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A kind of Iris free pollen culture method

A technology of pollen culture and culture medium, applied in the field of plant tissue culture, which can solve the problems of limitations, difficulty in harvesting mature seeds, difficulty in seed collection, etc., and achieve the effects of fast speed, genetic stability and low mutation rate

Inactive Publication Date: 2016-08-24
ZHOUKOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, mature embryos are generally used in the vegetative reproduction of Ma Lin, but it is difficult to collect seeds, and it is not easy to harvest mature seeds, so it will be limited in practical application.

Method used

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  • A kind of Iris free pollen culture method

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Effect test

specific Embodiment 1

[0033] A method for cultivating free pollen of Iris japonica, comprising:

[0034] 1) Selection and disinfection of explants: get the anthers in the single-nucleus marginal stage and carry out disinfection treatment;

[0035] 2) inoculation culture: inoculate the callus induction medium, and cultivate to obtain the callus;

[0036] 3) subculture proliferation culture: the uncontaminated callus is transferred to the first subculture medium to make the buds proliferate, subcultured 5 times, transferred to the second subculture medium for subculture once, and then transferred to the first subculture medium. Continue to proliferate in the subculture medium, repeat the above steps according to the amount of seedlings needed;

[0037] 4) rooting culture: inoculate the rooting medium, and screen to obtain single plants;

[0038] 5) Seedling hardening and transplanting: first move the culture bottle to the greenhouse, harden the seedlings for 5 days under natural conditions, and whe...

specific Embodiment 2

[0047] A method for cultivating free pollen of Iris japonica, comprising:

[0048] 1) Selection and disinfection of explants: get the anthers in the single-nucleus marginal stage and carry out disinfection treatment;

[0049] 2) inoculation culture: inoculate the callus induction medium, and cultivate to obtain the callus;

[0050] 3) Subculture proliferation culture: transfer the uncontaminated callus tissue into the first subculture medium to proliferate the shoots, subculture for 5 times, transfer to a blank medium without adding any hormone for subculture once, and then transfer to Into the first subculture medium to continue to proliferate, repeat the above steps according to the amount of seedlings needed;

[0051] 4) rooting culture: inoculate the rooting medium, and screen to obtain single plants;

[0052] 5) Hardening and transplanting: first move the culture bottle to the greenhouse, and harden the seedlings for 3 days under natural conditions. When the Irriga japoni...

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Abstract

The invention discloses a free pollen cultivation method of iris ensata thunb. The free pollen cultivation method comprises the following steps: selecting and disinfecting an explant; carrying out inoculated culture; culturing to obtain a callus; carrying out subculture reproduction culture; carrying out rooting culture; and hardening seedlings and transplanting and the like. According to the free pollen cultivation method, iris ensata thunb pollen is used as the explant, iris ensata thunb can be rapidly reproduced, and the reproduction efficiency is high; in the subculture reproduction process, the unpolluted callus is transferred into a first subculture medium to reproduce buds; and after being sub-cultured for 5 times, the buds are transferred to a second subculture medium or a blank culture medium without any hormone to be sub-cultured for one time, and then are transferred into the first subculture medium to be continually reproduced, so that the variation rate in a subculture process is greatly reduced by the operation and the reproduction efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for cultivating free pollen of Iris japonica. Background technique [0002] Ma Lin, a perennial dense herb. The rhizome is thick, woody, slanting, covered with a large number of dense reddish-purple broken old leaf sheaths and hair-like fibers. Born in wastelands, roadsides, hillside grasslands, especially on overgrazing saline-alkaline pastures. Saline-alkali-resistant, trample-resistant, well-developed root system, can be used for soil and water conservation and improvement of saline-alkali soil. [0003] Ma Lin also has important medicinal, feeding and industrial values. Ma Lin has a long utilization period, high grass yield, and rich nutrients. It is a favorite food for all kinds of livestock, especially sheep. The flowers, seeds and roots of Ma Lin can be used as medicine. Dried flowers are diuretic and laxative; seeds and roots can remove dampness...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 赵锦慧胡利宗张帆郭宁何乐周琳古红梅
Owner ZHOUKOU NORMAL UNIV
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