SNP marker for determining and/or inheriting growth traits of modified pigs

A technology of marking and breeding pigs, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve the problems of identifying main genes and molecular markers, etc.

Active Publication Date: 2017-09-15
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the live pigs sold in my country's market are Du long-term three-way hybrid pigs, but so far, the mai

Method used

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  • SNP marker for determining and/or inheriting growth traits of modified pigs
  • SNP marker for determining and/or inheriting growth traits of modified pigs
  • SNP marker for determining and/or inheriting growth traits of modified pigs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0084] In this example, in order to verify the effect of the top 14 SNP markers obtained in Example 1, the significance of the association was expanded to a total of 1847 Duchangda commercial pigs for genetic testing. Although these 1847 individuals came from groups of different periods and different pig farms, the results of the association analysis found that the association between rs16806345 and body length (P value) reached 3.082E-23; even if the rs17472686 locus is associated with body length ( P value) also reached 2.45E-12 (Table 3). In a biostatistical sense, when the correlation between SNP locus and phenotype reaches 1×10 -5 It is believed that there is a correlation between this locus and the phenotype. It can be seen that this result shows that the 14 SNP markers such as rs16806345 locus have a very significant association with pig carcass length, and the favorable allele A at rs16806345 locus can increase about The 2.41cm carcass is straight and its frequency in D...

Embodiment 3

[0086] Taking the detection of the rs16806345 locus as an example, the correlation between one of the 14 tightly linked SNP markers in Example 2 and the growth traits of pigs was evaluated.

[0087] The applicant then tested the separation status of the rs16806345 locus in Landrace and Dabai commercial purebred pigs and evaluated its effect on their body length, so as to provide theoretical and technical support for the molecular selection of growth traits of these two breeds.

[0088] The rs16806345 site-specific detection probe (sequence: FAM-TAGTACACAGTGGCCCCACTTC-MGB, VIC-TAGTACACAGTAGCCCCACTTC-MGB) was used to genotype 297 purebred Landraceae and 163 purebred Landraceae samples, and found that the site is advantageous The frequency of alleles in the large white is only 9.9%, but the correlation between the phenotype and the gene is still extremely significant (P=0.00164, Table 5); and the frequency of the favorable allele in the large white reaches 63.8%, and its genotype and t...

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Abstract

The invention relates to an SNP marker for determining and/or inheriting growth traits of modified pigs. The SNP marker comprises at least one of 14 SNP markers respectively positioned at a No. 16806345 site, a No. 16940077 site, a No. 17473931 site, a No. 17013787 site, a No. 17096014 site, a No. 17007981 site, a No. 16916995 site, a No. 17102194 site, a No. 17114026 site, a No. 16682972 site, a No. 16765047 site, a No. 16788727 site, a No. 16767555 site and a No. 17472686 site on a No. chromosome of a 10.2 edition of international swine genome from the 5' end.

Description

Technical field [0001] This application relates to a SNP marker for determining and / or genetically improving pig growth traits. Background technique [0002] Growth traits are an important indicator of pig economic traits, so it is an important production trait in breeding pig genetic improvement research. It mainly includes pig carcass length, carcass oblique length, carcass weight, chest and back fat thickness, and cervical spine total length. Many kinds of weights. The traditional method of pig breeding is to eliminate "unqualified" breeding pigs through manual measurement of pig breeds and visual observation. This traditional breeding method has certain results, but it is easy to mistakenly eliminate the really valuable breeding pigs, and the continuity is not strong, and there is no long-term plan. The genetic improvement method based on the molecular markers of major genes is scientific, long-term and economical, and is a necessary supplement to traditional pig breeding. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 黄路生麻骏武李景彭颂钟烈鹏周李生杨斌
Owner JIANGXI AGRICULTURAL UNIVERSITY
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