SNP marker affecting content of guanine in individual pigs

A technology for marking and breeding pigs, used in the direction of stably introducing foreign DNA into chromosomes, recombinant DNA technology, and microbial determination/inspection, etc.

Active Publication Date: 2020-09-25
JIANGXI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the research on improving the taste quality of pork has mainly focused on the intramuscular fat, tenderness, and juicin

Method used

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  • SNP marker affecting content of guanine in individual pigs
  • SNP marker affecting content of guanine in individual pigs
  • SNP marker affecting content of guanine in individual pigs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] 1. Acquisition, quality control and typing of pig whole genome resequencing data

[0084] Randomly select 421 heads from the F6 population, 665 random selections from the F7 population, a total of 1086 heads, collect a small ear tissue sample from each individual, and extract the genomic DNA of each individual with the standard phenol-chloroform method. The extracted genomic DNA was dissolved in TE buffer. Use the Nanodrop-ND1000 spectrophotometer to test the quality of the extracted genomic DNA. When the ratio of A260 / 280 is 1.8-2.0 and the ratio of A260 / 230 is about 1.7-1.9, the quality standard is reached.

[0085] Dilute the concentration of DNA samples that meet the standards to 50ng / μl, and use the HiSeq X Ten sequencer platform of Illumina to perform low-depth resequencing on each DNA sample (the average sequencing depth is about 7.8X), and all the double-end reads obtained The long (read) is mapped to the 11.1 version of the international pig genome using BWA s...

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Abstract

The invention provides a SNP marker affecting the content of guanine in pork. The three most significant SNP markers are: (I) the SNP marker on nucleic acid I, located at a 55495763 site from the5'end on a chromosome No.12 of a 11.1 version international porcine genome, is G or A; (II) the SNP marker on nucleic acid II, located at a 55500274 site from the 5'end on the chromosome No.12 of the 11.1 version international porcine genome, is C or CT; and (III) the SNP marker on nucleic acid III, located at a 54924843 site from the 5'end on the chromosome No.12 of the 11.1 version international porcine genome, is T or C.

Description

technical field [0001] The invention provides a SNP marker that affects the content of guanine in individual pigs. Background technique [0002] For thousands of years, pork has played a very important role in the daily diet of Chinese residents. As the main source of animal protein intake, pork can provide the human body with a variety of essential amino acids, fatty acids, rich trace elements and carbohydrates. With the increase of the per capita pork consumption of Chinese residents, people's pursuit of pork has gradually shifted from "quantity" to "quality". The meat quality and taste are generally poor, far inferior to that of local pigs in China. Therefore, how to ensure the "quantity" of pork while taking into account the "quality" of pork has become a common concern of consumers and has become an important research component. Research hotspot of genetic improvement of breeding pigs to improve meat quality. [0003] As one of the five basic tastes for evaluating foo...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11C12N15/90A01K67/027
CPCA01K67/0276A01K2217/07A01K2227/108A01K2267/02C12N15/907C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 黄路生麻骏武黄聪郑敏肖石军杨斌
Owner JIANGXI AGRICULTURAL UNIVERSITY
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