SNP marker affecting content of guanine in individual pigs
A technology for marking and breeding pigs, used in the direction of stably introducing foreign DNA into chromosomes, recombinant DNA technology, and microbial determination/inspection, etc.
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[0083] 1. Acquisition, quality control and typing of pig whole genome resequencing data
[0084] Randomly select 421 heads from the F6 population, 665 random selections from the F7 population, a total of 1086 heads, collect a small ear tissue sample from each individual, and extract the genomic DNA of each individual with the standard phenol-chloroform method. The extracted genomic DNA was dissolved in TE buffer. Use the Nanodrop-ND1000 spectrophotometer to test the quality of the extracted genomic DNA. When the ratio of A260 / 280 is 1.8-2.0 and the ratio of A260 / 230 is about 1.7-1.9, the quality standard is reached.
[0085] Dilute the concentration of DNA samples that meet the standards to 50ng / μl, and use the HiSeq X Ten sequencer platform of Illumina to perform low-depth resequencing on each DNA sample (the average sequencing depth is about 7.8X), and all the double-end reads obtained The long (read) is mapped to the 11.1 version of the international pig genome using BWA s...
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