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Asparagus all-male breeding method

A technology of Shi Diao cypress and Shi Diao cypress, applied in the field of Shi Diao cypress all-male breeding, can solve the problems of low differentiation rate of green seedlings, low efficiency, somatic cell interference, etc. Effect

Inactive Publication Date: 2016-09-21
田东县举家富现代农业开发有限公司
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AI Technical Summary

Problems solved by technology

On the whole, the current efficiency of anther culture is not high, and problems such as low callus induction rate, low green seedling differentiation rate, serious interference of somatic cells, and difficulty in rooting of regenerated plants still need to be overcome.

Method used

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Embodiment 1

[0059] In 2010, using the technical route of the present invention, the American variety Apollo and the Dutch variety Shuofeng were selected for all-male breeding. The detailed steps are as follows:

[0060] (1) Selection and pretreatment of anthers

[0061] On April 25, 2012, when the mother stem of the cypress was blooming, at about 9:00 in the morning, the flower buds of 1.5-2.5mm were selected from the Apollo and Shuofengxiong plants, wrapped in wet gauze and brought back to the laboratory, 4 ℃ Low temperature pretreatment;

[0062] (2) Preparation of Anther Induction Medium of Lithia cypress

[0063] Lithia cypress anther induction medium is prepared from the following substances per liter of distilled water:

[0064] Macroelements: KNO 3 2600mg / L, NH 4 NO 3 1700mg / L, KH 2 PO 4 460mg / L, MgSO 4 ∙7H 2 O 370mg / L, CaCl 2 ∙2H 2 O 840mg / L;

[0065] Trace elements: MnSO 4 ∙4H 2 O 22.5mg / L, ZnSO 4 ∙7H 2 O 8.5mg / L, H 3 BO 3 6.25mg / L, KI 0.825mg / L, CuSO 4 ∙5H...

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Abstract

The invention provides an asparagus all-male breeding method. The breeding method comprises the following specific steps: (1) selecting and pretreating anther; (2) preparing an asparagus anther induction medium; (3) performing high sugar treatment on the anther, then disinfecting and inoculating; (4) performing induction culture on the anther; (5) performing callus differentiation culture; (6) enabling green buds to expand propagation, multiplication and root; (7) hardening seedlings and transplanting; (8) observing chromosome ploidy, removing non-diploid plants, and distinguishing super male plants and female plants; (9) screening and hybridizing asparagus super male plants and female plants. According to the all-male breeding method, asparagus anther culture plants are obtained through an efficient anther culture way, the diploid super male plants and female plants are obtained after the non-diploid plants are removed, further the asparagus super male plants and female plants with excellent agronomic characters are selected for hybridization, thus obtaining a high-yield and high-quality F1 generation all-male system high in growth rate and strong in disease resistance. The asparagus super male plants and female plants are high in inductivity and fewer in abnormal plant, and the obtained all-male system is excellent in quality and great in industry value.

Description

technical field [0001] The present invention relates to the field of agricultural high-tech, in particular to a method of cultivating diploid supermale and female plants of cypress cypress through the high-efficiency flower culture method, screening super male and female plants of cypress cypress with excellent agronomic properties, and hybridizing them to obtain excellent F1 generation whole plants. All-male breeding method for the male line of Shi Diaobo. Background technique [0002] Shi Diaobo ( Asparagus officinalis L.), also known as asparagus, asparagus, and asparagus, is a perennial herbaceous plant of the genus Asparagus in the family Liliaceae. It is a dioecious plant, and its edible parts are mainly pumped tender stems. The tender stems of asparagus are delicate in texture, soft and delicious in fiber, delicious in flavor and unique in aroma. It is a health-care vegetable with high nutritional value. King of Vegetables". [0003] Asparagus tender stems are ric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 王纪芝黎建波陈丁龙
Owner 田东县举家富现代农业开发有限公司
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