Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for breeding double haploid of corn*rice distant hybridization

A technology of distant hybridization and double haploid, which is applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., can solve problems such as low efficiency, dyeing variation, and large plant toxicity, and achieve simplified procedures, low cost, The effect of low component concentrations

Inactive Publication Date: 2019-03-01
JINGXI XIUMEI BIANCHENG AGRI SCI & TECH CO LTD
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the efficiency of inducing double haploids from a hybrid combination is very low, only less than 10%, and the commonly used doubling agent colchicine is highly toxic to plants and easily causes staining variation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for breeding double haploid of corn*rice distant hybridization
  • Method for breeding double haploid of corn*rice distant hybridization
  • Method for breeding double haploid of corn*rice distant hybridization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for breeding double haploids by distant hybridization of corn×rice, said method comprising the following steps:

[0030] (1) corn ears bagging: choose healthy and disease-free corn plants, put sheepskin bags on the ears before the filaments are drawn out, and wait for the filaments to be drawn out;

[0031] (2) Collecting pollen from rice spikes: after rice heading and before flowering, cut off the young and tender spikelets at the base of each spike, keep the middle and upper spikelets, cover the spikelets with a cowhide bag, and tie the bag tightly. Gently bend the ears of rice, tap the paper bag to make the pollen fall into the bag, tap the paper bag again to make the pollen in the bag concentrate in the middle of the bag, remove the kraft bag, and get the rice pollen for later use;

[0032] (3) Hybridization of corn and rice: step (1) pollination begins on the second day after the filaments are pulled out. The specific pollination method is: first remove th...

Embodiment 2

[0038] A method for breeding double haploids by distant hybridization of corn×rice, said method comprising the following steps:

[0039] (1) corn ears bagging: choose healthy and disease-free corn plants, put sheepskin bags on the ears before the filaments are drawn out, and wait for the filaments to be drawn out;

[0040] (2) Collecting pollen from rice spikes: after rice heading and before flowering, cut off the young and tender spikelets at the base of each spike, keep the middle and upper spikelets, cover the spikelets with a cowhide bag, and tie the bag tightly. After 1.25 hours, put the bagged Gently bend the ears of rice, tap the paper bag to make the pollen fall into the bag, tap the paper bag again to make the pollen in the bag concentrate in the middle of the bag, remove the kraft bag, and get the rice pollen for later use;

[0041] (3) Hybridization of corn and rice: step (1) pollination begins on the 3rd day after the filaments are pulled out. The specific pollinat...

Embodiment 3

[0047] A method for breeding double haploids by distant hybridization of corn×rice, said method comprising the following steps:

[0048] (1) corn ears bagging: choose healthy and disease-free corn plants, put sheepskin bags on the ears before the filaments are drawn out, and wait for the filaments to be drawn out;

[0049] (2) Rice ear pollen collection: after rice earing and before flowering, cut off the young and tender spikelets at the base of each ear, keep the middle and upper spikelets, cover the spikelets with a cowhide bag, and tie the mouth of the bag tightly. After 2 hours, put the bagged Gently bend the ears of rice, tap the paper bag to make the pollen fall into the bag, tap the paper bag again to make the pollen in the bag concentrate in the middle of the bag, remove the kraft bag, and get the rice pollen for later use;

[0050] (3) Hybridization of corn and rice: Step (1) pollination begins on the 4th day when the filaments are pulled out. The specific pollinatio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a plant breeding technology, in particular to a method for breeding a double haploid of corn*rice distant hybridization. The method comprises the steps that a corn hybrid is used as a female parent, a rice variety is used as a male parent, and a corn haploid is obtained by distant hybridization and treated with a chromosome doubling solution to obtain a double haploid cornplant. The method can effectively improve the double haploid induction efficiency, and the application value is large. The invention also provides a brush device which has the functions of measuringa liquid, injecting and brushing by simple modification with a syringe as an original device.

Description

【Technical field】 [0001] The invention belongs to the technical field of plant breeding, in particular to a method for breeding double haploids by distant hybridization of corn and rice. 【Background technique】 [0002] Corn is an annual herbaceous plant of the Poaceae Maize genus. It is an important grain, feed and economical crop in the world. It is widely planted in my country and is one of the three major food crops in my country. It is not only an important food crop, but also an important source of feed and industrial raw materials. It is an important foundation for the development of animal husbandry. It is further processed and utilized to manufacture a wide variety of food, chemical, pharmaceutical, textile, paper and other industrial production. Products, such as corn starch, corn sugar, corn oil, etc. [0003] The sustainable development of corn production and scientific research has great strategic significance for ensuring the food security of the world and Chin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/08
CPCA01H1/02A01H1/08
Inventor 谢宗宜
Owner JINGXI XIUMEI BIANCHENG AGRI SCI & TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com