Method for rapidly obtaining a large number of wheat double-haploid homozygous populations

A double haploid and haploid technology, applied in the field of wheat breeding, can solve problems such as inability to adapt and affect the breeding process, and achieve the effects of improving survival rate, breeding efficiency, and damage resistance

Active Publication Date: 2015-07-01
ZHOUKOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, immature embryo culture, chromosome doubling, multiplication, etc., are currently commonly used methods to produce haploid seedlings through immature embryo culture, transplant them to low-temperature greenhouses, undergo summer cultivation, autumn sowing, and wait for the next year to double into single seedlings. ploidy, and then after one year of multiplication, it will take at least two years to obtain a stable new strain with sufficient seed quantity and enter the new strain identification link, which affects the breeding process to a certain extent and cannot adapt to today's rapid development. Requirements for Breeding New Wheat Varieties with High Yield, High Quality and Multi-resistance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 ( 2013-2014 test)

[0033] A method for rapidly obtaining a large amount of wheat double haploid homozygous populations, comprising the following steps:

[0034] (1) Planting: In the Huanghuai area, wheat hybrid seeds were sown in three phases in autumn, with the sowing dates being October 10, October 25, and November 10; sweet corn seeds were planted on December 25, Sow in the greenhouse in stages on January 5, January 15, January 25, February 5, and February 15 of the following year, so that the flowering period of wheat and sweet corn can meet;

[0035] (2) Haploid induction: Wheat planted in stages enters the earing stage between April 13 and 18 of the following year, and sweet corn planted in stages also enters the powdering stage at this time, and artificial emasculation is performed 2 days after wheat earing. Bagging and isolation, 3 days after emasculation, use fresh corn pollen to pollinate the wheat flower by flower (using saturated rolling method...

Embodiment 2

[0042] Example 2 (Trial 1 from 2012 to 2013)

[0043] A method for rapidly obtaining a large amount of wheat double haploid homozygous populations, comprising the following steps:

[0044] (1) Planting: In the Huanghuai region, wheat hybrid seeds were sown in three phases in autumn, with the sowing dates being October 13, October 28, and November 13. The sweet corn seeds are sown in the greenhouse in stages on December 28, January 8, January 18, January 28, February 8, and February 18, so that the two flowering periods meet;

[0045] (2) Haploid induction: Wheat planted in stages enters the earing period from April 16 to 28 of the following year, and sweet corn planted in stages gradually enters the powdering stage at this time; 3 days after wheat earing, manually detassell, bagging Isolation, 3 days after emasculation, use fresh corn pollen to pollinate the wheat flower by flower, or use saturated rolling pollination to increase the pollination rate, and still bag and isol...

Embodiment 3

[0052] Example 3 (Trial 2 from 2012 to 2013)

[0053] A method for rapidly obtaining a large amount of wheat double haploid homozygous populations, comprising the following steps:

[0054] (1) Planting: In the Huanghuai region, wheat hybrid seeds were sown in three phases in autumn, with the sowing dates being October 13, October 28, and November 13. The sweet corn seeds are sown in the greenhouse in stages on December 28, January 8, January 18, January 28, February 8, and February 18, so that the two flowering periods meet;

[0055] (2) Haploid induction: Wheat planted in stages enters the earing stage from April 1 to 28 of the following year, and sweet corn planted in stages gradually enters the powdering stage at this time; artificial detasseling is performed 2 days after wheat earing, and bagged Isolation, 3 days after emasculation, use fresh corn pollen to pollinate the wheat flower by flower, or use saturated rolling pollination to increase the pollination rate, and s...

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PUM

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Abstract

The invention discloses a method for rapidly obtaining a large number of wheat double-haploid homozygous populations. According to the method, based on a wheat and corn double-haploid breeding technology, through the key links of wheat and corn sowing at different stages, haploid induction, transplantation and vernalization, reduplication and transplantation and winter sowing treatment, after wheat young embryos differentiate into haploid seedlings, transplantation, vernalization and haploid plant reduplication are completed in the same year without over-summer treatment, wheat double-haploid seeds can be harvested in the same year, the requirement for sowing once more can be met after the harvest on the perspective of time, a large number of stable new strains of the wheat homozygous populations can be obtained within one year, the requirement for identifying the new strains at the next stage is met, the natural environment is utilized to the maximum degree, and the time of wheat double-haploid breeding is shortened to one year from two to three years. In this way, the wheat breeding speed is increased, the breeding efficiency is improved, the breeding cycle is shortened, and the breeding cost is lowered.

Description

Technical field [0001] The present invention belongs to the field of wheat breeding technology, which involves a method that quickly obtains a large number of wheat double polymers. Background technique [0002] The conventional wheat breeding technology needs to obtain a solid new product system through the selection, sexual hybridization, separation of offspring, artificial selection, gene pure convergence, and new product identification.Time, the selection process of a new product takes about 10 years, which is time -consuming and laborious. [0003] Wheat double -doubling sports breeding time is gradually adopted by breeding experts and commercial breeding enterprises because of its acceleration of wheat breeding process and shortening the new varieties of wheat varieties.Wheat double -doubling sports species is a wheat monotherapy body disappear through wheat × corn hybridization and hybridization. The wheat monotherapy and their chromosomes are doubled after forming a pure ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01H1/08A01H4/00
Inventor 高艳唐建卫殷贵鸿韩玉林黄峰王丽娜李顺成吕永军李楠楠张倩于海飞杨光宇李新平
Owner ZHOUKOU ACAD OF AGRI SCI
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