Method for efficiently obtaining chimera-free tetraploid cortex magnoliae officinalis plants and cells

Abstract

The invention discloses a method for efficiently obtaining chimera-free tetraploid cortex magnoliae officinalis plants and cells. The method comprises the following steps of preparing embryogenic cellinduction materials of which the pore diameter is 200-100[mu]m, treating the embryogenic cells of 200-100[mu]m with colchicine of different concentrations, enabling the treated embryogenic cells to grow and plants to regrow, identifying tetraploid cells and plants, and domesticating and transplanting cortex magnoliae officinalis tetraploid cell differentiation embryos, somatic embryo differentiation plants and tetraploid cortex magnoliae officinalis tissue culture seedlings. According to the method for efficiently obtaining chimera-free tetraploid cortex magnoliae officinalis plants and cells, the redoubling efficiency of cortex magnoliae officinalis diploid can be notably improved, a key technology difficult problem that in the polyploid breeding process, chimeras are easy to generate, is solved, the polyploid breeding efficiency is greatly improved, and large-scale application of a polyploid breeding technique to generate the chimera-free tetraploid cortex magnoliae officinalis plants and cells is possible to realize.

Description

technical field [0001] The invention belongs to the technical field of biological breeding, and in particular relates to a method for efficiently obtaining tetraploid magnolia bark plants and cells without chimera. Background technique [0002] Magnolia officinalis Rehd. et Wils. is a plant of the genus Magnolia in the family Magnoliaceae. Magnolia officinalis bark, root bark, branch bark and flowers and fruits can be used as medicine. Magnolia officinalis bark is a traditional Chinese medicine with a history of more than 2,000 years of medicinal use in my country. The main active ingredients of magnolia bark include magnolol, honokiol, magnolol, volatile oil, β-cineole, etc., wherein the main active ingredients magnolol (C18H18O2) and honokiol (C18H18O2) have antitumor , Antibacterial, anti-ulcer, anti-depressant and anti-caries effects. Magnolia officinalis not only has important medicinal value, but also has high ornamental value. Due to its medicinal value, wild Magn...

AI Technical Summary

Problems solved by technology

[0004] Chimerism is a common problem encountered in polyploid breeding at present. The doubled tissue or cells cannot be separated from the chimera in time, and will be surrounded by vigorously growing normal diploid cells, and eventually disappear, and the plant returns to its original state. ploidy, resulting in induction failure

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