Chromosome doubling method for pepper or eggplant haploid plant

A chromosome doubling and haploid technology, applied in the field of plant tissue culture, can solve the problems of low success rate of one-time doubling, time-consuming and labor-intensive

Active Publication Date: 2020-07-07
北京市海淀区植物组织培养技术实验室 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is time-consuming and laborious, and the success rate of one doubling is low, and the success rate of one doubling of pepper and eggplant is generally 0-30%.

Method used

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  • Chromosome doubling method for pepper or eggplant haploid plant
  • Chromosome doubling method for pepper or eggplant haploid plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Chromosome doubling method of pepper haploid plants:

[0026] Pepper flower buds whose pollen development period is mononucleate and marginal stage were retrieved from the planting shed, surface-sterilized with 10% sodium hypochlorite for 20 minutes under aseptic conditions, and rinsed with sterile water for 3 times. The anthers were stripped off and inoculated in the induction medium (MS basic medium added with 0.1mg / L BA, 0.1mg / L IAA, 0.01mg / L NAA) to obtain embryoid bodies, and the embryoid bodies were transferred to the Grow into plantlets in the MS basic medium of the growth regulator; then transfer the plantlets to the rooting and strong seedling medium (MS basic medium supplemented with IBA 1mg / L), and finally obtain the regenerated plants of pepper pollen;

[0027] Select 30 pepper pollen regenerated plants with a plant height of 3cm to 5cm and 3 to 4 leaves (measured as haploid plants by flow cytometry), remove the large leaves, keep the root syste...

Embodiment 2

[0032] Example 2 : Eggplant haploid plant chromosome doubling method:

[0033] Eggplant flower buds whose pollen development period was mononucleate and marginal stage were retrieved from the greenhouse, and surface-sterilized with 10% sodium hypochlorite for 10 minutes under aseptic conditions, and rinsed with sterile water for 3 times. The anthers were stripped off and inoculated in the induction medium (MS basic medium added with 0.1mg / L~1mg / L KT, 0.1mg / L~1mg / L IAA, 0.01mg / L~0.05mg / L NAA, 0.1%~ 0.3% activated carbon) to obtain embryoid bodies, the embryoid bodies are transferred to the MS0 medium without any growth regulators, and grow into plantlets; then the plantlets are transferred to rooting and strong seedling medium (MS basic medium Add IBA 1mg / L), finally obtain eggplant pollen regenerated plants;

[0034] Select 40 eggplant regenerated plants with a plant height of 3cm-5cm and 3-4 leaves (determined as haploid plants by flow cytometry), remove the large leaves a...

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Abstract

The invention provides a chromosome doubling method for a pepper or eggplant haploid plant, and belongs to the technical field of plant tissue culture. The method comprises the following steps: addinga sterile colchicine solution and a sterile DMSO into a sterile culture bottle, retaining or removing roots of an anther culture regenerated plant of pepper or eggplant, placing the anther culture regenerated plant into the sterile culture bottle, and performing shaking seedling-soaking treatment; and after treatment for 24 h-48 h, taking out the anther culture regenerated plant, transferring theanther culture regenerated plant to a MS basic culture medium for light culture to obtain a diploid plant after culture for one month. The method of the invention has the following advantages: (1) the method can ensure that all growth points on the plant are treated; (2) the method can ensure that the growth points of the plant are always maintained with a treatment solution during treatment, buta use amount of the treatment solution is less, and the cost is reduced; and (3) a doubling success rate is 60%-100%.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a chromosome doubling method for pepper or eggplant haploid plants. Background technique [0002] Haploid breeding is one of the methods of plant breeding. Haploid plants can be induced by anther culture, and then treated with artificial means such as colchicine to double the chromosome group, so that the plants can restore the normal number of chromosomes and become fertile pure plants. Diploid (DH) is directly applied to breeding, shortening the cycle and greatly accelerating the breeding process. [0003] The establishment of DH population is the key technology of haploid breeding, including haploid induction and chromosome doubling. At present, plant tissue culture technology is mainly used to obtain haploid plants through androgenic pathways such as anther or microspore culture and gynogenetic pathways such as cultivating unpollinated ovaries and ov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/001A01H4/008
Inventor 邓晓梅张秦惠志明张树根张军民李春玲
Owner 北京市海淀区植物组织培养技术实验室
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