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Ornithogalum arabicum Linn. tissue culture rapid breeding method

A white-flowered tiger eye and tissue culture technology, applied in the field of plant propagation, can solve the problems of difficult regeneration and low reproductive coefficient of bulbs, and achieve the effect of simple operation, high multiplication coefficient, and sustainable production and development

Active Publication Date: 2016-11-09
四川天艺优境环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, this application aims at the problem of difficult regeneration and low bulb propagation coefficient when using underground corm propagation, and provides a tissue culture rapid propagation method for Dieffenbachia albicans, which has the remarkable advantages of easy operation and high multiplication coefficient, and can be used Realize the factory-scale production of high-quality seedlings

Method used

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  • Ornithogalum arabicum Linn. tissue culture rapid breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Step 1, selection and processing of explants

[0046] Selecting the leaves of Dieffenbachia punctatus as a starting material, the collected leaves are cut into 3cm leaf segments as explants for tissue culture;

[0047] Step 2, the leaves are induced to form seedlings

[0048] Step 2-1, transfer the surface-sterilized leaf segments to a sterile inoculation tray, and dry the surface moisture of the leaf segments with sterile filter paper;

[0049] Step 2-2, place the sterilized leaf segment flat or insert it into the bulbil induction medium MS+TDZ2.0mg / L to induce bulbils, the culture temperature is 25°C, the light time is 14h / d, and the light intensity is 40u.Mol. m -2 .s -1 ; After a complete root system is gradually formed on the surface of the blade, the root system is inserted into the medium to become a new seedling;

[0050] The basic medium used is MS medium, the dosage of sucrose is 30g / L, the coagulant is agar powder, the dosage is 6.5g / L, and the pH of the ...

Embodiment 2

[0054] Step 1, selection and processing of explants

[0055] Selecting the leaves of Dieffenbachia vinifera as starting material, the collected leaves are cut into 2cm leaf segments as explants for tissue culture;

[0056] Step 2, the leaves are induced to form seedlings

[0057] Step 2-1, transfer the surface-sterilized leaf segments to a sterile inoculation tray, and dry the surface moisture of the leaf segments with sterile filter paper;

[0058] Step 2-2, place the sterilized leaf segment flat or insert it into the bulbil induction medium MS+TDZ2.0mg / L to induce bulbils, the culture temperature is 25°C, the light time is 13h / d, and the light intensity is 50u.Mol. m -2 .s -1 ; After a complete root system is gradually formed on the surface of the blade, the root system is inserted into the medium to become a new seedling;

[0059] The basic medium used is MS medium, the dosage of sucrose is 20g / L, the coagulant is agar powder, the dosage is 6g / L, and the pH of the mediu...

Embodiment 3

[0063] Step 1, selection and processing of explants

[0064] Selecting the leaves of Dieffenbachia vinifera as a starting material, the collected leaves are cut into 4cm leaf segments as explants for tissue culture;

[0065] Step 2, the leaves are induced to form seedlings

[0066] Step 2-1, transfer the surface-sterilized leaf segments to a sterile inoculation tray, and dry the surface moisture of the leaf segments with sterile filter paper;

[0067] Step 2-2, lay the sterilized leaf segments flat or insert them into the bulbil induction medium MS+TDZ2.0mg / L to induce bulbils, the culture temperature is 25°C, the light time is 12h / d, and the light intensity is 60u.Mol. m -2 .s -1 ; After a complete root system is gradually formed on the surface of the blade, the root system is inserted into the medium to become a new seedling;

[0068] The basic medium used is MS medium, the dosage of sucrose is 40g / L, the coagulant is agar powder, the dosage is 7g / L, and the pH of the me...

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Abstract

The invention discloses an Ornithogalum arabicum Linn. tissue culture rapid breeding method. The method comprises selecting leaves of Ornithogalum arabicum Linn. as explants for tissue culture, carrying out leaf surface bulbil induced culture to obtain a seedling, or leaf callus induced culture, adventitious bud induced culture and rooting culture to obtain a seedling, carrying out tissue culture seedling acclimatizating and transplanting after the seedling produces adventitious root, and then carrying out normal culture. The method solves the problem that the underground bulb breeding method has regeneration difficulty and a low seedball propagation coefficient, has the advantages of operation simpleness and high propagation coefficient, is not limited by regions, seasons and climate and realizes industrial large-scale production of high-quality seedlings.

Description

technical field [0001] The application belongs to the technical field of plant propagation, and in particular relates to a method for rapid propagation of Dieffenbachia albicans through tissue culture. Background technique [0002] Ornithogalum thyroides is a perennial bulbous flower of the genus Ornithogalum spp in the family Liliaceae. It is 25-50 cm high and has round and smooth bulbs with high ornamental value. The leaves are clustered at the base, lanceolate and long, with long tails at the top, dark green, and sessile. The flowers are small, the racemes are compact, pyramid-shaped, born on the top of the scape drawn from the leaves, more than 25 small flowers, the flower shape is cup-shaped, the flower color is white, and there is no green stripe in the center of the petals. [0003] Not only its flowers and bulbs have high ornamental value, but can be widely used in landscaping and as fresh cut flowers, and chemical composition analysis shows that it contains seconda...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 姜福星陈其兵黄远祥周鹏
Owner 四川天艺优境环境科技有限公司
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