Breeding method and application of corn haploid induction line

A haploid inducible line and the technology of the inducible line are applied in the field of crops, which can solve the problems of unfavorable haploid grain selection, unsuitable haploid young embryos, and light color of endosperm, and achieve good pollen vigor and young embryos. The effect of early color and good comprehensive traits

Inactive Publication Date: 2021-10-01
北大荒垦丰种业股份有限公司
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Problems solved by technology

[0008] Although the currently used haploid induction line KY8556 has a good induction rate and a large amount of loose powder, the color development of immature embryos is relatively late, and it is difficult for immature embryos with a length of less than 3 mm to develop color, and it takes 24 hours of in vitro light to develop color. , not suitable for early identification and selection of haploid immature embryos; the endosperm of mature grains is lighter in color and smaller in area, which is also not conducive to the selection of haploid grains

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  • Breeding method and application of corn haploid induction line
  • Breeding method and application of corn haploid induction line
  • Breeding method and application of corn haploid induction line

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Embodiment 1

[0074] This example mainly relates to the breeding of maize haploid induction lines and the method of chromosome doubling in haploid tissue culture. The maize haploid induction line KY8556 and a resource material S8360 whose early immature embryos were purple were crossed as parents, and the F1 offspring were backcrossed with KY8556, and the immature embryos were stripped and lighted 13 days after pollination, and the immature embryos with early and obvious coloration were selected for tissue culture Seedlings were grown and cultivated in the greenhouse. Molecular marker analysis was carried out during the period, and 5 individual plants carrying the ZmPLA gene with a homozygous 4bp insertion mutation and a high recovery rate of genetic background were screened. Backcross, repeat above-mentioned flow process, finish 5-6 times of backcross altogether, self-crossing is firm and harvests fringe ( figure 1 ). Sow seeds in the field, select lines with uniform progeny and good comp...

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Abstract

The invention belongs to the field of crops, particularly relates to a breeding method and application of a corn haploid induction line, and mainly discloses the breeding method for breeding the corn haploid induction line. The breeding method for breeding the corn haploid induction line comprises the following steps of hybridizing a haploid induction line in a corn resource library with a material with a purple early immature embryo, performing multi-generation backcross, and selfing to obtain the corn haploid induction line. The method can be used for rapidly breeding the corn haploid induction line which is good in comprehensive character, high in induction rate and suitable for haploid early-stage immature embryo selection.

Description

technical field [0001] The invention belongs to the field of crops, and in particular relates to a method for breeding a maize haploid induction line and its application. Background technique [0002] The creation and selection of inbred lines is the core of maize hybrid breeding. Conventional hybridization and multiple rounds of backcrossing methods require six generations of backcrossing and two generations of selfing for a total of eight generations to obtain a stable inbred line with a homozygous rate higher than 99%. , It takes several years, the cost is high, and the cycle is long. The maize haploid doubling technology developed in recent years, by crossing the pollen of the haploid inducing line with high-quality genetic resource materials, some of the offspring produced are induced to become haploid, and then these haploid embryos germinate and become seedlings in specific conditions. stage with colchicine and other cell division inhibitors to induce chromosome doub...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H4/00
CPCA01H1/021A01H1/04A01H4/008
Inventor 李忠森邓艳雪刘丹张亚田赵英男孙树民
Owner 北大荒垦丰种业股份有限公司
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