Asparagus all-male breeding method
A technology of Shi Diao cypress and Shi Diao cypress, applied in the field of Shi Diao cypress all-male breeding, can solve the problems of low differentiation rate of green seedlings, low efficiency, somatic cell interference, etc. Effect
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[0059] In 2010, using the technical route of the present invention, the American variety Apollo and the Dutch variety Shuofeng were selected for all-male breeding. The detailed steps are as follows:
[0060] (1) Selection and pretreatment of anthers
[0061] On April 25, 2012, when the mother stem of the cypress was blooming, at about 9:00 in the morning, the flower buds of 1.5-2.5mm were selected from the Apollo and Shuofengxiong plants, wrapped in wet gauze and brought back to the laboratory, 4 ℃ Low temperature pretreatment;
[0062] (2) Preparation of Anther Induction Medium of Lithia cypress
[0063] Lithia cypress anther induction medium is prepared from the following substances per liter of distilled water:
[0064] Macroelements: KNO 3 2600mg / L, NH 4 NO 3 1700mg / L, KH 2 PO 4 460mg / L, MgSO 4 ∙7H 2 O 370mg / L, CaCl 2 ∙2H 2 O 840mg / L;
[0065] Trace elements: MnSO 4 ∙4H 2 O 22.5mg / L, ZnSO 4 ∙7H 2 O 8.5mg / L, H 3 BO 3 6.25mg / L, KI 0.825mg / L, CuSO 4 ∙5H...
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