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A SNP marker for measuring and/or genetically improving pig growth traits

A technology for growth traits and markers, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve problems such as the identification of major genes and molecular markers.

Active Publication Date: 2020-06-26
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the live pigs sold in my country's market are Du long-term three-way hybrid pigs, but so far, the main genes and molecular markers related to their growth traits have not been identified through scientific and technological means

Method used

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  • A SNP marker for measuring and/or genetically improving pig growth traits
  • A SNP marker for measuring and/or genetically improving pig growth traits
  • A SNP marker for measuring and/or genetically improving pig growth traits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0084] In this example, in order to verify the effects of the top 14 SNP markers with the highest degree of association significance obtained in Example 1, they were expanded to a total of 1847 Dudu long commercial pig populations for genetic testing. Although these 1847 individuals came from different periods and different pig farms, the results of the association analysis found that the correlation between rs16806345 and body length (P value) reached 3.082E-23; P value) also reached 2.45E-12 (Table 3). In the biostatistical sense, when the correlation between the SNP site and the phenotype reaches 1×10 -5 , it is considered that there is a correlation between this locus and the phenotype. It can be seen that the results show that 14 SNP markers such as rs16806345 locus are extremely significantly correlated with pig carcass length, and the favorable allele A at the rs16806345 locus, for example, can increase about The carcass length of 2.41cm is only 18.28% in Du long pigs ...

Embodiment 3

[0086] Taking the detection of rs16806345 as an example, the correlation between one of the 14 closely linked SNP markers in Example 2 and the growth traits of pigs was evaluated.

[0087] The applicant then tested the segregation status of the rs16806345 locus in Landrace and Large White commercial purebred pigs and evaluated its impact on their body length, in order to provide theoretical and technical support for the molecular selection of growth traits of these two breeds.

[0088] After using the rs16806345 site-specific detection probe (sequence: FAM-TAGTACACAGTGGCCCCACTTC-MGB, VIC-TAGTACACAGTAGCCCCACTTC-MGB) to perform genotyping detection on 297 purebred Large White and 163 purebred Landrace samples, it was found that this locus is favorable, etc. The frequency of the allele in Large White was only 9.9%, but the correlation between phenotype and gene was still extremely significant (P=0.00164, Table 5); while the frequency of favorable allele in Landrace was 63.8%, the ...

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Abstract

The invention relates to an SNP marker for determining and / or inheriting growth traits of modified pigs. The SNP marker comprises at least one of 14 SNP markers respectively positioned at a No. 16806345 site, a No. 16940077 site, a No. 17473931 site, a No. 17013787 site, a No. 17096014 site, a No. 17007981 site, a No. 16916995 site, a No. 17102194 site, a No. 17114026 site, a No. 16682972 site, a No. 16765047 site, a No. 16788727 site, a No. 16767555 site and a No. 17472686 site on a No. chromosome of a 10.2 edition of international swine genome from the 5' end.

Description

technical field [0001] The present application relates to a SNP marker for measuring and / or genetically improving pig growth traits. Background technique [0002] Growth traits are an important indicator of economic traits of pigs, so they are important production traits in the genetic improvement of breeding pigs. It mainly includes one or more of pig carcass length, carcass slant length, carcass weight, breast and back fat thickness, and total cervical spine length. Various weights and so on. The traditional pig breeding method is to eliminate "unqualified" breeding pigs after manual measurement and naked eye observation of the pig breeds. This traditional breeding method has achieved certain results, but it is easy to mistakenly eliminate truly valuable breeding pigs, and the continuity is not strong, and there is no long-term planning. The genetic improvement method based on the main gene molecular marker is scientific, long-term, economical and fast, and is a necessar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 黄路生麻骏武李景彭颂钟烈鹏周李生杨斌
Owner JIANGXI AGRICULTURAL UNIVERSITY
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