The invention discloses a method for culturing wheat haploid embryo test-tube seedlings. The method comprises the following steps that when each of the wheat haploid embryo test-tube seedlings grows to have 2-3 leaves, the seedlings are transferred to a strong seedling culture box for strong seedling culture; when 50% of strong seedlings in the strong seedling culture box reach a three-leaf stage, strong seedling culture is performed; after the strong seedling culture is performed for 2-3 weeks, nutritional supplementing liquid is added; when the test-tube seedlings are cultured to produce 2-3 tillers, the seedlings are transferred to a culture box for over-summering, over-summering culture is performed, and nutritional supplementing liquid is added; from the last ten days of October to the first ten days of November, the culture conditions of the strong seedling culture box are recovered, the seedlings are transplanted and hardened when the field temperature is reduced to 15 DEG C or below, and the seedlings are transplanted in open ground in 2-3 days after seedling hardening. The nutritional supplementing liquid is added at different stages, the wheat haploid embryo test-tube seedlings can grow into one-step strong seedlings through one step, the over-summering decline and fall of the test-tube seedlings are simply and effectively relieved, the survival rate of the test-tube seedlings and survival rate of bottling out and transplantation are improved, and the doubling efficiency of the haploid seedlings is improved indirectly.