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Tissue culture and rapid propagation method of didymostigma obtusum

A technology of two-leaved lettuce and tissue culture, which is applied in the field of plant propagation and can solve problems that have not been reported

Active Publication Date: 2013-11-06
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are no reports on the technology of tissue culture and rapid propagation of Lemonia dichotome at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] a. Disinfection treatment of explants: Use the leaves of the wild Didymostigma obtusum (Clarke) W.T.Wang plant in Nankun Mountain, Shihe Wonderland Scenic Area, Guangdong Province as explants, rinse the explants with clean water first, and then use Mass fraction 1%NaClO solution was sterilized for 10 minutes, rinsed with sterile water 5 times, and the explants after disinfection were obtained;

[0018] b. Induction of the first generation of adventitious buds: inoculate the sterilized explants on the induction medium, and inoculate them at a temperature of 25±1°C and a light intensity of 50 μmol·m -2 ·s -1 , light time 12h·d -1 Under the condition of culturing for 30 days, the leaf explants were induced to form adventitious buds. The induction medium used was: TDZ2.0μmol, NAA0.2μmol, sucrose 30g, agar 5.5g per liter, and the rest was MS medium, pH 5.6, the induction coefficient is 44.6, and the induction medium per liter is prepared as follows: dissolve 2.0 μmol of TD...

Embodiment 2

[0024] a. Disinfection treatment of explants: Use the leaves of the wild Didymostigma obtusum (Clarke) W.T.Wang plant in Nankun Mountain, Shihe Wonderland Scenic Area, Guangdong Province as explants, rinse the explants with clean water first, and then use Mass fraction 1%NaClO solution was sterilized for 11 minutes, rinsed with sterile water 5 times, and the explants after disinfection were obtained;

[0025] b. Induction of the first generation of adventitious buds: inoculate the sterilized explants on the induction medium, and inoculate them at a temperature of 25±1°C and a light intensity of 50 μmol·m -2 ·s -1 , light time 12h·d -1 Under the condition of culturing for 30 days, the leaf explants were induced to form adventitious buds. The induction medium used was: each liter contained TDZ3. 5.6, each liter of induction medium is prepared as follows: dissolve 2.0 μmol of TDZ, 0.2 μmol of NAA, 30 g of sucrose, and 5.5 g of agar in 1L of MS medium, and sterilize at 121°C for...

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Abstract

The invention discloses a tissue culture and rapid propagation method of didymostigma obtusum. According to the tissue culture and rapid propagation method of the didymostigma obtusum, leaves of the didymostigma obtusum are utilized as explants to achieve rapid propagation of seedlings of the didymostigma obtusum which belongs to rare ornamental plants through screening and optimization of the formula and culture conditions of culture medium primary generation, transgenerational proliferation, rooting and transplanting and through the steps of primary generation, transgenerational proliferation and rooting culture of adventitious buds and transplanting of test-tube seedlings. Generally only 110 days are required to obtain the seedlings of the didymostigma obtusum and the survival rate of the seedlings of the didymostigma obtusum can reach more than 95%. Accordingly, the tissue culture and rapid propagation method of the didymostigma obtusum has the advantages of being simple, rapid and effective and directly applicable to breeding and protection of the didymostigma obtusum and accordingly laying a foundation for genetic improvement and protection, development and utilization of germplasm resources of the species.

Description

Technical field: [0001] The invention belongs to the field of plant propagation, and in particular relates to a method for tissue culture and rapid propagation of Lemonia dichotome. Background technique: [0002] Didymostigma obtusum (Clarke) W.T.Wang is a perennial herb of Gesneriaceae Didymostigma obtusum, distributed in Fujian, Guangdong, Guangxi and Hainan. It likes to grow by shaded streams and is not common in the wild. The number of populations is small, and it is included in "Rare Plants in China" (Xing Fuwu, 2005). The leaves of the two-leaf lettuce have soft hairs on both sides, the back of the leaves is purple-red, and the flowers are blue-purple. They have high ornamental value and are suitable for planting. Ground cover ornamental plants. [0003] There are no reports on the tissue culture and rapid propagation techniques of Lemonia dichotome at home and abroad. Invention content: [0004] The purpose of the present invention is to provide a high survival ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨国陈红锋马国华
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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