A method for improving the chromosome doubling efficiency of triploid Echinacea purpurea induced by colchicine

A technology of colchicine and triploid, which is applied in the field of crop biotechnology and genetic breeding, can solve the problems of low success rate of polyploid induction, and achieve the effect of overcoming the poor doubling effect.

Inactive Publication Date: 2016-03-30
SOUTH CHINA AGRI UNIV
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  • Description
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  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the defect of low success rate of polyploid induction induced by explants of Echinacea purpurea petiole in the prior art, and to provide a method for improving the chromosome doubling efficiency of triploid Echinacea purpurea induced by colchicine

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  • A method for improving the chromosome doubling efficiency of triploid Echinacea purpurea induced by colchicine
  • A method for improving the chromosome doubling efficiency of triploid Echinacea purpurea induced by colchicine
  • A method for improving the chromosome doubling efficiency of triploid Echinacea purpurea induced by colchicine

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Embodiment 1

[0025] The influence of embodiment 1 colchicine on the survival state of different types of explants

[0026] The diploid seeds of Echinacea purpurea used in the present invention are purchased from the Plantation Products horticultural company of Norton, Massachusetts; tetraploid is carried out colchicine treatment to diploid Echinacea purpurea by applicant's existing method and its chromosome doubling is obtained (Nilanthi, 2009 ); triploids are obtained by crossing diploids with tetraploids and cultivating seeds.

[0027] Take the leaves, petioles and root explants of the obtained triploid Echinacea purpurea respectively and treat them in the medium containing 120mg / L colchicine for 18 days, then transfer to the medium containing BA0.4mg / L, NAA0.01mg / L, sucrose 30g / L, cultured on the MS regeneration medium of agar 4.5g / L, and the explant survival rate was counted after 22 days. As can be seen from Table 1, different types of triploid explants have significant difference...

Embodiment 2

[0029] The effect of embodiment 2 root explant selection position on induction of adventitious buds after colchicine treatment

[0030] For the same triploid line, after 30 days of rooting, select roots that are close in state, and intercept different positions (near the root tip, middle, and far from the root tip). .4mg / L, NAA0.01mg / L, sucrose 30g / L, agar 4.5g / L MS medium, cultivated under the conditions of 23~27℃, cool white light intensity 600~1300lx, light cycle 12h / d for 25 days, and then induce regeneration of adventitious buds on MS medium containing 0.5mg / LBA, and study the rate of adventitious bud induction. According to Table 2, as long as the roots are rooted at the same time, and the selected roots are close to each other, no matter whether the root section close to the root tip is used as the explant or the root section far away from the root tip is used as the explant, the After doubling the treatment with narcissine, there was no significant difference in the...

Embodiment 3

[0032] After embodiment 3 colchicine treatment, the influence of different concentrations of BA on the induction of adventitious buds

[0033] The triploid root explants treated with colchicine at a concentration of 120 mg / L for 25 days were inoculated on MS medium containing different concentrations of BA (0.3, 0.7, 1.4 mg / L). 1300lx, 12-h photoperiod environment, after 45 days of culture, observe and count the number of regenerated shoots on the unit explant. According to Table 3, the explants treated with colchicine had the highest ratio of regenerated buds at 0.7 mg / LBA, and each explant produced 0.38 adventitious buds on average. When the concentration of BA was 0.3mg / L, no adventitious shoots were regenerated.

[0034]

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Abstract

The invention belongs to the technical fields of crop biotechnology and genetic breeding, and specifically discloses a method for improving the chromosome doubling efficiency of triploid Echinacea purpurea induced by colchicine. That is, the root explants of triploid Echinacea purpurea were transferred to a medium containing 120 mg / L colchicine and cultured for 18-25 days. The ratio of successfully obtaining hexaploid Echinacea purpurea from the roots of the present invention as explants reaches 64.71%, which is at least 41.21% higher than the doubling success rate of Echinacea purpurea obtained in the prior art. The present invention effectively overcomes the three The chromosome doubling effect of ploidy Echinacea purpurea is poor, and it is difficult to obtain hexaploid strains.

Description

technical field [0001] The invention relates to the technical fields of crop biotechnology and genetic breeding, and more specifically relates to a method for improving the chromosome doubling efficiency of triploid Echinacea purpurea induced by colchicine. Background technique [0002] Echinacea ( Echinacea purpurea ) is a perennial herb of Compositae, a wild flower native to America, which has been widely used in nutritional supplements and health food because of its good immune regulation effect. Since the active ingredients of Echinacea purpurea mainly exist in the roots, in order to increase the yield of roots, further improve the quality, increase the content of active ingredients, and enhance its disease resistance, the research on ploidy breeding around it has developed rapidly. Explants successfully cultivated tetraploid, octaploid research, but the induction success rate is low. Contents of the invention [0003] The technical problem to be solved by the presen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 杨跃生陈晓鹭陈荣吴鸿李栋梁刘颖
Owner SOUTH CHINA AGRI UNIV
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