Method for intergeneric hybridization transfer of clubroot-resistant genes of radishes and brassica napus

Abstract

The invention discloses a method for intergeneric hybridization transfer of clubroot-resistant genes of radishes and brassica napus. The method specifically comprises the following steps of identifying and screening disease-resistant radishes; carrying out distant hybridization of the brassica napus and the disease-resistant radishes, specifically, obtaining intergeneric hybrid seeds by using a method of combining sexual hybridization with ovary culture so as to obtain clubroot-resistant bridge materials; and carrying out backcross screening of the hybrid seeds and the brassica napus, specifically, doubling the F1 generation true hybrid seeds to obtain S0, backcrossing the S0 with the brassica napus, carrying out resistance identification on BC<1> generation, and screening disease-resistant plants to serve as an intermediate material for continuously cultivating disease-resistant varieties of the brassica napus. According to the method for intergeneric hybridization transfer of the clubroot-resistant genes of the radishes and the brassica napus, through disease resistance identification, sexual hybridization and ovary culture methods, in combination with morphology, cytology and molecular detection technologies, the clubroot-resistant genes in the radishes are introduced into the brassica napus, breeders carry out selection and breeding many years to obtain high-resistance high-quality brassica napus varieties, the high-resistance high-quality brassica napus varieties are used for planting by farmers in regions with high clubroot disease incidence of the brassica napus, andthe planting income is increased.

Description

technical field [0001] The invention relates to the technical field of disease-resistant plants, in particular to a method for hybridizing and transferring clubroot disease-resistant genes between radish and Brassica napus. Background technique [0002] Clubroot (Clubroot) is commonly known as root nodule, that is, the roots of plants are enlarged and deformed, similar to tumors. The disease mainly occurs on cruciferous crops and is caused by the protist P. brassicae Woron infecting its roots. It is a worldwide soil-borne disease with a long history and is difficult to control. Plasmodium brassica can infect almost all cruciferous crops, and cultivated crops are more seriously affected, including all varieties of cabbage, rapeseed, and turnips. After the plant is infected, there will be no symptoms at the initial stage. After a period of time, the root parenchyma cells begin to divide abnormally, and the cells become enlarged, forming a so-called tumor. In severe cases, tum...

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Problems solved by technology

But field resistance is rapidly broken down in the field, probably due to strong selection pressure

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