Method for doubly improving marigold genotype by utilizing chromosomes

A chromosome doubling and marigold technology, applied in the direction of plant genetic improvement, botanical equipment and methods, biochemical equipment and methods, etc., can solve problems such as inability to obtain tetraploid plants, severe vitrification of differentiated seedlings, and complicated operation processes. , to achieve the effect of easy control of environmental conditions, high repeatability, and high doubling efficiency

Inactive Publication Date: 2010-07-07
HUAZHONG AGRI UNIV
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Problems solved by technology

In the process of doubling marigolds by means of tissue culture combined with colchicine chemical mutagenesis, the applicant found that after different concentrations of colchicine were applied to differentiated buds, the vitrification of differentiated seedlings was very serious, and robust tetraploids could not be obtained. plants, and the operation process of tissue culture is relatively complicated and the cost is high

Method used

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  • Method for doubly improving marigold genotype by utilizing chromosomes
  • Method for doubly improving marigold genotype by utilizing chromosomes
  • Method for doubly improving marigold genotype by utilizing chromosomes

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Embodiment 1

[0033] In 2004, the applicant found a marigold male sterile material in the horticultural flower test field of Huazhong Agricultural University. Through multi-generation directional selection, a marigold male sterile dual-purpose line with stable traits was obtained, named M525AB (He Yanhong, et al., Cytological and mapping analysis of a novel male sterile type resulting from spontaneous floral organ homeotic conversion in marigold (Tagetes erecta L.). Molecular Breeding, 2009, DOI: 10.1007 / s11032-009-9372-x. See Springerlink database: http: / / springer.lib.tsinghua.edu.cn / content / 6218771h8502152j / ?p=3a1acfbf8d8442e49f0238dcb63b9da0&pi=0). In the autumn of 2008, the seeds of the male sterile line M525AB of marigold were harvested and stored dry and sealed for use in the next year.

[0034] The marigold doubling method proceeds as follows:

[0035] ①Pregermination treatment: In the first ten days of March, the full seeds of the male sterile line M525AB of marigold were selected ...

Embodiment 2

[0043] This embodiment adopts the shoot tip titration colchicine-induced doubling method, and proceeds according to the following steps:

[0044] ①Pregermination: In early March, select the plump seeds of the male sterile line M525AB of marigold, put them in a petri dish lined with filter paper moistened with water, and cultivate them at 25°C. Seed to the hole tray, the hole tray cultivation substrate is the same as embodiment 1.

[0045] ②Colchicine doubling treatment: As soon as the two cotyledons of the marigold are unfolded, immediately wrap the growth point of the seedlings with absorbent cotton balls, and use colchicine solution with a concentration of 0.025% to 0.4% every 4 hours for stem tip titration, continuous treatment For 2 days, during the treatment process, the top of the plug tray was covered with a sunshade net to keep the cotton balls moist.

[0046] ③Rinse with running water to remove the treatment: After 2 days, remove the cotton ball and rinse the growing...

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Abstract

The invention belongs to the technical field of flower breeding, in particular to a method for doubly improving a marigold genotype by utilizing chromosomes, which is characterized in that male sterile dual-purpose line of a seed or a stem tip of marigold is treated by adopting a colchicine solution so as to enable the number of the chromosomes of the marigold to be doubled, thereby providing a breeding starting material of the improvement of the marigold genotype. The method comprises the following steps of: bud forcing treatment, colchicine doubling treatment, rinsing relief treatment, cultivation management, chromosome ploidy authentication and the like. The preferable treatment is described as follows: applying colchicine solution with concentration of 0.05 percent to marigold germinating seeds and soaking for 3 to 6 hours, and thus, the survival rate of plants is high, and the doubling efficiency also reaches 88.89 percent. The invention can effectively control the environment temperature and humidity when the seeds germinate; the repeatability of a test is high, the operation is convenient and time and labor are saved. The invention is suitable for the improvement of the marigold genotype.

Description

technical field [0001] The invention belongs to the technical field of flower breeding, and in particular relates to a method for improving the genotype of marigolds by using chromosome doubling. Background technique [0002] There are 56 species of plants in the genus Marigold, 27 of which are annual plants. There are three main species of Tagetes widely used at present: Malachite (Tagetes erecta L.), Tagetes erecta L. and triploid Tagetes erecta×Tagetespatula (composed of diploid Tagetes and A triploid sterile line obtained by crossing tetraploid peacock grass). Marigold and peacock grass have the advantages of early flowering, long flowering period, and compact flower shape. They can not only be cultivated in the open field to beautify street scenes and courtyards, but also can be potted to arrange flower beds. At the same time, it is rich in lutein, which is an important source of natural pigment. In addition, they also contain some biologically active ingredients, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01C1/00A01G31/02C12Q1/68
CPCY02P60/21
Inventor 何燕红郭冬胡燕叶要妹包满珠
Owner HUAZHONG AGRI UNIV
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