Method for creating corn autotetraploid

Abstract

The invention relates to a method for creating corn autotetraploid. The method comprises the following steps: treating corn filaments by adopting a conventional chromosome doubling agent and a conventional treatment method; doubling somatic chromosomes in induced organs without the need of pollination, so as to directly obtain autotetraploid seeds; then carrying out microscope examination on chromosome ploidy and screening the autotetraploid; carrying out fruitfulness breeding when the fructification percentage is relatively low; preparing autotetraploid hybrid seeds by utilizing an autotetraploid selfing line after the fructification percentage reaches the requirements. The method provided by the invention has the beneficial effects that the method is simple and feasible; seed germination, germ low-temperature treatment and seedling transplanting processes of an existing treatment method are omitted; the doubling efficiency is improved, and the problem that a chromosome ploidy chimerarepels tetraploid cells due to competitive advantages of diploid cells in a somatic cytotropism development process is avoided, so that the doubling efficiency is improved; the yield and quality areimproved and the purity is easy to ensure.

Description

Technical field

[0001] The invention belongs to the technical field of corn breeding, in particular to the homologous quadruple species of sweet waxy corn and silage corn. Background technique

[0002] The corn varieties produced are diploid plants. In order to meet new demands, people have adopted the method of artificially doubling chromosomes to create autotetraploid maize. Compared with diploid maize, autotetraploid maize has the characteristics of tall plants, luxuriant stems and leaves, large grains and high yield. Therefore, the homologous quadruple species can be used as a breeding way to improve the biological yield of silage corn and to improve the quality and yield of sweet corn, waxy corn and ordinary corn.

[0003] In maize breeding research at home and abroad, autotetraploid is used as a way to innovate germplasm and breed new tetraploid maize varieties to increase grain and vegetative yield, increase grain weight and nutritional value. In the prior art, the method...