A kind of all-male breeding method of Cypress chinensis

Abstract

The invention provides an asparagus all-male breeding method. The breeding method comprises the following specific steps: (1) selecting and pretreating anther; (2) preparing an asparagus anther induction medium; (3) performing high sugar treatment on the anther, then disinfecting and inoculating; (4) performing induction culture on the anther; (5) performing callus differentiation culture; (6) enabling green buds to expand propagation, multiplication and root; (7) hardening seedlings and transplanting; (8) observing chromosome ploidy, removing non-diploid plants, and distinguishing super male plants and female plants; (9) screening and hybridizing asparagus super male plants and female plants. According to the all-male breeding method, asparagus anther culture plants are obtained through an efficient anther culture way, the diploid super male plants and female plants are obtained after the non-diploid plants are removed, further the asparagus super male plants and female plants with excellent agronomic characters are selected for hybridization, thus obtaining a high-yield and high-quality F1 generation all-male system high in growth rate and strong in disease resistance. The asparagus super male plants and female plants are high in inductivity and fewer in abnormal plant, and the obtained all-male system is excellent in quality and great in industry value.

Description

technical field [0001] The present invention relates to the field of agricultural high-tech, in particular to a method of cultivating diploid supermale and female plants of cypress cypress through the high-efficiency flower culture method, screening super male and female plants of cypress cypress with excellent agronomic properties, and hybridizing them to obtain excellent F1 generation whole plants. All-male breeding method for the male line of Shi Diaobo. Background technique [0002] Shi Diaobo ( Asparagus officinalis L.), also known as asparagus, asparagus, and asparagus, is a perennial herbaceous plant of the genus Asparagus in the family Liliaceae. It is a dioecious plant, and its edible parts are mainly pumped tender stems. The tender stems of asparagus are delicate in texture, soft and delicious in fiber, delicious in flavor and unique in aroma. It is a health-care vegetable with high nutritional value. King of Vegetables". [0003] Asparagus tender stems are ric...

AI Technical Summary

Problems solved by technology

On the whole, the current efficiency of anther culture is not high, and problems such as low callus induction rate, low green seedling differentiation rate, serious interference of somatic cells, and difficulty in rooting of regenerated plants still need to be overcome.

Images