial and mass production of virus-free miniature seed potato of potatoes can be realized.

Description

technical field [0001] The invention relates to a method for producing original potato seeds, belonging to the field of agricultural cultivation. Background technique [0002] Potato is not only one of the main food crops in my country, but also an important feed and industrial raw material crop, with high economic benefits. However, due to the degradation of potato varieties and serious diseases, the development of potato industry is limited. In recent years, by cultivating virus-free seed potatoes and establishing a breeding system for improved varieties, a key problem restricting the development of the potato industry has been effectively solved. Virus-free seed potatoes are cultivated by peeling off the 0.3mm shoot tip of potato plants to form regenerated plants. After virus serological detection, the non-toxic plants are subjected to tissue culture and rapid propagation to produce test-tube seedlings. Under the conditions of isolated production in greenhouses and net ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 何俊蓉黄刚蒋彧唐丽卓碧萍沈学善王海娥李世元
Owner SICHUAN AAS HORTICULTURE RES INST
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