Method for Inducing Haploid in Maize

A technique of haploid and haploid induction, which is applied in chemical instruments and methods, botanical equipment and methods, and the use of vectors to introduce foreign genetic materials, etc., can solve the problems of inconvenient operation, high production cost, and low commercial application value. advanced questions
CN104335889BInactive Publication Date: 2016-03-02CHINA AGRI UNIV +1
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2016-03-02
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a method for inducing corn haploids. The method comprises the following steps: pollinating a female parent by a male parent corn haploid induction line with a higher corn haploid inductivity than a corn haploid induction line CAU5, and harvesting hybridized F1 generation grains to obtain corn haploids. A method for realizing the corn haploid induction line comprises the following steps: 1, introducing coding gene of a specific centromere histone mutant into a hybrid first generation plant of a corn inbred line HiIIA and a corn inbred line HiIIB to obtain a transgenic corn plant; and 2, hybridizing the specific histone mutant as a female parent with the CAU5 to obtain an F1 generation, hybridizing the F1 generation as a female parent with the CAU5 to obtain a BC1F1 generation, hybridizing the BC1F1 generation as a female parent with the CAU5 to obtain a BC2F1 generation, and continuously selfing the BC2F1 generation at least five generations to obtain the corn haploid induction line.
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Description

technical field

[0001] The present invention relates to a method for inducing haploid in maize. Background technique

[0002] Haploid refers to an individual with the number of gamete chromosomes. Since the number of haploid chromosomes is only the number of gametophyte chromosomes, once doubled, pure lines can be obtained, and pure line selection is a very critical link in the utilization of heterosis, so the utilization value of haploid is undoubtedly huge.

[0003] Haploid breeding refers to the way of obtaining haploids by various methods, obtaining homozygous diploids through chromosome doubling, and selecting at the haploid and diploid levels in order to breed excellent pure-line varieties. In 1964, haploid plants were successfully obtained by culturing datura anthers (Guha and Maheshwari, 1964), followed by an upsurge in haploid research, which developed rapidly. The advantages of haploid breeding are as follows: 1) Shorten the breeding process. It usually takes 6 t...

Claims

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