A method for doubling corn haploid seedlings

A haploid, corn technology, applied in the directions of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of tassel abortion, shriveled anther, high toxicity, etc., and achieves good doubling effect, good application prospect, The effect of improving the loose powder rate

Active Publication Date: 2022-02-18
金苑(北京)农业技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Because the haploid has only one set of chromosomes, generally the ear is relatively normal, can be silked, and can be fruited after pollination, but most of the material tassels are aborted, have no anthers or anthers are shriveled, and cannot loose pollen normally, and chemical doubling treatment must be carried out to Meet breeding needs
At present, the doubling methods mainly include natural doubling, bud tip cutting, growth point injection, immature embryo culture, etc. The natural doubling efficiency is low, but bud tip cutting, growth point injection and immature embryo culture techniques need to be very skilled, and in the process of doubling Colchicine is used, which is highly toxic to humans, so be very careful when handling it

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, corn haploid doubling method

[0042] The corn haploid doubling method provided by the present embodiment consists of the following steps:

[0043] 1. Sowing seedlings

[0044] In the first ten days of November in winter, at the Nanbin Farm Breeding Base in Yazhou District, Sanya City, Hainan Province, 960 grains of purple top white embryo corn haploid kernels obtained by crossing Nongda Gaoyu No. 1 and Veike 702 were sown on demand using river sand as the medium Seedlings are grown in commercially available 96-hole seedling trays, river sand needs to be sieved, stones are removed, the sowing depth is 2cm, and 960 haploid seeds are sown in 10 seedling trays. Lay a micro-spray belt for every two rows of seedling trays, water it thoroughly immediately after sowing; spray water for 2 minutes every morning to keep the river sand moist, and obtain haploid seedlings;

[0045] 2. Haploid doubling

[0046] At the 3-leaf stage, in the morning, take the seedling ...

Embodiment 2

[0062] Embodiment 2, corn haploid doubling method

[0063] The corn haploid doubling method provided by the present embodiment consists of the following steps:

[0064] 1. Sowing seedlings

[0065] In late April in spring, at the Jinyuan Breeding Base in Shunyi District, Beijing, a simple plastic shed was erected with wooden stakes, and 960 haploid kernels of purple-topped white embryo corn obtained by crossing Nongda Gaoyu No. 1 with Weike 702 were sown on demand. Seedlings are raised in commercially available 96-hole seedling trays with river sand as the medium. The river sand needs to be sieved to remove stones. The sowing depth is 1.5-2 cm. Take 960 haploid seeds and sow 10 seedling trays in total. Lay a micro-spray belt for every two rows of seedling trays, water it thoroughly immediately after sowing; spray water for 2 minutes every morning to keep the river sand moist, and obtain haploid seedlings;

[0066] 2. Haploid doubling

[0067] At the 3-leaf stage, in the mor...

Embodiment 3

[0075] Embodiment 3, optimization exploration of corn haploid doubling method

[0076] 1. Exploration of podophyllotoxin solutions with different concentrations

[0077] 1. Sowing seedlings

[0078] 960 haploid grains of purple top white embryo corn obtained by crossing Nongda Gaoyu No. 1 with Veike 702 were sown and raised according to Step 1 of Example 1;

[0079] 2. Haploid doubling

[0080] Preparation of podophyllotoxin solutions with different concentrations: Dissolve 0.1g of podophyllotoxin in 100ml of 95% ethanol aqueous solution to obtain podophyllotoxin mother solution; then take 10ml of podophyllotoxin mother solution and dilute with 20L, 10L, 200ml, and 10ml of purified water to concentrations of 0.0005mg / ml, 0.001mg / ml, 0.05mg / ml, 1mg / ml.

[0081] It is basically the same as Step 2 of Example 1, except that the 0.05 mg / ml podophyllotoxin solution is replaced by podophyllotoxin solutions with different concentrations.

[0082] 3. Transplant to field: same as em...

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Abstract

The invention discloses a method for doubling corn haploid seedlings. The invention provides a method for doubling plant haploid seedlings, comprising the following steps: 1) soaking the roots of plant haploid seedlings grown to the 1-4 leaf stage in podophyllotoxin solution to obtain treated plant seedlings; 2) transplanting the treated plant seedlings to the field to realize the doubling of plant haploid seedlings. The doubling technology of corn haploid seedlings provided by the invention can significantly improve the powder loosening rate of the corn haploid, which is as high as 70%, which is far higher than that of the control (generally about 10%). The podophyllotoxin used in this method is weakly volatile, and the operator only needs to wear disposable gloves and masks to operate; at the same time, the haploid doubling is carried out by means of shed or open-field seedling cultivation and transplanting, which does not depend on laboratory operations and is suitable Large-scale batch production of double haploids.

Description

technical field [0001] The invention relates to the field of agricultural plant breeding, in particular to a method for doubling corn haploid seedlings. Background technique [0002] Corn is already the food crop with the largest planting area and the highest total yield in my country. At present, almost all the corn varieties used in production are hybrids formed by inbred lines. In long-term practice, corn breeders have such Consensus: "The difficulty lies in the selection of the system, the focus is on the combination, and the core is the problem of synergy." The traditional pedigree method of breeding inbred lines adopts the method of continuous selfing, and it takes at least 8 generations from the composition to stability, and through the method of inducing haploid re-doubling, two generations are highly homozygous and stable, which is called double single Breeding technology, because it has the advantages of stable and fast line selection, high homozygosity, accurate t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06A01G22/20
CPCA01H1/06A01G22/20
Inventor 范弘伟侯丽宏叶凯王瑞艳康广华
Owner 金苑(北京)农业技术研究院有限公司
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