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Method for improving corn haploid doubling efficiency

A haploid and corn technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve problems affecting doubling efficiency and scale, reduced seedling survival rate, and reduced breeding efficiency, so as to reduce breeding costs , Reduce the dosage of medicine, and the effect of easy operation

Pending Publication Date: 2021-03-30
JILIN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method greatly reduces the survival rate of seedlings, and the procedures are complicated, which increases the cost of a lot of labor, chemical reagents, and materials (seedling trays, matrix soil, iron frames, etc.), affects the doubling efficiency and scale, and reduces the breeding efficiency.

Method used

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  • Method for improving corn haploid doubling efficiency
  • Method for improving corn haploid doubling efficiency
  • Method for improving corn haploid doubling efficiency

Examples

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Effect test

Embodiment 1

[0038] A method for improving the doubling efficiency of corn haploid, the specific operation is as follows:

[0039] (1) Preparatory work

[0040] Operators wore gas masks and prepared a mixed solution of chemical doubling agents colchicine solution (0.02%) and thilone (2%) in a laboratory fume hood. Prepare the fine sand soil for germination and place it flat in the germination tray ( figure 1 ), the thickness of the sandy soil is about 2.0-2.5cm, and the sandy soil is sprayed and sterilized with a solution of fenzamid (2%).

[0041] (2) Seed soaking

[0042] Maize haploid seeds induced by Jidan 27 and other 4 populations (Table 1) were soaked in distilled water for 4-6h.

[0043] (3) Germinate and grow seedlings

[0044] Take out the soaked seeds, put the germ face up and staggeredly on the sandy soil plane of the germination tray prepared in advance (the distance between each seed is about 3-5cm). After the seeds are neatly placed, press them into the sand and cover t...

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Abstract

The invention discloses a method for improving corn haploid doubling efficiency, and belongs to the technical field of crop breeding. The method, disclosed by the invention, for improving corn haploiddoubling efficiency comprises the steps of sandy soil disinfection, seed soaking, germination and seedling raising, sand removal and seedling taking, medicament soaking, flushing and direct transplanting into a field. Medicament soaking is carried out when seedlings grow to 2-3 leaves, the doubling efficiency of corn haploid is higher, tedious steps of seedling raising pot preparation, bud cutting, greenhouse seedling recovering and the like in a conventional medicament bud soaking doubling method are omitted, the use amount of chemical agents is reduced, the breeding cost is greatly reduced,the haploid breeding efficiency is improved, and the method is easy and convenient to operate and can be popularized and applied on a large scale.

Description

technical field [0001] The invention relates to the technical field of crop breeding, in particular to a method for improving the doubling efficiency of maize haploids. Background technique [0002] In recent years, the maize haploid breeding technology based on biological induction has gradually become the key technology of maize breeding. Double haploid (DH) technology is the fastest, most convenient, economical, practical and direct method for breeding maize inbred lines. Its basic process is to obtain homozygous diploid plants through artificial or natural doubling of biologically induced haploid plants, and then select superior lines from them. Its main feature is that the line selection is fast, and a stable pure line can be obtained in only 2 generations (traditional inbred line selection requires more than 7 generations). [0003] With the breeding of new induction lines (Nongda Gaoyu No. 1, Jigaoyu No. 3, and Nongda Gaoyu No. 5), the efficiency of induction and id...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/20A01G7/06A01H1/08
CPCA01G22/20A01G7/06A01H1/08
Inventor 刘文国岳尧海路明周旭东王敏张建新马英杰张志军周德龙郑淑波孟令聪高嵩何欢王绍平卢实邢跃先王楠
Owner JILIN ACAD OF AGRI SCI
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