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Method for doubling management of corn haploid

A haploid and chromosome doubling technology, which is applied in the fields of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of serious damage to plants, high toxicity of colchicine, unsatisfactory doubling efficiency of seed soaking method, etc. The effect of doubling efficiency and improving breeding efficiency

Inactive Publication Date: 2011-09-14
CHINA AGRI UNIV
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Problems solved by technology

But utilize injection method if processing period and the position of injection are inaccurate, will greatly influence the effect of this method; Utilize soaking root method to damage more serious to plant, survival rate is lower (wenke, Li Liang, Liu Yuqiang, Chen Shaojiang, 2006. Preliminary report on efficient biological induction of maize haploid and its doubling method, Journal of China Agricultural University, 11(5):17-20); Gayen et al. took colchicine to soak haploid seeds, but only 18% of the seeds doubled Success (Gayen P., Maden J.K., Kumar R. and Sarkar K.R., 1994. Chromosome doubling in haploids through colchicines. Maize Genet Coop News Lett. 68: 64-65), the overall doubling efficiency of soaking seeds is not ideal
At present, on the whole, the doubling effect of herbicides is far inferior to colchicine, so colchicine is the most commonly used high-efficiency doubling reagent for haploid doubling, but colchicine is very toxic and can easily cause serious damage to seedlings. Damage, if not managed well will affect the doubling efficiency of haploids

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  • Method for doubling management of corn haploid
  • Method for doubling management of corn haploid
  • Method for doubling management of corn haploid

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Embodiment 1

[0023] Embodiment 1, carry out the doubling of corn haploid with colchicine mixed solution of different concentrations

[0024] 1. Screening of maize haploid seeds

[0025] The excellent hybrid Zhengdan 958 (Beijing Denong Seed Industry Co., Ltd.) and the stable and high-quality hybrid Nongda 108 (Beijing Golden Nonghua Seed Industry Technology Co., Ltd.) were used as female parents, and the parthenogenetic haploid induction line Nongdagao was used. No. 1 (the public can obtain from China Agricultural University, and the non-patent literature that has recorded this material is: Liu Zhizeng, Song Tongming, 2000, Breeding and identification of maize high-frequency parthenogenic haploid induction line, Acta Crops, 26 (5 ):570-574) were induced as male parents. The seeds with purple marks in the aleurone layer and no purple marks in the embryo were selected as haploid seeds. A total of 671 haploid seeds of Zhengdan 958 and 466 haploid seeds of Nongda 108 were selected.

[0026] ...

Embodiment 2

[0090] Embodiment 2, the corn haploid of different basic materials is doubled with colchicine mixture

[0091] 1. Screening of maize haploid seeds

[0092] The maize hybrids Nongda 108 (Beijing Golden Nonghua Seed Industry Technology Co., Ltd.) and Zhengdan 958 (Beijing Denong Seed Industry Co., Ltd.) were used as female parents, and the parthenogenetic haploid induction line Nongda Gaoyu No. The father is induced. Haploid seeds were selected with purple marks in the aleurone layer and no purple marks in the embryo.

[0093] 2. Double the corn haploids with different basic materials with colchicine mixture

[0094] Method I

[0095] 1. Pregermination of corn haploid seeds

[0096] Wash the haploid seeds screened in step 1 with water, and place them in a tray covered with sterilized towels, leaving a certain gap between the seeds. Then put it into an incubator and cultivate it in the dark to obtain haploid maize sprouts.

[0097] 2. Preparation of colchicine mixture

[0...

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Abstract

The invention discloses a method for doubling management of corn haploid. The method for doubling management of corn haploid comprises the following steps: (1) cutting the tip of coleoptile of the corn haploid bud, but not damaging the main leaf and the growing point to obtain the corn haploid bud with coleoptile tip cut; and (2) soaking the corn haploid bud with coleoptile tip cut with a mixed solution of colchicine, dimethyl sulfoxide and water to obtain a chromosome doubled corn bud. The method for doubling management of the corn haploid can remarkably improve the doubling efficiency of the corn haploid, and the tassel pollination rate is over 30 percent which is much higher than the contrast tassel pollination rate (lower than 5 percent). The method solves the vital technical problem of low doubling efficiency in the haploid breeding process, so that the haploid breeding efficiency is greatly improved, and large-scale application of the haploid breeding technology becomes possible.

Description

technical field [0001] The invention relates to a management method for doubling of corn haploids. Background technique [0002] Breeding of elite pure lines is the core link of maize breeding. Using traditional breeding methods, it generally takes 4-6 generations of selfing to make the line homozygous (Kuo C.S., Guo Z.C., Li Z., Gui Y., 1994.Anther culture and haploid breeding of maize in China.In: Bajaj(Ed.), Biotechnology in Agriculture and ForestryVol.25, Maize, Springer Verlag, Berlin, 149-161); and theoretically speaking, even if selfing n(n∝∞) generation, the genotype can not reach 100% completely homozygous state. Therefore, it usually takes decades to breed a variety, which consumes a lot of manpower, material resources and financial resources. Therefore, how to shorten the breeding process and improve the breeding efficiency is the goal that breeders have been pursuing. However, the haploid breeding technology only needs two generations to obtain a pure line wit...

Claims

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Application Information

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IPC IPC(8): A01H1/08
Inventor 陈绍江李浩川黎亮
Owner CHINA AGRI UNIV
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