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Molecular markers of paddy endosperm low amylose content gene Wx-mq

A low-amylose and molecular marker technology, applied in the field of molecular genetics, can solve the problems of reduced amylose synthesis efficiency and endosperm amylose content, and achieve the effects of improving breeding efficiency and reducing breeding costs.

Active Publication Date: 2010-08-04
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Application Information

AI Technical Summary

Problems solved by technology

The function of the semi-waxy gene Wx-mq has been preliminarily clarified. Because the Waxy gene has a single-base mutation in exon 4 and exon 5, resulting in two amino acid mutations, it may lead to amylose synthesis Reduced efficiency, resulting in lower endosperm amylose content

Method used

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  • Molecular markers of paddy endosperm low amylose content gene Wx-mq
  • Molecular markers of paddy endosperm low amylose content gene Wx-mq
  • Molecular markers of paddy endosperm low amylose content gene Wx-mq

Examples

Experimental program
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Embodiment Construction

[0021] The methods used in the following examples are conventional methods unless otherwise specified.

[0022] (1) Obtaining molecular markers to identify Wx-mq genotypes with low amylose content in endosperm

[0023] 1 test material

[0024] They are rice varieties Nanjing 41, Nanjing 44, Suhuxiangjing, Nanjing 43, Zhendao 88, Xudao 3, low amylose content varieties Nanjing 46 and Guandong 194.

[0025] 2 DNA extraction

[0026] Take about 1 gram of rice leaves at the tillering stage or booting stage, and use the SDS method to extract DNA.

[0027] DNA extraction was slightly modified with reference to the method of Dellapporta et al. (1983), and the specific steps were as follows:

[0028] ①Take 200-300 mg of fresh rice leaves (leaves stored at -20°C), grind them into powder with liquid nitrogen in a pre-cooled mortar at -20°C.

[0029] ② Transfer to a 1.5ml centrifuge tube.

[0030] ③Add 600μl SDS extract and shake well, incubate at 65°C for 30min, shake 3-4 times.

...

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Abstract

The invention relates to molecular markers of paddy endosperm low amylose content gene Wx-mq, which belong to the field of molecular genetics. Two pairs of PRC molecular marker primers for specifically amplifying paddy endosperm low amylose content control gene Wx-mq and an allelic gene Wx-b thereof are used, wherein a forward general primer 1Wx-b-F of the Wx-b and the Wx-mq, a reverse primer 11Wx-b-R of the Wx-b and a reverse primer 11Wx-mq-R of the Wx-mq perform common amplification on the DNA of japonica rice varieties or materials, and can distinguish genotypes of the Wx-mq gene and the allelic gene thereof accurately. Developed peculiar PCR markers of the allelic gene are utilized to perform the paddy endosperm low amylose seed breeding and the improvement of rice quality.

Description

technical field [0001] The invention provides a molecular marker of rice endosperm low amylose content gene Wx-mq, belongs to the field of molecular genetics, and is specially used for genotype identification of rice endosperm low amylose content control gene Wx-mq. Background technique [0002] As one of the important goals of rice breeding, rice quality has attracted more and more attention from breeders and consumers. Studies have shown that the relative content of amylose in starch is one of the important factors that determine the texture and taste of rice. The amylose content is low, the rice is sticky and soft, the appearance is oily and shiny, and it does not regenerate when cold, and the palatability is good; on the contrary, the rice is hard, less viscous, fluffy and dry, and has poor gloss. Therefore, the content of amylose has become the main indicator for improving the eating quality of rice. Rice with low amylose content is an intermediate type between ordina...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 杨杰仲维功曹卿王军陈涛范方军王才林
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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