A method to quickly obtain a large number of double haploid homozygous populations in wheat

Abstract

The invention discloses a method for rapidly obtaining a large number of wheat double-haploid homozygous populations. According to the method, based on a wheat and corn double-haploid breeding technology, through the key links of wheat and corn sowing at different stages, haploid induction, transplantation and vernalization, reduplication and transplantation and winter sowing treatment, after wheat young embryos differentiate into haploid seedlings, transplantation, vernalization and haploid plant reduplication are completed in the same year without over-summer treatment, wheat double-haploid seeds can be harvested in the same year, the requirement for sowing once more can be met after the harvest on the perspective of time, a large number of stable new strains of the wheat homozygous populations can be obtained within one year, the requirement for identifying the new strains at the next stage is met, the natural environment is utilized to the maximum degree, and the time of wheat double-haploid breeding is shortened to one year from two to three years. In this way, the wheat breeding speed is increased, the breeding efficiency is improved, the breeding cycle is shortened, and the breeding cost is lowered.

Description

technical field [0001] The invention belongs to the technical field of wheat breeding, and in particular relates to a method for rapidly obtaining a large number of homozygous double haploid populations of wheat. Background technique [0002] Conventional wheat breeding technology needs to obtain homozygous and consistent stable new strains through parent selection, sexual hybridization, offspring segregation, artificial selection, gene homozygosity, and new strain identification. It takes 6 to 8 years for the offspring to start to segregate to gene homozygosity Time, the breeding process of a new strain takes about 10 years, which is time-consuming and labor-intensive. [0003] Wheat double haploid breeding is gradually being adopted by breeding experts and commercial breeding enterprises because it can speed up the wheat breeding process and shorten the breeding time of new wheat varieties. Wheat double haploid breeding is to obtain wheat haploid through wheat × corn hybr...

AI Technical Summary

Problems solved by technology

Among them, immature embryo culture, chromosome doubling, multiplication, etc., are currently commonly used methods to produce haploid seedlings through immature embryo culture, transplant them to low-temperature greenhouses, undergo summer cultivation, autumn sowing, and wait for the next year to double into single seedlings. ploidy, and then after one year of multiplication, it will take at least two years to obtain a stable new strain with sufficient seed quantity and enter the new strain identification link, which affects the breeding process to a certain extent and cannot adapt to today's rapid development. Requirements for Breeding New Wheat Varieties with High Yield, High Quality and Multi-resistance