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Method for rapidly propagating large cherries

A cherry and rapid propagation technology, applied in the field of plant tissue culture, to achieve the effects of obvious proliferation, great practical value, and short embryonic time

Inactive Publication Date: 2016-06-08
SHANDONG INST OF POMOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at present problem, provides a kind of big cherry rapid multiplication method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: a kind of " Zao Daguo " big cherry rapid multiplication method, comprises,

[0020] 1) Bud collection and storage: Take one or more flower buds of the right age at the early stage of uninucleate development that are free from diseases and insect pests and grow well on the inflorescence of large cherry plants on a sunny day, and quickly store the flower buds that have been taken in an ice box;

[0021] 2) Disinfection of anthers: immerse the flower buds stored in the ice box in step 1 in 75% alcohol for disinfection for 15 seconds on an ultra-clean workbench, quickly take them out and immediately immerse them in a saturated sodium hypochlorite solution for disinfection for 10 minutes, and shake gently during the disinfection process The beaker is fully sterilized, and finally rinsed with sterile water several times and then placed on filter paper to absorb the surface moisture of the buds;

[0022] 3) stripping off the anthers in the flower buds, then inocu...

Embodiment 2

[0027] Embodiment two: a kind of " Zao Daguo " big cherry rapid multiplication method, comprises,

[0028] 1) Bud collection and storage: Take one or more flower buds of the right age at the early stage of uninucleate development that are free from diseases and insect pests and grow well on the inflorescence of large cherry plants on a sunny day, and quickly store the flower buds that have been taken in an ice box;

[0029] 2) Disinfection of anthers: immerse the flower buds stored in the ice box in step 1 in 75% alcohol for disinfection for 15 seconds on an ultra-clean workbench, quickly take them out and immediately immerse them in a saturated sodium hypochlorite solution for disinfection for more than 8 minutes, and gently Shake the beaker to make it fully sterilized, and finally rinse it with sterile water several times and put it on filter paper to absorb the surface moisture of the buds;

[0030] 3) stripping off the anthers in the flower buds, then inoculating the strip...

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Abstract

The invention relates to a method for rapidly propagating large cherries. The method includes the steps of selecting early-stage mononuclear anther for disinfecting; inoculating a callus inducing medium, and conducting culturing to obtain calluses; inoculating a differential medium, and conducting culturing to obtain test tube plantlets; inoculating a rooting medium, and conducting screening to obtain monomer plants. The inducing rate of the calluses of cultured large cherry pollen can be 88%, the rooting rate can be 96%, and the transplanting survival rate can be 98%.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for rapid propagation of large cherry. Background technique [0002] Cherry belongs to Rosaceae deciduous arbor fruit trees, and is the earliest fruit tree species of deciduous fruit trees in northern China after Chinese cherry. Therefore, it has long been known as "the first branch of spring fruit". It plays a special role in regulating the off-season of fresh fruit, balancing annual supply and meeting the needs of people's lives. Cherry fruit is rich in nutrients and has certain nutritional value for the human body. According to traditional Chinese medicine, cherry pick has the functions of regulating the middle and replenishing qi and dispelling rheumatism. Large cherry requires less management labor, low production cost, and high economic benefits. It is suitable for cultivation in Liaoning, Shandong, Shaanxi, Henan, Hebei and other places. [0003] With the develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 孙玉刚魏国芹孙杨付全娟杨兴华
Owner SHANDONG INST OF POMOLOGY
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