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Chemical corn double haploid young embryo processing method

A haploid immature embryo and processing method technology, applied in the agricultural field, can solve the problems of insignificant doubling effect, high drug consumption, poor doubling effect and the like

Inactive Publication Date: 2013-04-24
SICHUAN AGRI UNIV
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Problems solved by technology

[0003] With reference to Chen Shaojiang et al., "Corn Haploid Breeding Technology", there are mainly the following disadvantages: 1) the processing time is late, and the doubling effect is poor; 2) the processing concentration is relatively large, and the drug consumption is large; 3) the doubling effect is not obvious

Method used

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Embodiment Construction

[0009] The present invention will be described in detail below in conjunction with specific embodiments.

[0010] 1. Color development of immature embryos and selection of seeds

[0011] The process of inducing the female parent to produce haploid by the inducible line involves the color development process of the top of the grain embryo and the embryo. Only when the immature embryo grows to a certain period of time, the color development will be obvious and sufficient, otherwise the selection and doubling treatment will not be possible.

[0012] In this experiment, samples were first collected and observed 15 days after pollination, and observed every 2 days until the seeds matured. Through observation, we found that 22 days to 25 days after the pollination of the induced line, the corn kernels began to develop color, and until about 28 days, the color development was relatively sufficient, and the haploid seeds and non-haploid seeds could be clearly distinguished. Therefore...

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Abstract

The invention discloses a chemical corn double haploid young embryo processing method. After a haploid is selected to induce pollination of a system for 28 days or so, a haploid kernel young embryo is selected, the selected young embryo is placed in amiprophos-methl to be processed for 2 hours, and processing concentration of propyzamide is under 60 micrometer moles per liter. According to the technology, chemical doubling of the haploid is carried out in early phase, the chemical processing time is shortened, the processing concentration is reduced, consumption of chemicals is reduced, personal injury suffered by processing staff is small, and above all, doubling efficiency of the haploid is improved.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a treatment method for chemically doubling haploid immature embryos of corn. Background technique [0002] Haploid doubling technology is to use chemical and physical methods to change the chromosomes of haploid cells into diploid cells. At present, the chemical doubling method is the most commonly used method, mainly using cell mitotic sorting inhibitors to inhibit the formation of spindle filaments. In maize, current research is mainly focused on doubling maize haploids with colchicine and 4 kinds of herbicides (aquafos-methyl, benzamid, trifluralin and amisulfurin). Doubling methods include soaking buds, soaking seeds and dripping treatment, etc. The factors involved in the corresponding chemical reagent treatment concentration and treatment time are also different, and the doubling effects are also not consistent. [0003] With reference to Chen Shaojiang et al., "Corn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08
Inventor 林海建张志明兰海沈亚欧潘光堂
Owner SICHUAN AGRI UNIV
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