Set of paddy rice anti-brown-planthopper genes, coded protein thereof, and application thereof

A technology against brown planthoppers and rice, applied in the field of genetic engineering, can solve the problems that the effect has not been reported yet

Active Publication Date: 2013-07-24
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of such protein kinases in rice resistance to BPH has not been reported

Method used

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  • Set of paddy rice anti-brown-planthopper genes, coded protein thereof, and application thereof
  • Set of paddy rice anti-brown-planthopper genes, coded protein thereof, and application thereof
  • Set of paddy rice anti-brown-planthopper genes, coded protein thereof, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1. Discovery of rice resistance to brown planthopper-related proteins and their coding genes

[0043] 1. Resistance performance of rice variety Rathu Heenati to brown planthopper

[0044] Rice variety Rathu Heenati is highly resistant to brown planthopper. After being inoculated with N. lugens nymphs, Rathu Heenati showed normal plant growth and no obvious adverse symptoms. And the susceptible control 02428 showed curled leaves, and the plants wilted until dead (see figure 1 ).

[0045] 2. Anti-BPH gene mapping

[0046] 1. Preliminary mapping of the brown planthopper resistance gene

[0047] Detection of brown planthopper resistance genes using Rathu Heenati / 02428 hybrid F2 population. A main gene for resistance to BPH was detected in the interval of two SSR markers RM8213 and RM5953 on the short arm of chromosome 4, and named Bph17.

[0048] 2. Fine mapping of brown planthopper resistance genes

[0049] According to the results of preliminary mapping, u...

Embodiment 2

[0062] Embodiment 2, the acquisition and identification of transgenic plants

[0063] 1. Agrobacterium-mediated transformation

[0064] Using Agrobacterium strain EHA105 (purchased from Handsome Company, USA) as the mediator, the four gene recombination vectors constructed above were introduced into the susceptible species Kitaake.

[0065] (1) Cultivate Agrobacterium containing recombinant Bph17 for 16 hr at 28°C, collect the cells, and dilute them into N6 liquid medium containing 100 μmol / L to a concentration of OD 600 ≈0.5, to obtain the bacterial liquid;

[0066] (2) Mix and infect the mature embryogenic callus of rice that has been cultivated for one month with the above-mentioned bacterial solution for 30 minutes, blot the bacterial solution with filter paper, and then transfer to the co-cultivation medium (N6 solid co-cultivation medium, purchased by Sigma Company) medium, 24°C for 3 days;

[0067] (3) Inoculate the above callus on N6 solid selection medium containin...

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Abstract

The invention belongs to the technical field of gene engineering, and specifically relates to a set of paddy rice anti-brown-planthopper genes, and coded proteins thereof and an application thereof. The sequences of the paddy rice anti-brown-planthopper genes are represented by SEQ ID No.5-No.8. The sequences of the coded proteins are represented by SEQ ID No.1-No. 4. The paddy insect-resistance-related proteins influence the insect resistance of plant. The increase of the protein-coding gene can lead to insect resistance of susceptible plants, such that anti-brown-planthopper transgenic plants can be cultivated. The gene and the coded protein thereof can be used in plant genetic improvement.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a group of rice brown planthopper resistance genes, encoded proteins and applications thereof. technical background [0002] Rice is one of the most important food crops in the world, but it is always harmed by various diseases and insect pests in the process of rice production. In my country, the annual loss of rice production due to pests and diseases reaches 4-5 million tons. Brown planthopper is one of the most important pests of rice. It has the characteristics of short growth cycle, strong fecundity and high migration, and poses a great threat to rice production. For a long time, chemical control has played an important role in reducing the harm of brown planthoppers. However, the use of chemical pesticides not only made brown planthoppers resistant to pesticides, but also indirectly killed the natural enemies of brown planthoppers, which eventually ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/12C12N15/54C12N15/63C12N15/11C12N5/10C12N1/21C12N15/82C12N15/84A01H5/00
Inventor 万建民刘裕强吴寒江玲刘艳玲何俊陈亮明刘世家刘喜王益华赵志刚张文伟陈赛华
Owner NANJING AGRICULTURAL UNIVERSITY
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